RUSC1 (NM_001278227) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NESCA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335872 representing NM_001278227. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGAAGCCCAGAGTGGGACTGGTCAGCTGCAGGAGCAGAAGAAAGGTCTTCTGATAGCCGTCAGC GTCTCCGTTGATAAAATCATCTCGCATTTCGGGGCCGCCCGGAACTTGGTGCAGAAGCCTTTCCGGAAG GACCTCATCACCGGGCAGCGCAGGAGCAGCCCCTGGAGCGTGGTGGAGGCGTCGGTGAAGCCAGGCTCC AGCACCCGCTCCCTTGGAACCCTGTATAGCCAGGTCAGCCGTCTAGCCCCGCTGAGCAGCAGCCGTAGC CGCTTCCATGCCTTTATCCTGGGCCTCCTCAACACCAAGCAGTTGGAGCTGTGGTTTTCCAGTCTCCAG GAAGATGCAGGCCTGCTCTCCCTCCTGTACCTGCCAACAGGATTTTTCTCCCTGGCCCGCGGTGGTTGT CCCTCCCTGTCCACAGAGCTGCTGCTCCTGCTGCAGCCATTGTCGGTGCTCACTTTCCACCTGGACCTG CTCTTTGAGCACCACCACCACCTGCCCCTGGGCCCACCTCAGGCCCCTGCCCCTCCAGGCCCACCTCCA GCTCTGCAGCAGACTATGCAAGCCATGCTGCACTTTGGGGGCCGGCTGGCCCAGAGCCTTCGGGGGACT TCCAAGGAAGCTGCTTCAGACCCCTCTGACTCTCCAAACCTTCCCACACCAGGGAGCTGGTGGGAGCAG TTGACCCAGGCCTCCCGGGTCTATGCCTCTGGGGGCACTGAGGGCTTTCCTCTTTCCCGATGGGCACCG GGGCGTCATGGGACTGCAGCTGAAGAAGGTGCACAGGAGAGACCCCTGCCCACAGATGAGATGGCACCA GGCAGGGGCCTCTGGTTGGGAAGACTATTTGGAGTGCCTGGGGGCCCCGCAGAAAATGAGAATGGAGCC CTAAAGTCCAGGAGACCATCTAGCTGGCTGCCCCCGACAGTGAGTGTGTTGGCTCTTGTGAAGCGGGGG GCACCTCCCGAGATGCCTTCTCCTCAGGAGCTTGAGGCCTCAGCACCCAGGATGGTGCAAACCCATAGG GCAGTGCGGGCTCTCTGTGATCACACTGCTGCAAGACCTGACCAGTTGAGCTTCCGGCGTGGGGAAGTG CTGCGTGTCATCACCACAGTGGATGAGGACTGGCTCCGCTGTGGGCGGGATGGCATGGAGGGTCTGGTG CCTGTGGGGTATACCTCCCTTGTTCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278227
Insert Size	1203 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278227.1
RefSeq Size	2469 bp
RefSeq ORF	1203 bp
Locus ID	23623
UniProt ID	Q9BVN2
MW	43.2 kDa
Gene Summary	Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR and contains multiple differences in the coding region, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (e) is shorter and has a shorter N-terminus, compared to isoform a.

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