CD300 (CD300LF) (NM_001289084) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD300f; CLM-1; CLM1; IgSF13; IREM-1; IREM1; LMIR3; NKIR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335234 representing NM_001289084. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCCTGCTGACACTCTACCTGCTCCTCTTCTGGCTCTCAGGCTACTCCATTGTCACTCAAATCACC GGTCCAACAACAGTGAATGGCTTGGAGCGGGGCTCCTTGACCGTGCAGTGTGTTTACAGATCAGGCTGG GAGACCTACTTGAAGTGGTGGTGTCGAGGAGCTATTTGGCGTGACTGCAAGATCCTTGTTAAAACCAGT GGGTCAGAGCAGGAGGTGAAGAGGGACCGGGTGTCCATCAAGGACAATCAGAAAAACCGCACGTTCACT GTGACCATGGAGGATCTCATGAAAACTGATGCTGACACTTACTGGTGTGGAATTGAGAAAACTGGAAAT GACCTTGGGGTCACAGTTCAAGTGACCATTGACCCAGCGTCGACTCCTGCCCCCACCACGCCTACCTCC ACTACGTTTACAGCACCAGTCACCCAAGAAGAAACTAGCAGCTCCCCAACTCTGACCGGCCACCACTTG GACAACAGGCACAAGCTCCTGAAGCTCAGTGTCCTCCTGCCCCTCATCTTCACCATATTGCTGCTGCTT TTGGTGGCCGCCTCACTCTTGGCTTGGAGGATGATGAAGTACCAGCAGAAAGCAGCCGGGATGTCCCCA GAGCAGGTACTGCAGCCCCTGGAGGGCGACCTCTGCTATGCAGACCTGACCCTGCAGCTGGCCGGAACC TCCCCGCAAAAGGCTACCACGAAGCTTTCCTCTGCCCAGGTTGACCAGGTGGAAGTGGAATATGTCACC ATGGCTTCCTTGCCGAAGGAGGACATTTCCTATGCATCTCTGACCTTGGGTGCTGAGGATCAGGAACCG ACCTACTGCAACATGGGCCACCTCAGTAGCCACCTCCCCGGCAGGGGCCCTGAGGAGCCCACGGAATAC AGCACCATCAGCAGGCCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289084
Insert Size	918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289084.1
RefSeq Size	1855 bp
RefSeq ORF	918 bp
Locus ID	146722
UniProt ID	Q8TDQ1
Protein Families	Druggable Genome, Transmembrane
MW	33.8 kDa
Gene Summary	This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (4) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (4) is longer, compared to isoform 1.

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