ARHGAP22 (NM_001256025) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RhoGAP2; RhoGap22
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332288 representing NM_001256025. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTGCCGACAGCTTCCAGCAAAAGGAGGACGTTTGCAGCCAGATATTTCACCCGCTCCAAAAGCCTA GTGATGGGGGAGCAGAGCCGGAGCCCTGGGCGGATGCCGTGCCCTCACAGGCTGGGCCCCGTGCTGAAG GCGGGCTGGCTGAAGAAGCAGAGGAGCATCATGAAGAACTGGCAGCAGCGCTGGTTTGTGCTGCGTGGG GATCAGCTTTTCTACTACAAGGACAAAGATGAGATCAAGCCCCAGGGATTTATTTCTCTACAAGGGACA CAGGTGACTGAACTTCCTCCTGGCCCCGAGGACCCAGGGAAGCACCTCTTTGAGATCAGCCCAGGTGGT GCCGGGGAGCGGGAGAAGGTGCCGGCCAACCCCGAGGCGCTCCTGCTCATGGCCAGCTCCCAGCGTGAC ATGGAGGACTGGGTGCAGGCCATCCGCCGAGTCATCTGGGCCCCGCTGGGCGGAGGGATCTTTGGGCAG CGCCTAGAGGAAACAGTCCACCACGAGCGGAAGTATGGCCCCCGCCTGGCGCCCCTGCTGGTGGAGCAG TGTGTGGACTTCATCCGGGAGCGCGGGCTCACTGAGGAGGGGCTGTTCCGCATGCCAGGCCAGGCCAAC CTGGTGAGGGACCTGCAGGATTCCTTCGACTGTGGGGAGAAGCCACTGTTTGACAGCACAACAGACGTG CACACGGTGGCCTCCCTGCTGAAGCTGTACCTGCGGGAGCTCCCCGAGCCCGTGGTCCCCTTCGCCAGG TACGAGGACTTCCTCAGCTGCGCCCAGCTGCTCACCAAGGACGAGGGGGAGGGCACTCTGGAGTTGGCT AAACAAGTGAGCAACCTTCCTCAGGCAAATTACAACCTGCTCAGATACATCTGCAAGTTTCTGGATGAA GTTCAGGCATACTCAAATGTCAACAAGATGAGTGTCCAGAATCTGGCAACCGTTTTTGGACCTAACATT CTGCGGCCACAGGTAGAGGACCCAGTAACCATCATGGAAGGCACTTCCCTCGTCCAGCACCTGATGACC GTCCTCATCCGCAAACACAGCCAGCTCTTCACGGCACCGGTCCCGGAAGGGCCCACCTCCCCGCGCGGG GGCCTGCAATGCGCAGTGGGGTGGGGCTCCGAGGAGGTCACCAGGGACAGCCAAGGAGAGCCCGGCGGC CCCGGCCTGCCCGCGCACAGGACCTCTTCCCTGGACGGGGCGGCCGTGGCGGTGCTCTCCAGAACAGCC CCCACGGGGCCGGGGAGCCGGTGCAGCCCTGGGAAGAAGGTGCAGACCCTGCCCAGTTGGAAGTCCTCC TTCCGGCAGCCGAGGTCCCTATCGGGAAGCCCGAAGGGGGGCGGCTCATCCCTGGAGGTGCCCATCATC TCCTCCGGCGGGAACTGGCTTATGAACGGGCTGTCCTCCCTGCGCGGACACCGCCGGGCCTCGTCGGGA GACCGGCTCAAGGACTCGGGCTCCGTGCAGAGACTCTCCACCTACGACAATGTGCCCGCGCCGGGCCTG GTCCCCGGCATACCCAGCGTGGCCAGTATGGCGTGGTCCGGGGCCTCGTCCAGCGAGTCGTCGGTGGGG GGCTCACTCAGCAGCTGCACGGCCTGCCGCGCCAGCGACTCGTCTGCCCGCAGTTCCCTGCACACCGAC TGGGCCCTGGAGCCCTCCCCGCTCCCCAGCAGCAGCGAGGACCCCAAGTCCCTGGACCTGGACCACAGC ATGGACGAGGCGGGCGCGGGTGCCAGCAACAGCGAGCCCAGCGAGCCGGACAGCCCCACCCGGGAACAC GCGCGCCGCTCCGAGGCCTTACAGGGGCTGGTCACTGAGCTCAGGGCCGAGCTGTGCCGCCAGCGGACT GAGTACGAGAGGAGTGTGAAAAGAATCGAAGAAGGGAGTGCTGACCTGAGAAAACGAATGTCCCGGTTA GAAGAAGAACTGGACCAGGAAAAGAAAAAATACATCATGCTGGAAATAAAGCTGCGGAACTCTGAACGG GCGCGGGAGGATGCGGAGAGGAGGAACCAGCTGTTGCAGAGGGAAATGGAGGAGTTTTTTTCGACCCTA GGAAGCTTGACTGTTGGGGCAAAAGGTGCCAGGGCCCCAAAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256025
Insert Size	2115 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256025.2
RefSeq Size	2712 bp
RefSeq ORF	2115 bp
Locus ID	58504
UniProt ID	Q7Z5H3
MW	77.5 kDa
Gene Summary	This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (2) uses an alternate 5' exon and thus differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Variants 2 and 10 both encode the same isoform (2).

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