EMR2 (ADGRE2) (NM_001271052) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD97; CD312; EMR2; VBU
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333030 representing NM_001271052. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACC CAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGC TGCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAAC GAGTGTGCAACACTGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGAGGGGAGCTAC GACTGCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAAC ACGTGTCAAGATGTGGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAAC ACCCTCGGCAGCTACACGTGCCAGTGCCTGCCTGGCTTCAAGCTCAAACCTGAGGACCCGAAGCTCTGC ACAGATGTGAATGAATGCACCTCCGGACAAAACCCATGCCACAGCTCCACCCACTGCCTCAACAACGTG GGCAGCTATCAGTGCCGCTGCCGCCCGGGCTGGCAACCGATTCCGGGGTCCCCCAATGGCCCAAACAAT ACCGTCTGTGAAGATGTGGACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTC AACACCGTGGGTTCATACAGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAAC CAAAAGGACACTGTCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAG ACGCTTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCCAATAAC ACCATCCAGAGCATCTTACAGGCGCTGGATGAGCTGCTGGAGGCCCCTGGGGACCTGGAGACCCTGCCC CGCTTACAGCAGCACTGTGTGGCCAGTCACCTGCTGGATGGCCTAGAGGATGTCCTCAGAGGCCTGAGC AAGAACCTTTCCAATGGGCTGTTGAACTTCAGTTATCCTGCAGGCACAGAATTGTCCCTGGAGGTGCAG AAGCAAGTAGACAGGAGTGTCACCTTGAGACAGAATCAGGCAGTGATGCAGCTCGACTGGAATCAGGCA CAGAAATCTGGTGACCCAGGCCCTTCTGTGGTGGGCCTTGTCTCCATTCCAGGGATGGGCAAGTTGCTG GCTGAGGCCCCTCTGGTCCTGGAACCTGAGAAGCAGATGCTTCTGCATGAGACACACCAGGGCTTGCTG CAGGACGGCTCCCCCATCCTGCTCTCAGATGTGATCTCTGCCTTTCTGAGCAACAACGACACCCAAAAC CTCAGCTCCCCAGTTACCTTCACCTTCTCCCACCGTGAGGAGGATCCCGTGCTGACTGTCATCACCTAC ATGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTCCTGTGTAAAGCCATC CAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTCCTGGCCCACCTCCTCTTCCTC GTGGCAATTGATCAAACCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCACTATCTCTAC CTGGCCACCTTGACCTGGATGCTGCTGGAGGCCCTGTACCTCTTCCTCACTGCACGGAACCTGACGGTG GTCAACTACTCAAGCATCAACAGATTCATGAAGAAGCTCATGTTCCCTGTGGGCTACGGAGTCCCAGCT GTGACAGTGGCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCCGCTGCTGGCTCCAA CCAGAAAAGGGATTTATATGGGGCTTCCTTGGACCTGTCTGCGCCATCTTCTCTGTGAATTTAGTTCTC TTTCTGGTGACTCTCTGGATTTTGAAAAACAGACTCTCCTCCCTCAATAGTGAAGTGTCCACCCTCCGG AACACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTGGGCTGCACGTGGTGTCTGGGC ATCTTGCAGGTGGGTCCGGCTGCCCGGGTCATGGCCTACCTCTTCACCATCATCAACAGCCTGCAGGGT GTCTTCATCTTCCTGGTGTACTGCCTCCTCAGCCAGCAGGTCCGGGAGCAATATGGGAAATGGTCCAAA GGGATCAGGAAATTGAAAACTGAGTCTGAGATGCACACACTCTCCAGCAGTGCTAAGGCTGACACCTCC AAACCCAGCACGGTTAACTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001271052
Insert Size	2298 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271052.1
RefSeq Size	6024 bp
RefSeq ORF	2298 bp
Locus ID	30817
UniProt ID	Q9UHX3
Protein Families	Druggable Genome, GPCR, Transmembrane
MW	84.1 kDa
Gene Summary	This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (8) differs in the 5' UTR and lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (h) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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