NKCC1 (SLC12A2) (NM_001256461) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BSC; BSC2; KILQS; NKCC1; PPP1R141
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332389 representing NM_001256461. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCCGCGGCCCACGGCGCCCTCCTCCGGCGCCCCGGGACTGGCCGGGGTCGGGGAGACGCCGTCA GCCGCTGCGCTGGCCGCAGCCAGGGTGGAACTGCCCGGCACGGCTGTGCCCTCGGTGCCGGAGGATGCT GCGCCCGCGAGCCGGGACGGCGGCGGGGTCCGCGATGAGGGCCCCGCGGCGGCCGGGGACGGGCTGGGC AGACCCTTGGGGCCCACCCCGAGCCAGAGCCGTTTCCAGGTGGACCTGGTTTCCGAGAACGCCGGGCGG GCCGCTGCTGCGGCGGCGGCGGCGGCGGCGGCAGCGGCGGCGGCTGGTGCTGGGGCGGGGGCCAAGCAG ACCCCCGCGGACGGGGAAGCCAGCGGCGAGAGCGAGCCGGCTAAAGGCAGCGAGGAAGCCAAGGGCCGC TTCCGCGTGAACTTCGTGGACCCAGCTGCCTCCTCGTCGGCTGAAGACAGCCTGTCAGATGCTGCCGGG GTCGGAGTCGACGGGCCCAACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGC CTGCACTCCGGCGGCGGCGGCGGCAGTGGGCACCACCAGCACTACTATTATGATACCCACACCAACACC TACTACCTGCGCACCTTCGGCCACAACACCATGGACGCTGTGCCCAGGATCGATCACTACCGGCACACA GCCGCGCAGCTGGGCGAGAAGCTGCTCCGGCCTAGCCTGGCGGAGCTCCACGACGAGCTGGAAAAGGAA CCTTTTGAGGATGGCTTTGCAAATGGGGAAGAAAGTACTCCAACCAGAGATGCTGTGGTCACGTATACT GCAGAAAGTAAAGGAGTCGTGAAGTTTGGCTGGATCAAGGGTGTATTAGTACGTTGTATGTTAAACATT TGGGGTGTGATGCTTTTCATTAGATTGTCATGGATTGTGGGTCAAGCTGGAATAGGTCTATCAGTCCTT GTAATAATGATGGCCACTGTTGTGACAACTATCACAGGATTGTCTACTTCAGCAATAGCAACTAATGGA TTTGTAAGAGGAGGAGGAGCATATTATTTAATATCTAGAAGTCTAGGGCCAGAATTTGGTGGTGCAATT GGTCTAATCTTCGCCTTTGCCAACGCTGTTGCAGTTGCTATGTATGTGGTTGGATTTGCAGAAACCGTG GTGGAGTTGCTTAAGGAACATTCCATACTTATGATAGATGAAATCAATGATATCCGAATTATTGGAGCC ATTACAGTCGTGATTCTTTTAGGTATCTCAGTAGCTGGAATGGAGTGGGAAGCAAAAGCTCAGATTGTT CTTTTGGTGATCCTACTTCTTGCTATTGGTGATTTCGTCATAGGAACATTTATCCCACTGGAGAGCAAG AAGCCAAAAGGGTTTTTTGGTTATAAATCTGAAATATTTAATGAGAACTTTGGGCCCGATTTTCGAGAG GAAGAGACTTTCTTTTCTGTATTTGCCATCTTTTTTCCTGCTGCAACTGGTATTCTGGCTGGAGCAAAT ATCTCAGGTGATCTTGCAGATCCTCAGTCAGCCATACCCAAAGGAACACTCCTAGCCATTTTAATTACT ACATTGGTTTACGTAGGAATTGCAGTATCTGTAGGTTCTTGTGTTGTTCGAGATGCCACTGGAAACGTT AATGACACTATCGTAACAGAGCTAACAAACTGTACTTCTGCAGCCTGCAAATTAAACTTTGATTTTTCA TCTTGTGAAAGCAGTCCTTGTTCCTATGGCCTAATGAACAACTTCCAGGTAATGAGTATGGTGTCAGGA TTTACACCACTAATTTCTGCAGGTATATTTTCAGCCACTCTTTCTTCAGCATTAGCATCCCTAGTGAGT