PHF23 (NM_001284518) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hJUNE-1b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335864 representing NM_001284518. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCGGTGATTGTCGGAGAGATCCACCTCCGACCCTTAAGCCAGAGACTCAGCCACCAGAGAAACGG CGGAGAACAATTGAGGATTTCAACAAATTCTGCAGTTTTGTTTTGGCATATGCTGGTTACATTCCCCCT AGCAAAGAGGAAAGTGACTGGCCAGCCTCTGGCTCCAGCTCTCCATTGCGAGGAGAGAGTGCGGCCGAC AGTGATGGCTGGGACTCGGCCCCCTCAGATCTTCGAACCATCCAGACTTTTGTTAAGAAAGCAAAGTCA TCCAAGAGAAGGGCAGCTCAAGCAGGTCCCACCCAGCCAGGACCCCCAAGGTCCACTTTCTCTCGTCTG CAGGCCCCCGACAGTGCTACCTTGCTTGAGAAGATGAAGCTCAAGGACTCTCTCTTTGATCTGGATGGG CCCAAAGTGGCATCTCCTTTGTCCCCCACATCCCTGACACATACCTCCCGGCCCCCTGCTGCTCTTACC CCCGTGCCCCTTTCCCAGGGGGACCTCTCCCATCCTCCTCGAAAGAAGGACCGAAAGAACCGAAAGTTG GGGCCAGGAGCTGGGGCTGGCTTTGGGGTGCTTCGGAGGCCTCGGCCAACTCCTGGGGATGGGGAAAAG AGATCTCGAATCAAGAAGAGCAAGAAGCGGAAGTTAAAAAAGGCAGAACGGGGGGATAGACTCCCACCT CCTGGGCCTCCCCAGGCACCCCCCAGTGATACAGACTCTGAAGAGGAGGAGGAAGAGGAGGAAGAGGAA GAAGAAGAAGAGATGGCAACAGTGGTAGGGGGTGAAGCCCCAGTCCCTGTGCTGCCAACACCCCCTGAG GCTCCTAGGCCCCCTGCCACAGTGCACCCTGAAGGAGTCCCTCCTGCTGACAGTGAAAGCAAGGAGGTG GGCAGCACTGAAACAAGCCAAGATGGAGATGCCAGCTCCAGTGAAGGCGAGATGCGGGTCATGGACGAG GACATCATGGTAGAATCAGGTGATGACTCATGGGATCTGATCACATGTTACTGTCGAAAGCCCTTTGCA GGGCGGCCCATGATTGAGTGCAGCCTGTGTGGGACGTGGATCCACCTCTCCTGTGCTAAGATTAAGAAG ACCAACGTCCCCGACTTCTTTTATTGCCAGAAATGCAAGGAACTGAGGCCAGAGGCCCGGCGGTTAGGG GGGCCTCCCAAATCTGGAGAGCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001284518
Insert Size	1200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284518.1
RefSeq Size	1938 bp
RefSeq ORF	1200 bp
Locus ID	79142
UniProt ID	Q9BUL5
Protein Families	Druggable Genome, Transcription Factors
MW	43.4 kDa
Gene Summary	Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate 5' exon and uses an alternate start codon compared to variant 1. It encodes isoform 3 which has a shorter and distinct N-terminus compared to isoform 1.

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