RNF36 (TRIM69) (NM_001301145) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSD-34; HSD34; RNF36; Trif
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301145, the custom clone sequence may differ by one or more nucleotides ATGGAGTTTCTAAAGCTGCATCAGTTCCTGCACAGCAAAGAAAAGGACATTTTAACTGAGCTCCGGGAAG AGGGGAAAGCCTTGAATGAGGAGATGGAGTTGAATCTGAGCCAGCTTCAGGAGCAATGTCTCTTAGCCAA GGATATGTTGGACATCACAACTCTCTTACATAGCTTGGAGCAAGGAATGAAGGTGCTGGCAACCAGAGAG CTTATTTCCAGAAAGCTGAACCTGGGCCAGTACAAAGGTCCTATCCAGTACATGGTATGGAGGGAAATGC AGGACACTCTCTGCCCAGGCCTGTCTCCACTAACTCTGGACCCTAAAACAGCTCACCCAAATCTGGTGCT CTCCAAAAGCCAAACCAGCGTCTGGCATGGTGACATTAAGAAGATAATGCCTGATGATCCTGAGAGGTTT GACTCAAGTGTGGCTGTACTGGGCTCAAGAGGCTTCACCTCTGGAAAGTGGTACTGGGAAGTAGAAGTAG CAAAGAAGACAAAATGGACAGTTGGAGTTGTCAGAGAATCCATCATTCGGAAGGGCAGCTGTCCTCTAAC TCCTGAGCAAGGATTCTGGCTTTTAAGACTAAGGAACCAAACTGATCTAAAGGCTCTGGATTTGCCTTCT TTCAGTCTGACACTGACTAACAACCTCGACAAGGTGGGCATATACCTGGATTATGAAGGAGGACAGTTGT CCTTCTACAATGCTAAAACCATGACTCACATTTACACCTTCAGTAACACTTTCATGGAGAAACTTTATCC CTACTTCTGCCCCTGCCTTAATGATGGTGGAGAGAATAAAGAACCATTGCACATCTTACATCCACAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301145
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301145.1, NP_001288074.1
RefSeq Size	1394 bp
RefSeq ORF	840 bp
Locus ID	140691
UniProt ID	Q86WT6
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (d) lacks an alternate exon and uses two alternate splice sites, and it thus differs in its 5' UTR, initiates translation at a downstream in-frame start codon, and lacks an in-frame segment in the central coding region, compared to variant a. The encoded isoform (d, also known as TRIMLESS beta) is shorter at the N-terminus, compared to isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.