ACSF2 (NM_001288969) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACSMW; AVYV493
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336946 representing NM_001288969. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGTCTACGTCGGGATGCTGCGCCTGGGGAGGCTGTGCGCCGGGAGCTCGGGGGTGCTGGGGGCC CGGGCCGCCCTCTCTCGGAGTTGGCAGGAAGCCAGGTTGCAGGGTGTCCGCTTCCTCAGTTCCAGAGAG GTGGATCGCATGGTCTCCACGCCCATCGGAGGCCTCAGCTACGTTCAGGGGTGCACCAAAAAGCATCTT AACAGCAAGACTGTGGGCCAGTGCCTGGAGACCACAGCACAGAGGGTCCCAGAACGAGAGGCCTTGGTC GTCCTCCATGAAGACGTCAGGTTGACCTTTGCCCAACTCAAGGAGGAGGTGGACAAAGCTGCTTCTGGC CTCCTGAGCATTGGCCTCTGCAAAGGTGACCGGCTGGGCATGTGGGGACCTAACTCCTATGCATGGGTG CTCATGCAGTTGGCCACCGCCCAGGCTATGGAACTGGAGTATGTCCTCAAGAAGGTGGGCTGCAAGGCC CTTGTGTTCCCCAAGCAATTCAAGACCCAGCAATACTACAACGTCCTGAAGCAGATCTGTCCAGAAGTG GAGAATGCCCAGCCAGGGGCCTTGAAGAGTCAGAGGCTCCCAGATCTGACCACAGTCATCTCGGTGGAT GCCCCTTTGCCGGGGACCCTGCTCCTGGATGAAGTGGTGGCGGCTGGCAGCACACGGCAGCATCTGGAC CAGCTCCAATACAACCAGCAGTTCCTGTCCTGCCATGACCCCATCAACATCCAGTTCACCTCGGGGACA ACAGGCAGCCCCAAGGGGGCCACCCTCTCCCACTACAACATTGTCAACAACTCCAACATTTTAGGAGAG CGCCTGAAACTGCATGAGAAGACACCAGAGCAGTTGCGGATGATCCTGCCCAACCCCCTGTACCATTGC CTGGGTTCCGTGGCAGGCACAATGATGTGTCTGATGTACGGTGCCACCCTCATCCTGGCCTCTCCCATC TTCAATGGCAAGAAGGCACTGGAGGCCATCAGCAGAGAGAGAGGCACCTTCCTGTATGGTACCCCCACG ATGTTCGTGGACATTCTGAACCAGCCAGACTTCTCCAGTTATGACATCTCGACCATGTGTGGAGGTGTC ATTGCTGGGTCCCCTGCACCTCCAGAGTTGATCCGAGCCATCATCAACAAGATAAATATGAAGGACCTG GTGGTTGCTTATGGAACCACAGAGAACAGTCCCGTGACATTCGCGCACTTCCCTGAGGACACTGTGGAG CAGAAGGCAGAAAGCGTGGGCAGAATTATGCCTCACACGGAGGCCCGGATCATGAACATGGAGGCAGGG ACGCTGGCAAAGCTGAACACGCCCGGGGAGCTGTGCATCCGAGGGTACTGCGTCATGCTGGGCTACTGG GGTGAGCCTCAGAAGACAGAGGAAGCAGTGGATCAGGACAAGTGGTATTGGACAGGAGATGTCGCCACA ATGAATGAGCAGGGCTTCTGCAAGATCGTGGGCCGCTCTAAGGATATGATCATCCGGGGTGGTGAGAAC ATCTACCCCGCAGAGCTCGAGGACTTCTTTCACACACACCCGAAGGTGCAGGAAGTGCAGGTGGTGGGA GTGAAGGACGATCGGATGGGGGAAGAGATTTGTGCCTGCATTCGGCTGAAGGACGGGGAGGAGACCACG GTGGAGGAGATAAAAGCTTTCTGCAAAGGGAAGATCTCTCACTTCAAGATTCCGAAGTACATCGTGTTT GTCACAAACTACCCCCTCACCATTTCAGGAAAGATCCAGAAATTCAAACTTCGAGAGCAGATGGAACGA CATCTAAATCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288969
Insert Size	1809 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288969.1
RefSeq Size	2206 bp
RefSeq ORF	1809 bp
Locus ID	80221
UniProt ID	Q96CM8
MW	66.8 kDa
Gene Summary	Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipocyte differentiation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region, and uses two alternate in-frame splice sites in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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