Prol1 (OPRPN) (NM_001302807) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BPLP; opiorphin; PRL1; PROL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC333750 representing NM_001302807. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAATTAACTTTCTTCTTGGGCCTGTTGGCTCTTATTTCATGTTTCACATCAGCGGTTGCTAAGCAT GAAGGGACAGTGGACTGCAGGAAGAGGACACAGGGCAGCCCAGTGAGAGTCAAAGATTCTCCAGAAGAC CATATCTACCTGGCCAGCTGCCACCACCTCCACTCTACAGGCCAAGATGGGTTCCACCAAGTCCCCCAC CTCCCTATGACTCAAGACTTAATTCACCACTTTCTCTTCCCTTTGTCCCAGGGCGAGTTCCACCATCTT CTTTCTCTCGATTTAGCCAAGCAGTCATTCTATCTCAACTCTTTCCATTGGAATCTATTAGACAACCTC GACTCTTTCCGGGTTATCCAAACCTACATTTCCCACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001302807
Insert Size	384 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302807.1
RefSeq Size	1116 bp
RefSeq ORF	384 bp
Locus ID	58503
Protein Families	Secreted Protein
MW	14.6 kDa
Gene Summary	This gene encodes a member of the proline-rich protein family. The encoded protein has multiple proposed functions, including roles in pain suppression, penile erection, and protection of the eye surface. The QRFSR pentapeptide, known as opiorphin, is derived from the N-terminal of this protein. Opiorphin inhibits the enkephalin-inactivating peptidases neprilysin and aminopeptidase N, and this activity is thought to reduce sensitivity to painful stimuli by effecting enkephalin-related activation of opioid-dependent pathways. Opiorphin may also act as an anti-depressant. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (2) contains an alternate exon in the 5' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. This isoform (2) lacks the functional QRFSR pentapeptide, known as opiorphin, located in the N-terminal of isoform 1.

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