RTN3 (NM_001265589) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASYIP; HAP; NSPL2; NSPLII; RTN3-A1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332810 representing NM_001265589. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGAGCCGTCGGCGGCCACTCAGTCCCATTCCATCTCCTCGTCGTCCTTCGGAGCCGAGCCGTCC GCGCCCGGCGGCGGCGGGAGCCCAGGAGCCTGCCCCGCCCTGGGGACGAAGAGCTGCAGCTCCTCCTGT GCGGATTCCTTTGTTTCTTCCTCTTCCTCTCAGCCTGTATCTCTATTTTCGACCTCACAAGAGGGATTG AGCTCTCTTTGCTCTGATGAGCCATCTTCAGAAATTATGACTTCTTCCTTTCTTTCATCTTCTGAAATA CATAACACTGGCCTTACAATACTACATGGAGAAAAAAGCCATGTGTTAGGGAGCCAGCCTATTTTAGCC AAAGAAGGAAAAGACCACTTGGATCTTCTAGATATGAAAAAGATGGAAAAGCCTCAGGGGACCAGCAAC AACGTATCAGACTCTTCAGTTTCTCTTGCAGCAGGAGTTCATTGTGACCGTCCTTCTATTCCAGCCAGT TTCCCAGAGCATCCTGCTTTTCTCTCAAAGAAAATTGGTCAAGTGGAAGAGCAAATAGATAAAGAGACC AAGAACCCAAATGGGGTATCAAGTAGGGAGGCTAAAACTGCATTGGATGCTGATGACAGATTCACTTTG CTGACAGCCCAGAAACCACCTACTGAGTACTCTAAGGTAGAAGGCATTTATACATATTCTTTGTCTCCA TCCAAAGTTTCAGGAGATGATGTTATTGAAAAGGATTCCCCTGAATCACCATTTGAAGTAATTATTGAC AAAGCAGCATTTGACAAAGAATTTAAAGACTCATATAAGGAGAGCACAGATGATTTTGGTAGCTGGTCT GTGCACACTGATAAAGAATCATCCGAAGACATTTCAGAGACTAATGACAAGCTTTTTCCACTGAGAAAT AAAGAGGCAGGACGTTACCCAATGTCTGCATTGCTCAGTAGGCAGTTTTCACACACAAATGCAGCACTG GAAGAGGTGTCCAGATGCGTGAATGATATGCATAACTTTACTAACGAAATACTGACTTGGGATCTGGTT CCCCAAGTGAAACAACAGACCGATAAATCTTCTGACTGCATCACAAAAACTACAGGACTTGACATGAGT GAATATAATTCAGAAATTCCAGTTGTAAATCTTAAAACTAGCACTCATCAGAAAACTCCTGTATGTTCT ATTGATGGGAGCACTCCCATCACTAAATCAACAGGTGATTGGGCAGAAGCATCTCTCCAGCAAGAAAAT GCTATTACTGGAAAACCTGTACCTGACTCTTTGAATTCCACAAAAGAATTCAGTATCAAAGGTGTGCAA GGCAATATGCAGAAACAGGATGACACACTTGCAGAATTACCTGGATCTCCACCTGAGAAATGTGACTCT TTGGGTTCTGGAGTGGCCACAGTGAAAGTGGTTTTACCTGATGACCACCTGAAAGATGAAATGGACTGG CAGAGCTCTGCATTGGGAGAAATCACAGAAGCTGATAGTTCTGGTGAGTCTGATGACACAGTAATAGAG GACATCACAGCAGATACATCATTTGAAAATAACAAAATTCAGGCTGAAAAACCTGTTTCCATTCCAAGT GCTGTTGTAAAAACAGGTGAAAGAGAAATCAAAGAGATTCCCAGTTGTGAGAGAGAAGAAAAAACATCT AAAAACTTTGAAGAATTGGTCAGTGACTCTGAGCTGCATCAAGATCAGCCTGATATTCTTGGAAGGAGT CCAGCTAGTGAGGCAGCATGTTCAAAAGTACCCGATACGAATGTCTCCTTAGAAGATGTGAGTGAAGTT GCTCCTGAAAAGCCTATTACTACTGAGAACCCCAAACTTCCTTCAACAGTGTCTCCAAATGTTTTTAAT GAGACAGAATTCTCATTAAATGTGACAACATCTGCCTATTTGGAGTCATTACATGGGAAAAATGTTAAA CATATAGATGATTCCTCCCCAGAGGACCTGATAGCAGCCTTTACAGAAACCAGAGATAAAGGAATAGTA GATAGTGAAAGAAATGCTTTTAAAGCAATATCAGAGAAGATGACAGACTTTAAAACAACTCCTCCTGTA GAAGTCTTACATGAAAATGAGTCCGGTGGTTCTGAAATTAAAGACATTGGAAGCAAATACAGTGAACAA AGCAAAGAAACAAATGGAAGTGAGCCTCTAGGTGTTTTCCCTACCCAAGGTACTCCAGTAGCATCTCTT GACTTAGAACAAGAACAGCTCACAATTAAGGCTCTTAAAGAATTAGGTGAAAGACAGGTTGAGAAGTCA ACTTCTGCACAGCGTGACGCAGAATTGCCTTCTGAAGAAGTACTGAAGCAAACTTTCACATTTGCTCCA GAATCTTGGCCACAGAGATCATATGACATCCTAGAACGTAATGTCAAGAATGGATCTGATCTTGGGATT TCCCAGAAGCCCATCACTATCAGAGAAACTACTAGGGTAGATGCTGTTTCCAGCCTTAGCAAGACTGAA TTGGTAAAAAAGCATGTCCTAGCAAGACTTCTGACAGACTTCTCAGTGCACGATCTGATTTTCTGGAGA GATGTGAAGAAGACTGGGTTTGTCTTTGGCACCACGCTGATCATGCTGCTTTCCCTGGCAGCTTTCAGT GTCATCAGTGTGGTTTCTTACCTCATCCTGGCTCTTCTCTCTGTCACCATCAGCTTCAGGATCTACAAG TCCGTCATCCAAGCTGTACAGAAGTCAGAAGAAGGCCATCCATTCAAAGCCTACCTGGACGTAGACATT ACTCTGTCCTCAGAAGCTTTCCATAATTACATGAATGCTGCCATGGTGCACATCAACAGGGCCCTGAAA CTCATTATTCGTCTCTTTCTGGTAGAAGATCTGGTTGACTCCTTGAAGCTGGCTGTCTTCATGTGGCTG ATGACCTATGTTGGTGCTGTTTTTAACGGAATCACCCTTCTAATTCTTGCTGAACTGCTCATTTTCAGT GTCCCGATTGTCTATGAGAAGTACAAGACCCAGATTGATCACTATGTTGGCATCGCCCGAGATCAGACC AAGTCAATTGTTGAAAAGATCCAAGCAAAACTCCCTGGAATCGCCAAAAAAAAGGCAGAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001265589
Insert Size	3099 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001265589.1
RefSeq Size	4976 bp
RefSeq ORF	3099 bp
Locus ID	10313
UniProt ID	O95197
Protein Families	Transmembrane
MW	112.6 kDa
Gene Summary	This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12. [provided by RefSeq, May 2012] Transcript Variant: This variant (5) includes two consecutive alternate exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (e) is longer than isoform a.

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