SLC4A7 (NM_001258379) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NBC2; NBC3; NBCN1; SBC2; SLC4A6
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332682 representing NM_001258379. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGGCTGATGGGGCCGGCGAGCAGATGAGACCGCTACTCACCCGGGGTCCTGATGAAGAAGCTGTT GTGGATCTTGGCAAAACTAGCTCAACTGTGAACACCAAGTTTGAAAAAGAAGAACTAGAAAGTCATAGA GCTGTATATATTGGTGTTCACGTCCCGTTTAGTAAAGAGAGTCGTCGGCGTCATAGGCATCGCGGACAC AAACATCACCACCGGAGAAGAAAAGATAAAGAATCAGATAAAGAAGATGGACGGGAATCTCCTTCTTAT GATACACCATCCCAGAGAGTTCAGTTTATCCTTGGTACTGAAGATGATGATGAAGAACATATTCCCCAT GATCTCTTCACGGAAATGGATGAACTGTGTTACAGAGATGGAGAAGAATATGAATGGAAAGAAACTGCT AGATGGCTGAAATTTGAAGAGGATGTTGAAGATGGCGGTGACCGATGGAGTAAACCTTATGTGGCAACT CTCTCTTTGCACAGTCTTTTTGAACTAAGGAGTTGCATCCTCAATGGAACAGTCATGCTGGATATGAGA GCAAGCACTCTAGATGAAATAGCAGATATGGTATTAGACAACATGATAGCTTCTGGCCAATTAGACGAG TCCATACGAGAGAATGTCAGAGAAGCTCTTCTGAAGAGACATCATCATCAGAATGAGAAAAGATTCACC AGTCGGATTCCTCTTGTTCGATCTTTTGCAGATATAGGCAAGAAACATTCTGACCCTCACTTGCTTGAA AGGAATGGTATTTTGGCCTCTCCCCAGTCTGCTCCTGGAAACTTGGACAATAGTAAAAGTGGAGAAATT AAAGGTAATGGAAGTGGTGGAAGCAGAGAAAATAGTACTGTTGACTTCAGCAAGGTTGATATGAATTTC ATGAGAAAAATTCCTACGGGTGCTGAGGCATCCAACGTCCTGGTGGGCGAAGTAGACTTTTTGGAAAGG CCAATAATTGCATTTGTGAGACTGGCTCCTGCTGTCCTCCTTACAGGGTTGACTGAGGTCCCTGTTCCA ACCAGGTTTTTGTTTTTGTTATTGGGTCCAGCGGGCAAGGCACCACAGTACCATGAAATTGGACGATCA ATAGCCACTCTCATGACAGATGAGATTTTCCATGATGTAGCTTATAAAGCAAAAGACAGAAATGACCTC TTATCTGGAATTGATGAATTTTTAGATCAAGTAACTGTCCTACCTCCAGGAGAGTGGGATCCTTCTATA CGCATAGAACCACCAAAAAGTGTCCCTTCTCAGGAAAAGAGAAAGATTCCTGTGTTTCACAATGGATCT ACCCCCACACTGGGTGAGACTCCTAAAGAGGCCGCTCATCATGCTGGGCCTGAGCTACAGAGGACTGGA CGGCTTTTTGGTGGTTTGATACTTGACATCAAAAGGAAAGCACCTTTTTTCTTGAGTGACTTCAAGGAT GCATTAAGCCTGCAGTGCCTGGCCTCGATTCTTTTCCTATACTGTGCCTGTATGTCTCCTGTAATCACT TTTGGAGGGCTGCTTGGAGAAGCTACAGAAGGCAGAATAAGTGCAATAGAGTCTCTTTTTGGAGCATCA TTAACTGGGATTGCCTATTCATTGTTTGCTGGGCAACCTCTAACAATATTGGGGAGCACAGGTCCAGTT CTAGTGTTTGAAAAAATTTTATATAAATTCTGCAGAGATTATCAACTTTCTTATCTGTCTTTAAGAACC AGTATTGGTCTGTGGACTTCTTTTTTGTGCATTGTTTTGGTTGCAACAGATGCAAGCAGCCTTGTGTGT TATATTACTCGATTTACAGAAGAGGCTTTTGCAGCCCTTATTTGCATCATATTCATCTACGAGGCTTTG GAGAAGCTCTTTGATTTAGGAGAAACATATGCATTTAATATGCACAACAACTTAGATAAACTGACCAGC TACTCATGTGTATGTACTGAACCTCCAAACCCCAGCAATGAAACTCTAGCACAATGGAAGAAAGATAAT