GCTCCCAAAATATTTCAGGCTCTATGTAAGGACAACATCTACCCAGCTTTCCAGATGTTTGCTAAAGGT TATGGGAAAAATAATGAACCTCTTCGTGGCTACATCTTAACATTCTTAATTGCACTTGGATTCATCTTA ATTGCTGAACTGAATGTTATTGCACCAATTATCTCAAACTTCTTCCTTGCATCATATGCATTGATCAAT TTTTCAGTATTCCATGCATCACTTGCAAAATCTCCAGGATGGCGTCCTGCATTCAAATACTACAACATG TGGATATCACTTCTTGGAGCAATTCTTTGTTGCATAGTAATGTTCGTCATTAACTGGTGGGCTGCATTG CTAACATATGTGATAGTCCTTGGGCTGTATATTTATGTTACCTACAAAAAACCAGATGTGAATTGGGGA TCCTCTACACAAGCCCTGACTTACCTGAATGCACTGCAGCATTCAATTCGTCTTTCTGGAGTGGAAGAC CACGTGAAAAACTTTAGGCCACAGTGTCTTGTTATGACAGGTGCTCCAAACTCACGTCCAGCTTTACTT CATCTTGTTCATGATTTCACAAAAAATGTTGGTTTGATGATCTGTGGCCATGTACATATGGGTCCTCGA AGACAAGCCATGAAAGAGATGTCCATCGATCAAGCCAAATATCAGCGATGGCTTATTAAGAACAAAATG AAGGCATTTTATGCTCCAGTACATGCAGATGACTTGAGAGAAGGTGCACAGTATTTGATGCAGGCTGCT GGTCTTGGTCGTATGAAGCCAAACACACTTGTCCTTGGATTTAAGAAAGATTGGTTGCAAGCAGATATG AGGGATGTGGATATGTATATAAACTTATTTCATGATGCTTTTGACATACAATATGGAGTAGTGGTTATT CGCCTAAAAGAAGGTCTGGATATATCTCATCTTCAAGGACAAGAAGAATTATTGTCATCACAAGAGAAA TCTCCTGGCACCAAGGATGTGGTAGTAAGTGTGGAATATAGTAAAAAGTCCGATTTAGATACTTCCAAA CCACTCAGTGAAAAACCAATTACACACAAAGAATCCAAAGGCCCTATTGTGCCTTTAAATGTAGCTGAC CAAAAGCTTCTTGAAGCTAGTACACAGTTTCAGAAAAAACAAGGAAAGAATACTATTGATGTCTGGTGG CTTTTTGATGATGGAGGTTTGACCTTATTGATACCTTACCTTCTGACGACCAAGAAAAAATGGAAAGAC TGTAAGATCAGAGTATTCATTGGTGGAAAGATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACT TTGCTTAGCAAGTTCCGGATAGACTTTTCTGATATCATGGTTCTAGGAGATATCAATACCAAACCAAAG AAAGAAAATATTATAGCTTTTGAGGAAATCATTGAGCCATACAGACTTCATGAAGATGATAAAGAGCAA GATATTGCAGATAAAATGAAAGAAGATGAACCATGGCGAATAACAGATAATGAGCTTGAACTTTATAAG ACCAAGACATACCGGCAGATCAGGTTAAATGAGTTATTAAAGGAACATTCAAGCACAGCTAATATTATT GTCATGAGTCTCCCAGTTGCACGAAAAGGTGCTGTGTCTAGTGCTCTCTACATGGCATGGTTAGAAGCT CTATCTAAGGACCTACCACCAATCCTCCTAGTTCGTGGGAATCATCAGAGTGTCCTTACCTTCTATTCA TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256461
Insert Size	3591 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256461.1
RefSeq Size	6843 bp
RefSeq ORF	3591 bp
Locus ID	6558
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Vibrio cholerae infection
MW	129.7 kDa
Gene Summary	The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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