DOC2A (NM_001282063) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Doc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335879 representing NM_001282063. Blue=Insert sequence Red=Cloning site Green=Tag(s) GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC ATGAGGGGCCGCAGGGGCGATCGCATGACCATCAACATCCAGGAGCACATGGCCATCAACGTGTGCCCC GGGCCCATCCGGCCCATCCGCCAGATCTCTGACTACTTCCCCCGGGGACCAGGACCTGAAGGGGGCGGC GGGGGCGGCGGGGAGGCCCCCGCCCATCTGGTCCCCCTGGCTCTGGCCCCCCCTGCAGCCCTCCTTGGG GCCACCACGCCTGAGGATGGTGCGGAGGTGGACAGCTATGACTCGGATGATGCCACCGCCCTAGGCACG CTGGAGTTTGACCTTCTCTACGACCGGGCCTCCTGCACTCTGCACTGTAGCATCCTCAGGGCCAAGGGC CTCAAGCCCATGGATTTCAATGGCCTCGCCGACCCCTACGTCAAGCTGCACTTGCTGCCTGGAGCCTGT AAGGCCAATAAGCTAAAAACGAAGACTCAGAGGAACACACTGAATCCCGTGTGGAATGAGGACCTGACT TACAGCGGGATCACAGATGACGACATCACGCACAAGGTGCTCAGGATCGCCGTCTGTGATGAGGACAAG CTGAGTCACAATGAGTTTATTGGGGAGATCCGCGTGCCCCTCCGCCGCCTCAAGCCTTCGCAGAAGAAG CATTTTAACATCTGCCTCGAGCGCCAGGTCCCGCTGGCGTCCCCCTCTTCCATGTCAGCGGCGCTGAGG GGCATCTCCTGTTATCTGAAGGAGTTGGAGCAGGCGGAGCAGGGGCAGGGGCTGCTGGAGGAGCGTGGC CGCATCCTGCTGAGTCTCAGCTACAGCTCGCGGCGCCGGGGACTGCTGGTAGGCATCTTGCGCTGCGCC CATCTGGCTGCCATGGACGTCAACGGTTACTCGGACCCCTACGTCAAGACGTACCTGAGGCCCGATGTG GACAAGAAATCCAAGCATAAGACGTGTGTGAAGAAGAAGACTCTCAACCCAGAATTTAACGAGGAGTTT TTCTACGAGATAGAGCTCTCCACTCTGGCCACCAAGACCCTGGAAGTCACCGTCTGGGACTATGACATT GGCAAATCCAATGACTTCATTGGTGGCGTGTCCCTGGGGCCAGGTGCCCGAGGCGAGGCTCGGAAGCAC TGGAGTGACTGCCTGCAGCAGCCGGACGCAGCCCTGGAGCGCTGGCACACCCTGACCAGTGAGCTGCCC CCTGCGGCCGGGGCTCTGTCCTCAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	AscI-MluI
ACCN	NM_001282063
Insert Size	1203 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282063.1
RefSeq Size	2090 bp
RefSeq ORF	1203 bp
Locus ID	8448
UniProt ID	Q14183
Protein Families	Secreted Protein
MW	44 kDa
Gene Summary	There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (2) contains an alternate terminal exon in the 5' UTR compared to variant 1. Variants 1 through 4 encode the same protein.

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