Filensin (BFSP1) (NM_001278608) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CP94; CP115; CTRCT33; LIFL-H
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336616 representing NM_001278608. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTTGAACGGCTTAACAAGGAAGCTGATGAAGCCTTGCTGCATAACCTACGCCTTCAGCTGGAAGCC CAATTTCTGCAAGATGATATCAGTGCGGCAAAGGACAGGCACAAGAAGAATCTTCTGGAAGTTCAGACC TATATCAGCATCCTGCAGCAGATCATCCACACCACTCCTCCAGCATCCATTGTGACGAGTGGGATGAGG GAGGAGAAGCTCCTGACGGAGCGGGAGGTGGCCGCCCTGCGGAGTCAGCTGGAGGAGGGCCGGGAGGTG CTCTCCCACCTGCAGGCGCAGAGAGTGGAGCTGCAGGCACAGACAACAACTCTGGAACAAGCTATTAAA AGTGCCCATGAGTGTTATGACGATGAGATTCAGCTTTATAACGAGCAGATTGAGACACTGCGCAAGGAG ATTGAGGAGACAGAGCGGGTCCTGGAGAAGTCTTCTTACGACTGCCGGCAGCTGGCGGTCGCCCAGCAA ACCCTGAAGAATGAGCTGGACCGGTATCATCGTATCATCGAGATTGAAGGCAACAGGCTGACCTCTGCC TTCATTGAAACTCCCATTCCCCTGTTCACCCAGAGCCATGGAGTCTCTCTCAGCACTGGATCCGGTGGG AAAGATCTTACCAGAGCTCTGCAGGATATAACAGCAGCAAAACCAAGACAAAAAGCCCTCCCCAAGAAT GTTCCAAGGAGAAAAGAGATTATAACAAAAGACAAAACCAACGGAGCTCTGGAAGATGCACCATTAAAA GGTTTGGAAGACACAAAGCTGGTACAGGTGGTACTTAAAGAGGAAAGTGAATCTAAGTTTGAATCAGAA AGTAAAGAAGTAAGTCCCCTGACACAAGAAGGGGCTCCAGAGGATGTGCCAGATGGAGGGCAGATAAGC AAAGGCTTTGGGAAACTATACAGGAAGGTCAAGGAGAAAGTGAGAAGCCCCAAAGAGCCTGAGACCCCC ACTGAGCTCTACACCAAAGAGCGGCACGTGCTGGTCACAGGGGATGCCAATTACGTGGACCCTAGATTC TATGTCTCCTCCATCACAGCTAAAGGTGGGGTGGCTGTTTCTGTTGCGGAAGACTCTGTGCTTTATGAC GGCCAGGTGGAGCCCTCTCCTGAGTCACCCAAGCCCCCTTTAGAGAATGGGCAGGTGGGTCTGCAGGAG AAAGAAGATGGACAACCAATTGACCAGCAGCCTATAGACAAGGAGATTGAGCCAGATGGTGCAGAGCTG GAAGGCCCTGAAGAGAAACGTGAGGGTGAGGAGCGGGACGAAGAGTCCAGGAGACCCTGTGCCATGGTC ACACCCGGTGCAGAGGAACCATCTATACCTGAGCCTCCAAAGCCTGCGGCTGATCAGGATGGAGCTGAG GTGCTTGGGACTAGGAGCAGAAGCCTGCCAGAAAAAGGCCCTCCCAAGGCTTTGGCCTATAAGACAGTG GAAGTGGTGGAATCTATCGAGAAGATTTCCACGGAGAGCATTCAGACATATGAAGAAACCGCTGTGATC GTGGAGACCATGATTGGAAAGACAAAGTCAGACAAGAAGAAATCAGGAGAGAAGAGCTCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278608
Insert Size	1581 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278608.1
RefSeq Size	2198 bp
RefSeq ORF	1581 bp
Locus ID	631
UniProt ID	Q12934
MW	58.6 kDa
Gene Summary	This gene encodes a lens-specific intermediate filament-like protein named filensin. The encoded protein is expressed in lens fiber cells after differentiation has begun. This protein functions as a component of the beaded filament which is a cytoskeletal structure found in lens fiber cells. Mutations in this gene are the cause of autosomal recessive cortical juvenile-onset cataract. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (5) uses an alternate 5' structure and uses a downstream start codon, compared to isoform 1. The encodes isoform (3) has a shorter N-terminus compared to isoform 1. Variants 3 and 5 encode the same isoform (3).

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