Cullin 3 (CUL3) (NM_001257198) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CUL-3; NEDAUS; PHA2E
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332520 representing NM_001257198. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTAAGAATTCACTTCTTTTCATTTAGTTTTAATTTGTCTCATGATGTAGTTCTCTCAGTAATCACA GAACCTTCAAGATGTATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCA ATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACA ATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATA AATAAGGTGCGAGAAGATGTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGG AATGATCATCAAACAGCTATGGTGATGATTAGAGACATACTAATGTACATGGACCGTGTGTATGTACAA CAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGG TGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTA GACAGAGGCGCAATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTAT GAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTT TTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAATGAAGAAATAGAACGA GTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCC AAGCACATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACA GAAGACCTTGGTTGCATGTACAAGTTATTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGT ATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAAGGAGAAGGAAAGAATCCTGTT GACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAAT GACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCT GAATACCTCTCATTATTTATTGATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTA GAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATGCAAGAAAAAGATGTATTTGAACGTTAT TATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAAAACATG ATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATG AGCATCTCAAACACAACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGT GTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGCCCACTCAGTCAGCCACACCAAAGTGCAAC ATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAACACAGTGGTCGA CAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAG GAAGATGGATCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTG CAAGTTTCCACTTTCCAGATGACCATATTAATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGAA ATTCAGCAAGAGACAGATATCCCTGAAAGAGAGCTTGTTAGAGCCCTACAGTCCCTCGCCTGTGGTAAA CCAACACAGCGGGTTCTTACAAAAGAACCCAAATCAAAGGAAATAGAAAATGGTCATATATTTACAGTT AATGATCAATTCACATCCAAACTACACAGAGTCAAGATTCAAACAGTTGCTGCCAAACAAGGTGAATCC GACCCAGAGAGGAAAGAAACAAGGCAGAAAGTAGACGACGACAGAAAACATGAGATAGAAGCTGCTATA GTGCGGATAATGAAATCTAGAAAGAAGATGCAGCACAATGTTCTAGTAGCGGAGGTAACTCAGCAGTTG AAGGCGCGATTCTTACCAAGTCCAGTTGTTATTAAGAAACGTATTGAAGGACTTATTGAGAGAGAATAT TTGGCACGAACACCTGAGGATCGCAAAGTATACACATATGTAGCATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001257198
Insert Size	2325 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257198.1
RefSeq Size	6473 bp
RefSeq ORF	2325 bp
Locus ID	8452
UniProt ID	Q13618
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	89.7 kDa
Gene Summary	This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is longer and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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