beta IV Tubulin (TUBB4A) (NM_001289127) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	beta-5; DYT4; TUBB4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336415 representing NM_001289127. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAAGGGGGGCTGCGGACCGAGAAACTGAGGGACCGTGCTCCGCCGTCTCCGCCGCATCTTCCACC CTCGCCGCCGCCGCAGCTCCCCGCGCTCGTGCCACCGCCGCCGCGTCCACCCTCAGCGCCACCGCCATG CGGGAGATCGTGCACCTGCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTTTGGGAGGTTATC AGTGACGAACATGGCATCGACCCCACAGGCACATACCATGGGGACAGTGACCTGCAACTGGAGAGGATC AACGTGTACTACAACGAGGCCACAGGAGGAAATTATGTCCCCAGAGCGGTGCTGGTGGACCTGGAACCC GGCACCATGGACTCTGTCCGTTCTGGCCCCTTCGGTCAGATCTTTCGGCCGGACAACTTCGTGTTTGGC CAATCCGGAGCCGGCAACAACTGGGCAAAGGGGCACTACACGGAGGGCGCAGAGCTGGTGGACGCTGTC CTGGACGTAGTCCGGAAGGAGGCCGAGAGCTGCGACTGCCTTCAGGGCTTCCAGCTGACCCACTCGCTG GGGGGTGGCACGGGGTCCGGAATGGGCACGCTGCTCATCAGTAAGATCCGCGAGGAGTTCCCAGACCGC ATCATGAACACCTTCAGCGTGGTGCCCTCGCCCAAAGTGTCAGACACGGTGGTGGAGCCCTACAACGCC ACGCTGTCTGTGCACCAGCTGGTGGAGAATACGGATGAGACCTACTGCATCGACAACGAGGCACTCTAC GACATCTGTTTCCGCACCCTCAAGCTGACCACCCCCACCTACGGGGACCTCAACCACCTGGTGTCGGCC ACCATGAGCGGGGTCACCACCTGCCTGCGCTTCCCGGGCCAGCTGAACGCCGACCTGCGCAAGCTGGCC GTCAACATGGTTCCCTTTCCTCGCCTGCACTTCTTCATGCCCGGCTTCGCACCCCTGACCAGCCGGGGC AGCCAGCAGTACCGGGCCCTGACGGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATG GCGGCGTGCGACCCGCGCCACGGCCGCTACCTGACCGTGGCCGCCGTGTTCCGGGGCCGCATGTCCATG AAGGAGGTGGACGAGCAGATGCTGAGCGTGCAGAGCAAGAACAGCAGCTACTTCGTGGAGTGGATCCCC AACAACGTGAAGACGGCCGTGTGCGACATCCCGCCCCGCGGCCTGAAGATGGCCGCGACCTTCATCGGC AACAGCACGGCCATCCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAG GCCTTCTTGCACTGGTACACGGGCGAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATG AATGACCTGGTATCTGAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAGGGCGAGTTCGAGGAGGAG GCGGAGGAGGAGGTGGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289127
Insert Size	1470 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289127.1
RefSeq Size	2483 bp
RefSeq ORF	1470 bp
Locus ID	10382
UniProt ID	P04350
Protein Families	Druggable Genome
Protein Pathways	Gap junction, Pathogenic Escherichia coli infection
MW	53.8 kDa
Gene Summary	This gene encodes a member of the beta tubulin family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene cause hypomyelinating leukodystrophy-6 and autosomal dominant torsion dystonia-4. Alternate splicing results in multiple transcript variants encoding different isoforms. A pseudogene of this gene is found on chromosome X. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) has an alternate splice site in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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