PPME1 (NM_001271593) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ABDH19; PME-1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333066 representing NM_001271593. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCGGCCCTCGAAAAGAGCATGCACCTCGGCCGCCTTCCCTCTCGCCCACCTCTACCCGGCAGCGGG GGCAGTCAGAGCGGAGCCAAGATGCGAATGGGCCCTGGAAGAAAGCGGGACTTTTCCCCTGTTCCTTGG AGTCAGTATTTTGAGTCCATGGAAGATGTAGAAGTAGAGAATGAAACTGGCAAGGATACTTTTCGAGTC TACAAGAGTGGTTCAGAGGGTCCAGTCCTGCTCCTTCTGCATGGAGGAGGTCATTCTGCCCTTTCTTGG GCTGTGTTCACGGCAGCGATTATTAGTAGAGTTCAGTGTAGGATTGTAGCTTTGGATCTGCGAAGTCAT GGTGAAACAAAGGTCAAGAATCCTGAAGATCTGTCTGCAGAAACAATGGCAAAAGACGTTGGCAATGTG GTTGAAGCCATGTATGGGGACCTTCCTCCTCCAATTATGCTGATTGGACATAGCATGGGTGGTGCTATT GCAGTCCACACAGCATCATCCAACCTGGTACCAAGCCTCTTGGGTCTGTGCATGATTGATGTTGTAGAA GGTACAGCTATGGATGCACTTAATAGCATGCAGAATTTCTTACGGGGTCGTCCTAAAACCTTCAAGTCT CTGGAGAATGCTATTGAATGGAGTGTGAAGAGTGGCCAGATTCGAAATCTGGAGTCTGCCCGTGTCTCA ATGGTTGGCCAAGTCAAACAGTGTGAAGGAATTACAAGTCCAGAAGGCTCAAAATCTATAGTGGAAGGA ATCATAGAGGAAGAAGAAGAAGATGAGGAAGGAAGTGAGTCTATAAGCAAGAGGAAAAAGGAAGATGAC ATGGAGGGGTTGCCCTCGGAGACTCAGAACTTGCTCTTATTCCTCCAGACCAAGAAAGACCATCCATAC ACCTGGAGAATTGAACTGGCAAAAACAGAAAAATACTGGGACGGCTGGTTCCGAGGCTTATCCAATCTC TTTCTTAGTTGTCCCATTCCTAAATTGCTGCTCTTGGCTGGTGTTGATAGATTGGATAAAGATCTGACC ATTGGCCAGATGCAAGGGAAGTTCCAGATGCAGGTCCTACCCCAGTGTGGCCATGCAGTCCATGAGGAT GCCCCTGACAAGGTAGCTGAAGCTGTTGCCACTTTCCTGATCCGGCACAGGTTTGCAGAACCCATCGGT GGATTCCAGTGTGTGTTTCCTGGCTGTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001271593
Insert Size	1203 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271593.1
RefSeq Size	2783 bp
RefSeq ORF	1203 bp
Locus ID	51400
UniProt ID	Q9Y570
Protein Families	Druggable Genome
MW	43.9 kDa
Gene Summary	This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. It encodes a longer protein (isoform b) compared to isoform a.

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