EXOC7 (NM_001282313) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2-5-3p; BLOM4; EX070; EXO70; Exo70p; EXOC1; NEDSEBA; YJL085W
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337123 representing NM_001282313. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGTCTATCTTATCATCCTTTGAGAGCCGCCTTATGAAGCTGGAGAACTCCATCATCCCTGTGCAC AAGCAGACGGAGAATCTGCAGCGGCTGCAGGAGAATGTTGAGAAGACGCTGTCCTGCCTGGACCATGTC ATCAGCTACTACCATGTGGCCAGTGACACTGAGAAGATCATCAGAGAGGGCCCCACAGGTAGGCTGGAA GAGTACCTGGGAAGCATGGCCAAGATTCAGAAGGCAGTGGAGTATTTCCAGGACAACAGCCCAGACAGC CCGGAACTCAACAAAGTGAAACTGCTCTTTGAGCGCGGGAAGGAGGCCCTGGAGTCCGAATTTCGCAGC CTGATGACGCGGCACAGTAAGGTCGTCTCGCCCGTGCTCATCTTGGATCTGATCAGTGGTGACGATGAT CTGGAGGCCCAGGAGGACGTGACCCTGGAGCACCTGCCCGAGAGCGTGCTCCAGGATGTCATTCGCATC TCCCGCTGGCTGGTGGAATATGGCCGCAACCAAGATTTCATGAACGTCTACTACCAGATACGCTCCAGC CAGCTGGACCGCTCCATCAAAGGACTGAAGGAGCATTTCCATAAGAGCAGTTCTTCCTCTGGGGTTCCC TACTCCCCTGCTATCCCCAACAAGAGGAAAGACACACCTACCAAGAAGCCAGTCAAGCGGCCAGGGACG ATCCGTAAGGCTCAGAACCTTCTGAAACAGTATTCCCAGCATGGTCTAGATGGGAAAAAGGGGGGCTCT AACCTCATTCCTCTGGAAGGGAGAGATGACATGCTGGACGTGGAGACCGATGCCTACATCCACTGCGTC AGTGCCTTCGTCAAGCTGGCGCAGAGCGAGTACCAGCTGCTGGCCGACATCATCCCCGAGCACCACCAG AAGAAGACCTTCGACTCCCTGATACAGGATGCCCTGGATGGGCTGATGCTTGAAGGGGAGAACATCGTG TCTGCTGCCCGGAAGGCCATTGTGCGACACGACTTCTCCACGGTGCTCACCGTCTTCCCCATCCTGCGA CACCTCAAGCAGACCAAGCCTGAGTTTGACCAGGTGCTCCAGGGCACGGCTGCCAGCACAAAGAACAAG CTGCCTGGCCTCATCACATCCATGGAGACCATCGGTGCCAAAGCGCTGGAGGACTTCGCAGACAACATC AAGAATGACCCGGACAAGGAGTACAACATGCCGAAGGACGGCACCGTACACGAGCTCACCAGCAATGCC ATCCTCTTCCTGCAGCAGCTTTTGGACTTCCAGGAGACGGCAGGCGCCATGCTGGCCTCCCAAGTTCTT GGGGACACATACAATATTCCTTTAGACCCCCGAGAGACCAGCTCTTCGGCCACCAGCTACAGCTCTGAG TTCAGCAAGCGGCTGCTAAGCACCTATATCTGTAAAGTGCTGGGCAACCTGCAGTTGAACTTGCTGAGC AAGTCCAAGGTGTACGAGGACCCAGCTCTGAGCGCCATCTTCCTGCACAACAACTACAATTACATCCTC AAGTCCCTGGAGAAGTCTGAACTGATCCAGCTGGTGGCAGTGACACAGAAGACTGCTGAGCGCTCCTAC CGGGAGCACATTGAGCAGCAGATCCAGACCTACCAGCGCAGCTGGTTAAAGGTGACTGATTACATCGCA GAGAAGAATCTACCTGTGTTCCAGCCGGGAGTCAAGCTCCGGGACAAGGAGCGGCAGATTATCAAGGAG CGTTTTAAGGGCTTCAATGATGGCCTCGAAGAACTGTGCAAAATCCAGAAGGCCTGGGCTATTCCAGAC ACAGAGCAGAGGGACAGGATTCGCCAGGCCCAGAAGACCATTGTCAAGGAGACCTACGGGGCCTTTCTA CAGAAGTTTGGCAGCGTGCCCTTCACCAAGAACCCGGAGAAGTACATCAAGTACGGGGTGGAGCAGGTG GGCGACATGATCGATCGCCTTTTCGACACCTCTGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282313
Insert Size	1971 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282313.1
RefSeq Size	4834 bp
RefSeq ORF	1971 bp
Locus ID	23265
UniProt ID	Q9UPT5
Protein Families	Druggable Genome
Protein Pathways	Insulin signaling pathway
MW	74.7 kDa
Gene Summary	The protein encoded by this gene is a component of the exocyst complex. The exocyst complex plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. The encoded protein is required for assembly of the exocyst complex and docking of the complex to the plasma membrane. The encoded protein may also play a role in pre-mRNA splicing through interactions with pre-mRNA-processing factor 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG, and the resulting isoform (3) has a shorter N-terminus and is shorter compared to isoform 1.

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