RPS6KC1 (NM_001287220) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	humS6PKh1; RPK118; RSKL1; S6K-delta-1; S6PKh1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336943 representing NM_001287220. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTTAAAGCTCTGCCTTTGAAGAGTAGTCTTACTCCAAGTTCTCAAGATGACAGCAACCAGGAAGAT GATGGCCAAGATAGCTCTCCAAAGTGGCCAGATTCTGGTTCAAGTTCAGAAGAAGAATGTACTACTAGT TATTTAACATTATGCAATGAATATGGGCAAGAAAAGATTGAACCAGGGTCTTTGAATGAGGAGCCCTTC ATGAAGACTGAAGGGAATGGTGTTGATACAAAAGCTATTAAAAGCTTCCCAGCACACCTTGCTGCTGAC AGTGACAGCCCCAGCACACAGCTGAGAGCTCACGAGCTGAAGTTCTTCCCCAACGATGACCCAGAAGCA GTTAGTTCTCCAAGAACATCAGATTCCCTCAGTAGATCAAAAAATAGCCCCATGGAATTCTTTAGGATA GACAGTAAGGATAGCGCAAGTGAACTCCTGGGACTTGACTTTGGAGAAAAATTGTATAGTCTAAAATCA GAACCTTTGAAACCATTCTTTACTCTTCCAGATGGAGACAGTGCTTCTAGGAGTTTTAATACTAGTGAA AGCAAGGTAGAGTTTAAAGCTCAGGACACCATTAGCAGGGGCTCAGATGACTCAGTGCCAGTTATTTCA TTTAAAGATGCTGCTTTTGATGATGTCAGTGGTACTGATGAAGGAAGACCTGATCTTCTTGTAAATTTA CCTGGTGAATTGGAGTCAACAAGAGAAGCTGCAGCAATGGGACCTACTAAGTTTACACAAACTAATATA GGGATAATAGAAAATAAACTCTTGGAAGCCCCTGATGTTTTATGCCTCAGGCTTAGTACTGAACAATGC CAAGCACATGAGGAGAAAGGCATAGAGGAACTGAGTGATCCCTCTGGGCCCAAATCCTATAGTATAACA GAGAAACACTATGCACAGGAGGATCCCAGGATGTTATTTGTAGCAGCTGTTGATCATAGTAGTTCAGGA GATATGTCTTTGTTACCCAGCTCAGATCCTAAGTTTCAAGGACTTGGAGTGGTTGAGTCAGCAGTAACT GCAAACAACACAGAAGAAAGCTTATTCCGTATTTGTAGTCCACTCTCAGGTGCTAATGAATATATTGCA AGCACAGACACTTTAAAAACAGAAGAAGTATTGCTGTTTACAGATCAGACTGATGATTTGGCTAAAGAG GAACCAACTTCTTTATTCCAGAGAGACTCTGAGACTAAGGGTGAAAGTGGTTTAGTGCTAGAAGGAGAC AAGGAAATACATCAGATTTTTGAGGACCTTGATAAAAAATTAGCACTAGCCTCCAGGTTTTACATCCCA GAGGGCTGCATTCAAAGATGGGCAGCTGAAATGGTGGTAGCCCTTGATGCTTTACATAGAGAGGGAATT GTGTGCCGCGATTTGAACCCAAACAACATCTTATTGAATGATAGAGGACACATTCAGCTAACGTATTTT AGCAGGTGGAGTGAGGTTGAAGATTCCTGTGACAGCGATGCCATAGAGAGAATGTACTGTGCCCCAGAG GTTGGAGCAATCACTGAAGAAACTGAAGCCTGTGATTGGTGGAGTTTGGGTGCTGTCCTCTTTGAACTT CTCACTGGCAAGACTCTGGTTGAATGCCATCCAGCAGGAATAAATACTCACACTACTTTGAACATGCCA GAATGTGTCTCTGAAGAGGCTCGCTCACTCATTCAACAGCTCTTGCAGTTCAATCCTCTGGAACGACTT GGTGCTGGAGTTGCTGGTGTTGAAGATATCAAATCTCATCCATTTTTTACCCCTGTGGATTGGGCAGAA CTGATGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287220
Insert Size	1806 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287220.1
RefSeq Size	4038 bp
RefSeq ORF	1806 bp
Locus ID	26750
Protein Families	Druggable Genome, Protein Kinase
MW	66.1 kDa
Gene Summary	Sphingosine kinase catalyzes the formation of sphingosine 1 phosphate, a lipid cellular messenger. The protein encoded by this gene can bind to sphingosine kinase and to phosphatidylinositol 3-phosphate, suggesting a role in sphingosine 1 phophate signaling. The encoded protein can also bind to peroxiredoxin-3 and may help transport it to mitochondria. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (5) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (d) has a shorter N-terminus, compared to isoform a. Variants 5 and 22-30 all encode the same isoform (d). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.