RNF36 (TRIM69) (NM_001301145) Human Tagged ORF Clone
CAT#: RC237128
- TrueORF®
TRIM69 (myc-DDK-tagged) - Human tripartite motif containing 69 (TRIM69), transcript variant d
ORF Plasmid: tGFP
"NM_001301145" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HSD-34; HSD34; RNF36; Trif |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237128 representing NM_001301145
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTTCTAAAGCTGCATCAGTTCCTGCACAGCAAAGAAAAGGACATTTTAACTGAGCTCCGGGAAG AGGGGAAAGCCTTGAATGAGGAGATGGAGTTGAATCTGAGCCAGCTTCAGGAGCAATGTCTCTTAGCCAA GGATATGTTGGACATCACAACTCTCTTACATAGCTTGGAGCAAGGAATGAAGGTGCTGGCAACCAGAGAG CTTATTTCCAGAAAGCTGAACCTGGGCCAGTACAAAGGTCCTATCCAGTACATGGTATGGAGGGAAATGC AGGACACTCTCTGCCCAGGCCTGTCTCCACTAACTCTGGACCCTAAAACAGCTCACCCAAATCTGGTGCT CTCCAAAAGCCAAACCAGCGTCTGGCATGGTGACATTAAGAAGATAATGCCTGATGATCCTGAGAGGTTT GACTCAAGTGTGGCTGTACTGGGCTCAAGAGGCTTCACCTCTGGAAAGTGGTACTGGGAAGTAGAAGTAG CAAAGAAGACAAAATGGACAGTTGGAGTTGTCAGAGAATCCATCATTCGGAAGGGCAGCTGTCCTCTAAC TCCTGAGCAAGGATTCTGGCTTTTAAGACTAAGGAACCAAACTGATCTAAAGGCTCTGGATTTGCCTTCT TTCAGTCTGACACTGACTAACAACCTCGACAAGGTGGGCATATACCTGGATTATGAAGGAGGACAGTTGT CCTTCTACAATGCTAAAACCATGACTCACATTTACACCTTCAGTAACACTTTCATGGAGAAACTTTATCC CTACTTCTGCCCCTGCCTTAATGATGGTGGAGAGAATAAAGAACCATTGCACATCTTACATCCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237128 representing NM_001301145
Red=Cloning site Green=Tags(s) MEFLKLHQFLHSKEKDILTELREEGKALNEEMELNLSQLQEQCLLAKDMLDITTLLHSLEQGMKVLATRE LISRKLNLGQYKGPIQYMVWREMQDTLCPGLSPLTLDPKTAHPNLVLSKSQTSVWHGDIKKIMPDDPERF DSSVAVLGSRGFTSGKWYWEVEVAKKTKWTVGVVRESIIRKGSCPLTPEQGFWLLRLRNQTDLKALDLPS FSLTLTNNLDKVGIYLDYEGGQLSFYNAKTMTHIYTFSNTFMEKLYPYFCPCLNDGGENKEPLHILHPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001301145 |
ORF Size | 837 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001301145.2 |
RefSeq Size | 1394 bp |
RefSeq ORF | 840 bp |
Locus ID | 140691 |
UniProt ID | Q86WT6 |
Protein Families | Druggable Genome |
MW | 32.5 kDa |
Gene Summary | This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |