SLC35B2 (NM_001286513) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PAPST1; SLL; UGTrel4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335462 representing NM_001286513. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGCTGGCTATGCCAGCTTTATGGTACCTGGCTACCTCCTGGTGCAGTACTTCAGGCGGAAGAACT ACCTGGAGACCGGTGTCTTATCTGACTTGGGGTGTGCTGCAGGAAAGAGTGATGACCCGCAGCTATGGG GCCACAGCCACATCACCGGGTGAGCGCTTTACGGACTCGCAGTTCCTGGTGCTAATGAACCGAGTGCTG GCACTGATTGTGGCTGGCCTCTCCTGTGTTCTCTGCAAGCAGCCCCGGCATGGGGCACCCATGTACCGG TACTCCTTTGCCAGCCTGTCCAATGTGCTTAGCAGCTGGTGCCAATACGAAGCTCTTAAGTTCGTCAGC TTCCCCACCCAGGTGCTGGCCAAGGCCTCTAAGGTGATCCCTGTCATGCTGATGGGAAAGCTTGTGTCT CGGCGCAGCTACGAACACTGGGAGTACCTGACAGCCACCCTCATCTCCATTGGGGTCAGCATGTTTCTG CTATCCAGCGGACCAGAGCCCCGCAGCTCCCCAGCCACCACACTCTCAGGCCTCATCTTACTGGCAGGT TATATTGCTTTTGACAGCTTCACCTCAAACTGGCAGGATGCCCTGTTTGCCTATAAGATGTCATCGGTG CAGATGATGTTTGGGGTCAATTTCTTCTCCTGCCTCTTCACAGTGGGCTCACTGCTAGAACAGGGGGCC CTACTGGAGGGAACCCGCTTCATGGGGCGACACAGTGAGTTTGCTGCCCATGCCCTGCTACTCTCCATC TGCTCCGCATGTGGCCAGCTCTTCATCTTTTACACCATTGGGCAGTTTGGGGCTGCCGTCTTCACCATC ATCATGACCCTCCGCCAGGCCTTTGCCATCCTTCTTTCCTGCCTTCTCTATGGCCACACTGTCACTGTG GTGGGAGGGCTGGGGGTGGCTGTGGTCTTTGCTGCCCTCCTGCTCAGAGTCTACGCGCGGGGCCGTCTA AAGCAACGGGGAAAGAAGGCTGTGCCTGTTGAGTCTCCTGTGCAGAAGGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286513
Insert Size	1020 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286513.1
RefSeq Size	1929 bp
RefSeq ORF	1020 bp
Locus ID	347734
UniProt ID	Q8TB61
Protein Families	Druggable Genome, Transmembrane
MW	37.2 kDa
Gene Summary	Sulfotransferases (e.g., SULT4A1; MIM 608359) use an activated form of sulfate, 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS), as a common sulfate donor for sulfation of glycoproteins, proteoglycans, and glycolipids in the endoplasmic reticulum and Golgi apparatus. SLC35B2 is located in the microsomal membrane and transports PAPS from the cytosol, where it is synthesized, into the Golgi lumen (Kamiyama et al., 2003 [PubMed 12716889]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (6) lacks an alternate exon, resulting in a frameshifted 5' coding region and translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a.

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