RIC8 (RIC8A) (NM_001286134) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RIC8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336638 representing NM_001286134. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCCCCGGGCGGTTGCAGAAGCCGTGGAGACGGGTGAGGAGGATGTGATTATGGAAGCTCTGCGG TCATACAACCAGGAGCACTCCCAGAGCTTCACGTTTGATGATGCCCAACAGGAGGACCGGAAGAGACTG GCGGAGCTGCTGGTCTCCGTCCTGGAACAGGGCTTGCCACCCTCCCACCGTGTCATCTGGCTGCAGAGT GTCCGAATCCTGTCCCGGGACCGCAACTGCCTGGACCCGTTCACCAGCCGCCAGAGCCTGCAGGCACTA GCCTGCTATGCTGACATCTCTGTCTCTGAGGGGTCCGTCCCAGAGTCCGCAGACATGGATGTTGTACTG GAGTCCCTCAAGTGCCTGTGCAACCTCGTGCTCAGCAGCCCTGTGGCACAGATGCTGGCAGCAGAGGCC CGCCTAGTGGTGAAGCTCACAGAGCGTGTGGGGCTGTACCGTGAGAGGAGCTTCCCCCACGATGTCCAG TTCTTTGACTTGCGGCTCCTCTTCCTGCTAACGGCACTCCGCACCGATGTGCGCCAGCAGCTGTTTCAG GAGCTGAAAGGAGTGCGCCTGCTAACTGACACACTGGAGCTGACGCTGGGGGTGACTCCTGAAGGGAAC CCCCCACCCACGCTCCTTCCTTCCCAAGAGACTGAGCGGGCCATGGAGATCCTCAAAGTGCTCTTCAAC ATCACCCTGGACTCCATCAAGGGGGAGGTGGACGAGGAAGACGCTGCCCTTTACCGACACCTGGGGACC CTTCTCCGGCACTGTGTGATGATCGCTACTGCTGGAGACCGCACAGAGGAGTTCCAGGGCCACGCAGTG AACCTCCTGGGGAACTTGCCCCTCAAGTGTCTGGATGTTCTCCTCACCCTGGAGCCACATGGAGACTCC ACGGAGTTCATGGGAGTGAATATGGATGTGATTCGTGCCCTCCTCATCTTCCTAGAGAAGCGTTTGCAC AAGACACACAGGCTGAAGGAGAGTGTAGCTCCCGTGCTGAGCGTGCTGACTGAATGTGCCCGGATGCAC CGCCCAGCCAGGAAGTTCCTGAAGGCCCAGGTGCTGCCCCCTCTGCGGGATGTGAGGACACGGCCTGAG GTTGGGGAGATGCTGCGGAACAAGCTTGTCCGCCTCATGACACACCTGGACACAGATGTGAAGAGGGTG GCTGCCGAGTTCTTGTTTGTCCTGTGCTCTGAGAGTGTGCCCCGATTCATCAAGTACACAGGCTATGGG AATGCTGCTGGCCTTCTGGCTGCCAGGGGCCTCATGGCAGGAGGCCGGCCCGAGGGCCAGTACTCAGAG GATGAGGACACAGACACAGATGAGTACAAGGAAGCCAAAGCCAGCATAAACCCTGTGACCGGGAGGGTG GAGGAGAAGCCGCCTAACCCTATGGAGGGCATGACAGAGGAGCAGAAGGAGCACGAGGCCATGAAGCTG GTGACCATGTTTGACAAGCTCTCCAGGAACAGAGTCATCCAGCCAATGGGGATGAGTCCCCGGGGTCAT CTTACGTCCCTGCAGGATGCCATGTGCGAGACTATGGAGCAGCAGCTCTCCTCGGACCCTGACTCGGAC CCTGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI      Plasmid Map
ACCN	NM_001286134
Insert Size	1596 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286134.1
RefSeq Size	3765 bp
RefSeq ORF	1596 bp
Locus ID	60626
UniProt ID	Q9NPQ8
MW	59.7 kDa
Gene Summary	Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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