Pericentrin 1 (NUP85) (NM_001303276) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FROUNT; NPHS17; Nup75
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336972 representing NM_001303276. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGCCAAGTTGCCCCTTTATCTATATCATCCGTAAGGATGTAGATGTTTACTCTCAAATCTTGAGA AAACTCTTCAATGAATCCCATGGAATCTTTCTGGGCCTCCAGAGAATTGACGAAGAGTTGACTGGAAAA TCCAGAAAATCTCAATTGGTTCGAGTGAGTAAAAACTACCGATCAGTCATCAGAGCATGTATGGAGGAA ATGCACCAGGTTGCAATTGCTGCTAAAGATCCAGCCAATGGCCGCCAGTTCAGCAGCCAGGTCTCCATT TTGTCAGCAATGGAGCTCATCTGGAACCTGTGTGAGATTCTTTTTATTGAAGTGGCCCCAGCTGGCCCT CTCCTCCTCCATCTCCTTGACTGGGTCCGGCTCCATGTGTGCGAGGTGGACAGTTTGTCGGCAGATGTT CTGGGCAGTGAGAATCCAAGCAAACATGACAGCTTCTGGAACTTGGTGACCATCTTGGTGCTGCAGGGC CGGCTGGATGAGGCCCGACAGATGCTCTCCAAGGAAGCCGATGCCAGCCCCGCCTCTGCAGGCATATGC CGAATCATGGGGGACCTGATGAGGACAATGCCCATTCTTAGTCCCGGGAACACCCAGACACTGACAGAG CTGGAGCTGAAGTGGCAGCACTGGCACGAGGAATGTGAGCGGTACCTCCAGGACAGCACATTCGCCACC AGCCCTCACCTGGAGTCTCTCTTGAAGATTATGCTGGGAGACGAAGCTGCCTTGTTAGAGCAGAAGGAA CTTCTGAGTAATTGGTATCATTTCCTAGTGACTCGGCTCTTGTACTCCAATCCCACAGTAAAACCCATT GATCTGCACTACTATGCCCAGTCCAGCCTGGACCTGTTTCTGGGAGGTGAGAGCAGCCCAGAACCCCTG GACAACATCTTGTTGGCAGCCTTTGAGTTTGACATCCATCAAGTAATCAAAGAGTGCAGCATCGCCCTG AGCAACTGGTGGTTTGTGGCCCACCTGACAGACCTGCTGGACCACTGCAAGCTCCTCCAGTCACACAAC CTCTATTTCGGTTCCAACATGAGAGAGTTCCTCCTGCTGGAGTACGCCTCGGGACTGTTTGCTCATCCC AGCCTGTGGCAGCTGGGGGTCGATTACTTTGATTACTGCCCCGAGCTGGGCCGAGTCTCCCTGGAGCTG CACATTGAGCGGATACCTCTGAACACCGAGCAGAAAGCCCTGAAGGTGCTGCGGATCTGTGAGCAGCGG CAGATGACTGAACAAGTTCGCAGCATTTGTAAGATCTTAGCCATGAAAGCCGTCCGCAACAATCGCCTG GGTTCTGCCCTCTCTTGGAGCATCCGTGCTAAGGATGCCGCCTTTGCCACGCTCGTGTCAGACAGGTTC CTCAGGGATTACTGTGAGCGAGGCTGCTTTTCTGATTTGGATCTCATTGACAACCTGGGGCCAGCCATG ATGCTCAGTGACCGACTGACATTCCTGGGAAAGTATCGCGAGTTCCACCGTATGTACGGGGAGAAGCGT TTTGCCGACGCAGCTTCTCTCCTTCTGTCCTTGATGACGTCTCGGATTGCCCCTCGGTCTTTCTGGATG ACTCTGCTGACAGACGCCTTGCCCCTTTTGGAACAGAAACAGGTGATTTTCTCAGCAGAACAGACTTAT GAGTTGATGCGGTGTCTGGAGGACTTGACGTCAAGAAGACCTGTGCATGGAGAATCTGATACCGAGCAG CTCCAGGATGATGACATAGAGACCACCAAGGTGGAAATGCTGAGACTTTCTCTGGCACGAAATCTTGCT CGGGCAATTATAAGAGAAGGCTCACTGGAAGGTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303276
Insert Size	1833 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303276.1
RefSeq Size	2285 bp
RefSeq ORF	1833 bp
Locus ID	79902
UniProt ID	Q9BW27
MW	69.8 kDa
Gene Summary	This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (2) lacks an alternate exon in the 5' region and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.

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