ZNF101 (NM_001300949) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HZF12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335294 representing NM_001300949. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAGCTCATGCTGGACACAAACGATCTGAGTGTGGTGGGGAATGGAGAGAGACGCCCCGTAAACAG AAACAACATGGGAAAGCCTCCATTTCCCCCAGTAGTGGTGCACGGCGCACAGTAACACCAACTCGAAAG AGACCTTATGAATGCAAGGTGTGCGGGAAAGCCTTTAATTCTCCCAATTTATTTCAAATCCATCAAAGA ACTCACACTGGAAAGAGGTCCTATAAATGTAGGGAAATAGTGAGAGCCTTCACAGTTTCCAGTTTCTTT CGAAAACATGGAAAAATGCATACTGGAGAAAAACGCTATGAATGTAAATACTGTGGAAAACCTATCGAT TATCCCAGTTTATTTCAAATTCATGTTAGAACTCACACTGGAGAAAAACCTTACAAATGTAAACAATGT GGTAAAGCCTTCATTTCCGCAGGTTACCTTCGGACACATGAAATCAGATCTCACGCGCTGGAGAAATCC CACCAATGTCAGGAATGTGGGAAAAAACTCAGTTGTTCCAGTTCCCTTCACAGACATGAAAGAACTCAT AGTGGAGGAAAACTCTACGAATGTCAAAAATGTGCCAAAGTCTTTAGATGTCCCACGTCCCTTCAAGCA CATGAAAGAGCTCACACTGGAGAAAGACCTTATGAATGTAATAAATGTGGTAAAACCTTCAATTATCCC AGTTGTTTTCGAAGACATAAAAAAACTCATAGTGGAGAAAAGCCATATGAATGTACAAGGTGTGGTAAA GCCTTTGGGTGGTGCAGTTCCCTCCGAAGACATGAAATGACTCACACTGGAGAAAAACCCTTTGATTGT AAACAGTGTGGTAAAGTCTTTACTTTTTCAAATTACCTTAGACTTCATGAAAGAACTCATTTGGCCGGG CGTAGCCAGTGCTTTGGCAGGAGGCAGGGGGATCACCTGAGCCCAGGAGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300949
Insert Size	951 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300949.1
RefSeq Size	4581 bp
RefSeq ORF	951 bp
Locus ID	94039
UniProt ID	Q8IZC7
Protein Families	Druggable Genome, Transcription Factors
MW	36.5 kDa
Gene Summary	Zinc finger proteins (ZNFs), such as ZNF101, bind nucleic acids and perform many key functions, the most important of which is regulating transcription (summary by Bellefroid et al., 1993 [PubMed 8467795]). See ZNF91 (MIM 603971) for general information on ZNFs.[supplied by OMIM, Nov 2010] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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