VPS45A (VPS45) (NM_001279353) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A; VSP45; VSP45A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336687 representing NM_001279353. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTATACACACAATCGGAGATTCTACAGAAGGAAGTGTACCTCTTTGAACGCATTGATTCTCAAAAT CGAGAGATCATGAAACACCTGAAGGCAATTTGTTTTCTTCGACCTACAAAGGAGAATGTGGATTATATT ATTCAGGAGCTCCGAAGACCCAAATACACTATATATTTCATTTATTTCAGTAATGTGATCAGCAAGAGT GACGTGAAGTCATTGGCTGAAGCTGATGAACAGGAAGTTGTGGCTGAGGTTCAGCAAGTGATAACTAAA GAATATGAACTGTTTGAATTCCGTCGGACAGAGGTTCCTCCATTGCTCCTTATTTTAGATCGCTGTGAT GATGCCATCACCCCATTGCTAAACCAGTGGACATATCAGGCCATGGTCCACGAACTACTAGGCATAAAC AACAATCGGATTGATCTTTCCAGAGTGCCGGGAATCAGTAAAGACTTAAGAGAAGTGGTCCTATCTGCT GAAAATGATGAATTCTATGCTAATAATATGTACCTGAACTTTGCTGAGATTGGTAGCAATATAAAGAAT CTCATGGAAGATTTTCAGAAGAAGAAACCAAAAGAACAGCAAAAACTAGAATCAATAGCAGACATGAAG GCGTTTGTTGAGAATTATCCACAGTTCAAGAAAATGTCTGGGACTGTTTCAAAGCATGTGACAGTGGTT GGAGAACTGTCTCGATTGGTCAGTGAACGGAATCTGCTGGAGGTTTCAGAGGTTGAGCAAGAACTGGCC TGTCAAAATGACCATTCTAGTGCTCTCCAGAATATAAAAAGGCTTCTGCAGAACCCCAAAGTGACAGAG TTTGATGCTGCCCGCCTGGTGATGCTTTATGCTTTACATTATGAGCGACACAGCAGCAATAGCCTGCCA GGACTAATGATGGACCTCAGGAATAAAGGTGTTTCTGAGAAGTATCGAAAGCTCGTGTCTGCAGTTGTT GAATATGGTGGTAAACGAGTCAGAGGAAGTGACCTCTTCAGCCCCAAAGATGCTGTGGCTATCACCAAA CAATTCCTCAAAGGACTGAAGGGAGTAGAAAATGTATATACACAGCATCAACCTTTCCTACATGAAACC CTGGATCATCTCATCAAAGGAAGGCTTAAGGAAAACCTATATCCTTATTTAGGCCCCAGCACACTCAGA GACAGACCTCAGGATATCATTGTGTTTGTAATTGGAGGAGCCACCTATGAAGAGGCTCTAACAGTTTAT AACCTGAACCGCACCACTCCTGGAGTGAGGATTGTCCTGGGAGGCACCACAGTGCACAACACGAAAAGA GATGGGGTTTCACTATGTTCCCCTGCCTGGTTTCGAACTCCTGGGCTCAAGCGATCCACCCGCCTCAGC CTCCCAAAGTGCTGGGATTACAGCTTTCCTAGAGGAAGTTCTGGCTTCTGGACTGCACAGCCGAAGCAA GGAGAGCTCTCAAGTCACATCAAGGTCAGCGAGCAGAAGATGAAACGGTGGTTGGGGGAAGGGCACAGC TTCCTCTCTTGTCCCCACTACAGGTTTTCCCTACTAAACAAAGGTGTTGGAGAGCAGCTTTGGGTTCTG TGCTGGTTGTTAGAACTCATCTCCAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001279353
Insert Size	1617 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001279353.1
RefSeq Size	2367 bp
RefSeq ORF	1617 bp
Locus ID	11311
UniProt ID	Q9NRW7
Protein Pathways	Endocytosis
MW	62 kDa
Gene Summary	Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) differs in the 5' UTR and has multiple differences in the coding region, compared to variant 1. The resulting isoform (2) is shorter and has a shorter N-terminus, lacks an internal segment and has a longer and distinct C-terminus, compared to isoform 1.

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