HTRA3 (NM_001297559) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Prsp; Tasp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335587 representing NM_001297559. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGGCGCGAGCGCTGCTCCTGGCCGCGTTGGCCGCGCTGGCGCTGGCCCGGGAGCCCCCTGCGGCG CCGTGTCCCGCGCGCTGCGACGTGTCGCGGTGTCCCAGCCCCCGCTGCCCCGGCGGCTACGTGCCCGAC CTCTGCAACTGCTGCCTGGTGTGCGCCGCCAGCGAGGGCGAGCCCTGTGGCGGCCCTCTGGACTCGCCT TGCGGCGAGAGCCTGGAGTGCGTGCGCGGCCTATGCCGCTGCCGCTGGTCGCACGCCGTGTGTGGCACC GACGGGCACACCTATGCCAACGTGTGCGCGCTGCAGGCGGCCAGCCGCCGCGCGCTGCAGCTCTCCGGG ACGCCCGTGCGCCAGCTGCAGAAGGGCGCCTGCCCGTTGGGTCTCCACCAGCTGAGCAGCCCGCGCTAC AAGTTCAACTTCATTGCTGACGTGGTGGAGAAGATCGCACCAGCCGTGGTCCACATAGAGCTCTTCCTG AGACACCCGCTGTTTGGCCGCAACGTGCCCCTGTCCAGCGGTTCTGGCTTCATCATGTCAGAGGCCGGC CTGATCATCACCAATGCCCACGTGGTGTCCAGCAACAGTGCTGCCCCGGGCAGGCAGCAGCTCAAGGTG CAGCTACAGAATGGGGACTCCTATGAGGCCACCATCAAAGACATCGACAAGAAGTCGGACATTGCCACC ATCAAGATCCATCCCAAGAAAAAGCTCCCTGTGTTGTTGCTGGGTCACTCGGCCGACCTGCGGCCTGGG GAGTTTGTGGTGGCCATCGGCAGTCCCTTCGCCCTACAGAACACAGTGACAACGGGCATCGTCAGCACT GCCCAGCGGGAGGGCAGGGAGCTGGGCCTCCGGGACTCCGACATGGACTACATCCAGACGGATGCCATC ATCAACTACGGGAACTCCGGGGGACCACTGGTGAACCTGGATGGCGAGGTCATTGGCATCAACACGCTC AAGGTCACGGCTGGCATCTCCTTTGCCATCCCCTCAGACCGCATCACACGGTTCCTCACAGAGTTCCAA GACAAGCAGATCAAAGCCCCCTCACTGGCAGTTCATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297559
Insert Size	1074 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297559.1
RefSeq Size	2102 bp
RefSeq ORF	1074 bp
Locus ID	94031
UniProt ID	P83110
Protein Families	Druggable Genome, Protease, Secreted Protein
MW	37.9 kDa
Gene Summary	Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks several exons, and contains an alternate 3'-terminal exon, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus.

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