XRN1 (NM_001282859) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SEP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336244 representing NM_001282859. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAGTCCCCAAGTTTTACAGATGGATCTCAGAGCGGTATCCCTGTCTCAGCGAAGTGGTGAAAGAG CATCAGATTCCTGAATTTGACAACTTGTACCTGGATATGAATGGAATTATACATCAGTGCTCCCATCCT AATGATGATGATGTTCACTTTAGAATTTCAGATGATAAAATCTTTACTGATATTTTTCACTACCTGGAG GTGTTGTTTCGCATTATTAAACCCAGGAAAGTGTTCTTTATGGCTGTAGATGGTGTGGCTCCTCGAGCA AAAATGAACCAGCAGCGTGGGAGGCGTTTTAGGTCAGCAAAGGAGGCAGAAGACAAAATTAAAAAGGCA ATAGAGAAGGGAGAAACTCTTCCTACAGAGGCCAGATTTGATTCCAACTGTATCACACCAGGAACTGAA TTTATGGCCAGGTTACATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAGTCATGG CAAGGAGTTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAAATCATGGAATTT ATCAGATCCGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACACTGTCTTTATGGTTTAGATGCT GACTTGATTATGCTTGGATTAACAAGTCATGAGGCACATTTTTCTCTCTTAAGAGAAGAAGTTCGATTT GGTGGCAAAAAAACACAACGGGTATGTGCTCCAGAAGAAACTACATTTCACCTTCTACACTTGTCTTTA ATGAGAGAGTATATTGACTATGAGTTTTCAGTATTAAAAGAAAAGATCACATTTAAATATGATATTGAA AGGATAATAGATGATTGGATTTTGATGGGGTTTCTTGTTGGTAATGATTTTATCCCTCATCTACCTCAT TTACATATTAATCATGATGCACTGCCTCTTCTTTATGGAACATATGTTACCATCCTGCCAGAACTTGGG GGTTATATTAATGAAAGTGGGCACCTCAACTTACCTCGATTTGAGAAATACCTTGTGAAACTATCAGAT TTTGATCGGGAGCACTTCAGTGAAGTTTTTGTGGACCTAAAATGGTTTGAAAGCAAAGTTGGTAACAAG TACCTCAATGAAGCAGCAGGTGTCGCAGCAGAAGAAGCCAGGAACTACAAGGAAAAGAAAAAGTTAAAG GGCCAGGAAAATTCTCTGTGTTGGACTGCTTTAGACAAAAATGAAGGCGAAATGATAACTTCTAAGGAT AATTTAGAAGATGAGACTGAAGATGATGACCTATTTGAAACTGAGTTTAGACAATATAAAAGAACATAT TACATGACGAAGATGGGGGTTGACGTAGTATCTGAGTACGTTTTTGCCAATGCTTTTATTTTAAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282859
Insert Size	1380 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282859.1
RefSeq Size	2152 bp
RefSeq ORF	1380 bp
Locus ID	54464
UniProt ID	Q8IZH2
Protein Pathways	RNA degradation
MW	53.8 kDa
Gene Summary	This gene encodes a member of the 5'-3' exonuclease family. The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (4) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (d) has a shorter and distinct C-terminus, compared to isoform a.

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