LEPRE1 (P3H1) (NM_001243246) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GROS1; LEPRE1; OI8
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331978 representing NM_001243246. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGTACGCGCGTTGAAGCTGCTGACCACACTGCTGGCTGTCGTGGCCGCTGCCTCCCAAGCCGAG GTCGAGTCCGAGGCAGGATGGGGCATGGTGACGCCTGATCTGCTCTTCGCCGAGGGGACCGCAGCCTAC GCGCGCGGGGACTGGCCCGGGGTGGTCCTGAGCATGGAACGGGCGCTGCGCTCCCGGGCAGCCCTCCGC GCCCTTCGCCTGCGCTGCCGCACCCAGTGTGCCGCCGACTTCCCGTGGGAGCTGGACCCCGACTGGTCC CCCAGCCCGGCCCAGGCCTCGGGCGCCGCCGCCCTGCGCGACCTGAGCTTCTTCGGGGGCCTTCTGCGT CGCGCTGCCTGCCTGCGCCGCTGCCTCGGGCCGCCGGCCGCCCACTCGCTCAGCGAAGAGATGGAGCTG GAGTTCCGCAAGCGGAGCCCCTACAACTACCTGCAGGTCGCCTACTTCAAGATCAACAAGTTGGAGAAA GCTGTTGCTGCAGCACACACCTTCTTCGTGGGCAATCCTGAGCACATGGAAATGCAGCAGAACCTAGAC TATTACCAAACCATGTCTGGAGTGAAGGAGGCCGACTTCAAGGATCTTGAGACTCAACCCCATATGCAA GAATTTCGACTGGGAGTGCGACTCTACTCAGAGGAACAGCCACAGGAAGCTGTGCCCCACCTAGAGGCG GCGCTGCAAGAATACTTTGTGGCCTATGAGGAGTGCCGTGCCCTCTGCGAAGGGCCCTATGACTACGAT GGCTACAACTACCTTGAGTACAACGCTGACCTCTTCCAGGCCATCACAGATCATTACATCCAGGTCCTC AACTGTAAGCAGAACTGTGTCACGGAGCTTGCTTCCCACCCAAGTCGAGAGAAGCCCTTTGAAGACTTC CTCCCATCGCATTATAATTATCTGCAGTTTGCCTACTATAACATTGGGAATTATACACAGGCTGTTGAA TGTGCCAAGACCTATCTTCTCTTCTTCCCCAATGACGAGGTGATGAACCAAAATTTGGCCTATTATGCA GCTATGCTTGGAGAAGAACACACCAGATCCATCGGCCCCCGTGAGAGTGCCAAGGAGTACCGACAGCGA AGCCTACTGGAAAAAGAACTGCTTTTCTTCGCTTATGATGTTTTTGGAATTCCCTTTGTGGATCCGGAT TCATGGACTCCAGAAGAAGTGATTCCCAAGAGATTGCAAGAGAAACAGAAGTCAGAACGGGAAACAGCC GTACGCATCTCCCAGGAGATTGGGAACCTTATGAAGGAAATCGAGACCCTTGTGGAAGAGAAGACCAAG GAGTCACTGGATGTGAGCAGACTGACCCGGGAAGGTGGCCCCCTGCTGTATGAAGGCATCAGTCTCACC ATGAACTCCAAACTCCTGAATGGTTCCCAGCGGGTGGTGATGGACGGCGTAATCTCTGACCACGAGTGT CAGGAGCTGCAGAGACTGACCAATGTGGCAGCAACCTCAGGAGATGGCTACCGGGGTCAGACCTCCCCA CATACTCCCAATGAAAAGTTCTATGGTGTCACTGTCTTCAAAGCCCTCAAGCTGGGGCAAGAAGGCAAA GTTCCTCTGCAGAGTGCCCACCTGTACTACAACGTGACGGAGAAGGTGCGGCGCATCATGGAGTCCTAC TTCCGCCTGGATACGCCCCTCTACTTTTCCTACTCTCATCTGGTGTGCCGCACTGCCATCGAAGAGGTC CAGGCAGAGAGGAAGGATGATAGTCATCCAGTCCACGTGGACAACTGCATCCTGAATGCCGAGACCCTC GTGTGTGTCAAAGAGCCCCCAGCCTACACCTTCCGCGACTACAGCGCCATCCTTTACCTAAATGGGGAC TTCGATGGCGGAAACTTTTATTTCACTGAACTGGATGCCAAGACCGTGACGGCAGAGGTGCAGCCTCAG TGTGGAAGAGCCGTGGGATTCTCTTCAGGCACTGAAAACCCACATGGAGTGAAGGCTGTCACCAGGGGG CAGCGCTGTGCCATCGCCCTGTGGTTCACCCTGGACCCTCGACACAGCGAGCGGGTGAGAGCAGCTCGA GCGGGTGAGAGCAGCTGGTGCTGTGGTGACCCGTTCCCAGAGCGCCCTTGGTTTGCCTTTCTCTTCCCC AAATCCCATTGCCAGTGGCTGAGACACGAAAGGAGCACTTGGGACACCAGCTCCAACGCCCTGTCATTA TGGTCACATTGCCTTGTCCTCCCTGGGCCTGCTGTGAACGGGATCCAGGTGGGGAAAGAGGTCAAGACA GGGAGCGATGCTGAGTTCTTGGTTCCCTCCTTGGGCCCCACTTCAGCTGTCCTTTTCCAGAGAGTAGGA CCTGCTGGGAAGGAGATGAGCCTGGGGCCATTAAGGAACCTTCCTTGTCCCCTGGGAAGTAGCAGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243246
Insert Size	2415 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243246.1
RefSeq Size	3115 bp
RefSeq ORF	2415 bp
Locus ID	64175
UniProt ID	Q32P28
Protein Families	Secreted Protein
MW	90.6 kDa
Gene Summary	This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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