ATAACAGCACACAATATTTCCTGGAGAAATCTTACTGTTTCTGAATGTAAAAAACTTCGTGGTGTATTC TTGGGGTCAGCTTGTGGTCATCATGGACCTTATATTCCAGATGTGCTCTTTTGGTGTGTCATCTTGTTT TTCACAACATTTTTTCTGTCTTCATTCCTCAAGCAATTTAAGACCAAGCGTTACTTTCCTACCAAGGTG CGATCGACAATCAGTGATTTTGCTGTATTTCTCACAATAGTAATAATGGTTACAATTGACTACCTTGTA GGAGTTCCATCTCCTAAACTTCATGTTCCTGAAAAATTTGAGCCTACTCATCCAGAGAGAGGGTGGATC ATAAGCCCACTGGGAGATAATCCTTGGTGGACCTTATTAATAGCTGCTATTCCTGCTTTGCTTTGTACC ATTCTCATCTTTATGGATCAACAAATCACAGCTGTAATTATAAACAGAAAGGAACACAAATTGAAGAAA GGAGCTGGCTATCACCTTGATTTGCTCATGGTTGGCGTTATGTTGGGAGTTTGCTCTGTCATGGGACTT CCATGGTTTGTGGCTGCAACAGTGTTGTCAATAAGTCATGTCAACAGCTTAAAAGTTGAATCTGAATGT TCTGCTCCAGGGGAACAACCCAAGTTTTTGGGAATTCGTGAACAGCGGGTTACAGGGCTAATGATTTTT ATTCTAATGGGCCTCTCTGTGTTCATGACTTCAGTCCTAAAGTTTATTCCAATGCCTGTTCTGTATGGT GTTTTCCTTTATATGGGAGTTTCCTCATTAAAAGGAATCCAGTTATTTGACCGTATAAAATTATTTGGA ATGCCTGCTAAGCATCAGCCTGATTTGATATACCTCCGTTATGTGCCGCTCTGGAAGGTCCATATTTTC ACAGTCATTCAGCTTACTTGTTTGGTCCTTTTATGGGTGATAAAAGTTTCAGCTGCTGCAGTGGTTTTT CCCATGATGGTTCTTGCATTAGTGTTTGTGCGCAAACTCATGGACCTGTGTTTCACGAAGAGAGAACTT AGTTGGCTTGATGATCTTATGCCAGAAAGTAAGAAAAAGAAAGAAGATGACAAAAAGAAAAAAGAGAAA GAGGAAGCTGAACGGATGCTTCAAGATGATGATGATACTGTGCACCTTCCATTTGAAGGGGGAAGTCTC TTGCAAATTCCAGTCAAGGCCCTAAAATATAGTGTTGATCCCTCAATTGTTAACATATCAGATGAAATG GCCAAAACTGCACAGTGGAAGGCACTTTCCATGAATACTGAGAATGCCAAAGTAACCAGATCTAACATG AGTCCTGATAAACCTGTGAGTGTGAAAATAAGTTTTGAAGATGAACCAAGAAAGAAATACGTGGATGCT GAAACTTCATTATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001258379
Insert Size	3396 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258379.1
RefSeq Size	7741 bp
RefSeq ORF	3396 bp
Locus ID	9497
UniProt ID	Q9Y6M7
Protein Families	Druggable Genome, Transmembrane
MW	127.4 kDa
Gene Summary	This locus encodes a sodium bicarbonate cotransporter. The encoded transmembrane protein appears to transport sodium and bicarbonate ions in a 1:1 ratio, and is thus considered an electroneutral cotransporter. The encoded protein likely plays a critical role in regulation of intracellular pH involved in visual and auditory sensory transmission. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. This variant has additional differences in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a.

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