PP5 (PPP5C) (NM_001204284) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PP5; PPP5; PPT
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336351 representing NM_001204284. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGATGGCGGAGGGCGAGAGGACTGAGTGTGCTGAGCCCCCCCGGGACGAACCCCCGGCTGATGGA GCTCTGAAGCGGGCAGAGGAGCTCAAGACTCAGGCCAATGACTACTTCAAAGCCAAGGACTACGAGAAC GCCATCAAGTTCTACAGCCAGGCCATCGAGCTGAACCCCAGCAATGCCATCTACTATGGCAACCGCAGC CTGGCCTACCTGCGCACTGAGTGCTATGGCTACGCGCTGGGAGACGCCACGCGGGCCATTGAGCTGGAC AAGAAGTACATCAAGGGTTATTACCGCCGGGCTGCCAGCAACATGGCACTGGGCAAGTTCCGGGCCGCG CTGCGAGACTACGAGACGGTGGTCAAGGTGAAGCCCCATGACAAGGATGCCAAAATGAAATACCAGGAG TGCAACAAGATCGTGAAGCAGAAGGCCTTTGAGCGGGCCATCGCGGGCGACGAGCACAAGCGCTCCGTG GTGGACTCGCTGGACATCGAGAGCATGACCATTGAGGATGAGTACAGCGGACCCAAGCTTGAAGACGGC AAAGTGACAATCAGTTTCATGAAGGAGCTCATGCAGTGGTACAAGGACCAGAAGAAACTGCACCGGAAA TGTGCCTACCAGACAGAGAAGATTACAGTATGTGGGGACACCCATGGCCAGTTCTATGACCTCCTCAAC ATATTCGAGCTCAACGGTTTACCCTCGGAGACCAACCCCTATATATTTAATGGTGACTTTGTGGACCGA GGCTCCTTCTCTGTAGAAGTGATCCTCACCCTTTTCGGCTTCAAGCTCCTGTACCCAGATCACTTTCAC CTCCTTCGAGGCAACCACGAGACAGACAACATGAACCAGATCTACGGTTTCGAGGGTGAGGTGAAGGCC AAGTACACAGCCCAGATGTACGAGCTCTTTAGCGAGGTGTTCGAGTGGCTCCCGTTGGCCCAGTGCATC AACGGCAAAGTGCTGATCATGCACGGAGGCCTGTTCAGTGAAGACGGTGTCACCCTGGATGACATCCGG AAAATTGAGCGGAATCGACAACCCCCAGATTCAGGGCCCATGTGTGACCTGCTCTGGTCAGATCCACAG CCACAGAACGGGCGCTCGATCAGCAAGCGGGGCGTGAGCTGTCAGTTTGGGCCTGACGTCACCAAGGCC TTCTTGGAAGAGAACAACCTGGACTATATCATCCGCAGCCACGAAGTCAAGGCCGAGGGCTACGAGGTG GCTCACGGAGGCCGCTGTGTCACCGTCTTCTCTGCCCCCAACTACTGCGACCAGATGGGGAACAAAGCC TCCTACATCCACCTCCAGGGCTCTGACCTACGGCCTCAGTTCCACCAGTTCACAGCAGTGCCTCATCCC AACGTCAAGCCCATGGCCTATGCCAACACGCTGCTGCAGCTAGGAATGATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001204284
Insert Size	1434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204284.1
RefSeq Size	2166 bp
RefSeq ORF	1434 bp
Locus ID	5536
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	MAPK signaling pathway
MW	54.4 kDa
Gene Summary	This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1.

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