NFIC (NM_001245005) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CTF; CTF5; NF-I; NFI
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332116 representing NM_001245005. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGATGAGTTCCACCCGTTCATCGAGGCCCTGCTGCCTCACGTCCGCGCCTTCGCCTACACCTGGTTC AACCTGCAGGCGCGGAAGCGCAAGTACTTCAAGAAGCACGAGAAGCGGATGTCGAAGGACGAGGAGCGT GCGGTCAAGGACGAGCTGCTGGGCGAGAAGCCCGAGGTCAAGCAGAAGTGGGCGTCGCGGCTGCTGGCC AAGCTGCGCAAGGACATCCGGCCCGAGTGCCGCGAGGACTTCGTGCTGAGCATCACCGGCAAGAAGGCG CCGGGCTGCGTGCTCTCCAACCCCGACCAGAAGGGCAAGATGCGGCGCATCGACTGTCTCCGGCAGGCG GACAAGGTGTGGCGGCTGGACCTGGTCATGGTCATCCTGTTCAAGGGCATCCCGCTGGAGAGCACCGAC GGCGAGCGCCTGGTCAAGGCTGCGCAGTGCGGTCACCCGGTCCTGTGCGTGCAGCCGCACCACATTGGC GTGGCCGTCAAGGAGCTGGACCTCTACCTGGCCTACTTCGTGCGTGAGCGAGATGCAGAGCAAAGCGGC AGTCCCCGGACAGGGATGGGCTCTGACCAGGAGGACAGCAAGCCCATCACGCTGGACACGACCGACTTC CAGGAGAGCTTTGTCACCTCCGGCGTGTTCAGCGTCACTGAGCTCATCCAAGTGTCCCGGACACCCGTG GTGACTGGAACAGGACCCAACTTCTCCCTGGGGGAGCTGCAGGGGCACCTGGCATACGACCTGAACCCA GCCAGCACTGGCCTCAGAAGAACGCTGCCCAGCACCTCCTCCAGTGGGAGCAAGCGGCACAAATCGGGC TCGATGGAGGAAGACGTGGACACGAGCCCTGGCGGCGATTACTACACTTCGCCCAGCTCGCCCACGAGT AGCAGCCGCAACTGGACGGAGGACATGGAAGGAGGCATCTCGTCCCCGGTGAAGAAGACAGAGATGGAC AAGTCACCATTCAACAGCCCGTCCCCCCAGGACTCTCCCCGCCTCTCCAGCTTCACCCAGCACCACCGG CCCGTCATCGCCGTGCACAGCGGGATCGCCCGGAGCCCACACCCGTCCTCCGCTCTGCATTTCCCTACG ACGTCCATCCTACCCCAGACGGCCTCCACCTACTTCCCCCACACGGCCATCCGCTACCCACCTCATCTC AACCCCCAGGACCCGCTCAAAGATCTTGTCTCGCTGGCCTGCGACCCAGCCAGCCAGCAACCTGGACCG CCTACTCTCCGCCCGACACGTCCCCTGCAAACCGTTCCTTTGTGGGATTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001245005
Insert Size	1293 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001245005.1
RefSeq Size	7932 bp
RefSeq ORF	1293 bp
Locus ID	4782
UniProt ID	P08651
Protein Families	Transcription Factors
MW	47.9 kDa
Gene Summary	The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (4) contains an alternate 5' terminal exon, and lacks an exon in the 3' coding region (causing a frame-shift) compared to variant 1. This results in translation initiation from an alternate start codon, and a shorter isoform (4) with distinct N- and C- termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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