CRMP1 (NM_001288661) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CRMP-1; DPYSL1; DRP-1; DRP1; ULIP-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336825 representing NM_001288661. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGAAAATGAACAACGAAGTGGTGGACAAGAGTGACCGACTCCTCATCAAAGGTGGACGGATCATC AACGATGACCAATCCCTTTATGCTGACGTCTACCTGGAGGATGGACTTATCAAACAAATAGGAGAGAAC TTAATCGTTCCTGGTGGAGTGAAGACCATTGAAGCCAACGGGCGGATGGTTATTCCCGGAGGTATTGAT GTCAACACGTACCTGCAGAAGCCCTCCCAGGGGATGACTGCGGCTGATGACTTCTTCCAAGGGACCAGG GCGGCACTGGTGGGCGGGACCACGATGATCATTGACCATGTTGTTCCTGAACCTGGGTCCAGCCTACTG ACCTCTTTCGAGAAGTGGCACGAAGCAGCTGACACCAAATCCTGCTGTGATTACTCCCTCCACGTGGAC ATCACAAGCTGGTACGATGGCGTTCGGGAGGAGCTGGAGGTGCTGGTGCAGGACAAAGGCGTCAATTCC TTCCAAGTCTACATGGCCTATAAGGATGTCTACCAAATGTCCGACAGCCAGCTCTATGAAGCCTTTACC TTCCTTAAGGGCCTGGGAGCTGTGATCTTGGTCCATGCAGAAAATGGAGATTTGATAGCTCAGGAACAA AAGCGGATCCTGGAGATGGGCATCACGGGTCCCGAGGGCCATGCCCTGAGCAGACCTGAAGAGCTGGAG GCCGAGGCGGTGTTCCGGGCCATCACCATTGCGGGCCGGATCAACTGCCCTGTGTACATCACCAAGGTC ATGAGCAAGAGTGCAGCCGACATCATCGCTCTGGCCAGGAAGAAAGGGCCCCTAGTTTTTGGAGAGCCC ATTGCCGCCAGCCTGGGGACCGATGGCACCCATTACTGGAGCAAGAACTGGGCCAAGGCTGCGGCGTTC GTGACTTCCCCTCCCCTGAGCCCGGACCCTACCACGCCCGACTACTTGACCTCCCTACTGGCCTGTGGG GACTTGCAGGTCACAGGCAGCGGCCACTGTCCCTACAGCACTGCCCAGAAGGCGGTGGGCAAGGACAAC TTTACCCTGATCCCCGAGGGTGTCAACGGGATAGAGGAGCGGATGACGGTCGTCTGGGACAAGGCGGTG GCTACTGGCAAAATGGATGAGAACCAGTTTGTCGCTGTCACCAGCACCAATGCAGCCAAGATCTTTAAC CTGTACCCAAGGAAAGGGCGGATTGCCGTGGGCTCGGATGCCGACGTGGTCATCTGGGACCCCGACAAG TTGAAGACCATAACAGCCAAAAGTCACAAGTCGGCGGTGGAGTACAACATCTTCGAGGGTATGGAGTGC CACGGCTCCCCACTAGTGGTCATCAGCCAGGGCAAGATCGTCTTTGAAGACGGAAACATCAACGTCAAC AAGGGCATGGGCCGCTTCATTCCGCGGAAGGCGTTCCCGGAGCACCTGTACCAGCGCGTCAAAATCAGG AATAAGGTTTTTGGATTGCAAGGGGTTTCCAGGGGCATGTATGACGGTCCTGTGTACGAGGTACCAGCT ACACCCAAATATGCAACTCCCGCTCCTTCAGCCAAATCTTCGCCTTCTAAACACCAGCCCCCACCCATC AGAAACCTCCACCAGTCCAACTTCAGCTTATCAGGTGCCCAGATAGATGACAACAATCCCAGGCGCACC GGCCACCGCATCGTGGCGCCCCCTGGTGGCCGCTCCAACATCACCAGCCTCGGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288661
Insert Size	1713 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288661.1
RefSeq Size	3278 bp
RefSeq ORF	1713 bp
Locus ID	1400
UniProt ID	Q14194
MW	62 kDa
Gene Summary	This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) contains an alternate 5' exon, compared to variant 1. It differs in the 5' UTR and initiates translation at an alternate downstream AUG resulting in a protein (isoform 3) with a shorter N-terminus, compared to isoform 1.

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