TUG (ASPSCR1) (NM_001251888) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASPCR1; ASPL; ASPS; RCC17; TUG; UBXD9; UBXN9
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332145 representing NM_001251888. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGCCCCGGCAGGCGGCGGAGGCTCCGCGGTGTCGGTGCTGGCCCCGAACGGCCGGCGCCACACG GTGAAGGTGACGCCGAGCACCGTGCTGCTTCAGGTTCTGGAGGACACGTGCCGGCGGCAGGACTTCAAC CCCTGTGAATATGATCTGAAGTTTCAGAGGAGCGTGCTCGACCTTTCTCTCCAGTGGAGATTTGCCAAC CTGCCCAACAATGCCAAGCTGGAGATGGTGCCCGCTTCCCGGAGCCGTGAGGGGCCTGAGAACATGGTT CGCATCGCTTTGCAGCTGGACGATGGCTCGAGGTTGCAGGACTCTTTCTGTTCAGGCCAGACCCTCTGG GAGCTTCTCAGCCATTTTCCACAGATCAGGGAGTGCCTGCAGCACCCCGGCGGGGCCACCCCAGTCTGC GTGTACACGAGGGATGAGGTGACGGGTGAAGCTGCCCTGCGGGGCACGACGCTGCAGTCGCTGGGCCTG ACCGGGGGCAGCGCCACCATCAGGTTTGTCATGAAGTGCTACGACCCCGTGGGCAAGACCCCAGGAAGC CTGGGCTCGTCAGCGTCGGCTGGCCAGGCAGCCGCCAGCGCTCCACTTCCCTTGGAATCTGGGGAGCTC AGCCGCGGCGACTTGAGCCGTCCGGAGGACGCGGACACCTCAGGGCCCTGCTGCGAGCACACTCAGGAG AAGCAGAGCACAAGGGCACCCGCAGCTGCCCCCTTTGTTCCTTTCTCGGGTGGGGGACAGAGACTGGGG GGCCCTCCTGGGCCCACGAGGCCTCTGACATCATCTTCAGCTAAGTTGCCGAAGTCCCTCTCCAGCCCT GGAGGCCCCTCCAAGCCAAAGAAGTCCAAGTCGGGCCAGGATCCCCAGCAGGAGCAGGAGCAGGAGCGG GAGCGGGATCCCCAGCAGGAGCAGGAGCGGGAGCGGCCCGTGGACCGGGAGCCCGTGGACCGGGAGCCG GTGGTGTGCCACCCCGACCTGGAGGAGCGGCTGCAGGCCTGGCCAGCGGAGCTGCCTGATGAGTTCTTT GAGCTGACGGTGGACGACGTGAGAAGACGCTTGGCCCAGCTCAAGAGTGAGCGGAAGCGCCTGGAAGAA GCCCCCTTGGTGACCAAGGCCTTCAGGGAGGCGCAGATAAAGGAGAAGCTGGAGCGCTACCCAAAGGTG GCTCTGAGGGTCCTGTTCCCCGACCGCTACGTCCTACAGGGCTTCTTCCGCCCCAGCGAGACAGTGGGG GACTTGCGAGACTTCGTGAGGAGCCACCTGGGGAACCCCGAGCTGTCATTTTACCTGTTCATCACCCCT CCAAAAACAGTCCTGGACGACCACACGCAGACCCTCTTTCAGCCCCAGCTTGGTGACCGGGTGGCTCCA TTCACCCTGGGTCCCTCGCTGAAACGGTGCCTGGGACCAGAGCAGAGAACACGCTTGCCAGTGGTAGGA GATGGAGGCGACGTGGACTCTGGGAGGCTTCTTTTTTGGGGTCCATCCAGAGGCCGAGCCTCTCCAAGC ACTGGTCAGCCTCCATGCCACCCAGTCTGCCGACCCTCCTCTCCACCATCCCCTCGTCCGAGCAGTGGC GATCCCTCCCGAGTCAAGGCTGGGCACAAGCACGTGGGGACAGGCCGGGCGAACCTCTTCCCGGCCGCT CTGGTGCACTTGGGAGCCGAGGAGCCGGCAGGTGTCTACCTGGAGCCTGGCCTGCTGGAGCATGCCATC TCCCCATCTGCGGCCGATGTGCTGGTGGCCAGGTACATGTCCAGGGCCGCCGGGTCCCCTTCCCCATTG CCAGCCCCTGACCCTGCACCTAAGTCTGAGCCAGCTGCTGAGGAGGGGGCGCTGGTCCCCCCTGAGCCC ATCCCAGGGACGGCCCAGCCCGTGAAGAGGAGCCTGGGCAAGGTGCCCAAGTGGCTGAAGCTGCCGGCC AGCAAGAGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001251888
Insert Size	1944 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001251888.1
RefSeq Size	2140 bp
RefSeq ORF	1944 bp
Locus ID	79058
UniProt ID	Q9BZE9
MW	70 kDa
Gene Summary	The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (2) has an additional in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) is longer than isoform 1.

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