ADAM2 (NM_001278114) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CRYN1; CRYN2; CT15; FTNB; PH-30b; PH30; PH30-beta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337175 representing NM_001278114. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGCGCGTCTTGTTTCTGCTCAGCGGGCTCGGCGGGCTGCGGATGGACAGTAATTTTGATAGTTTA CCTGTGCAAATTACAGTTCCGGAGAAAATACGGTCAATAATAAAGGAAGGAATTGAATCGCAGGCATCC TACAAAATTGTAATTGAAGGGAAACCATATACTGTGAATTTAATGCAAAAAAACTTTTTACCCCATAAT TTTAGAGTTTACAGTTATAGTGGCACAGGAATTATGAAACCACTTGACCAAGATTTTCAGAATTTCTGC CACTACCAAGGGTATATTGAAGGTTATCCAAAATCTGTGGTGATGGTTAGCACATGTACTGGACTCAGG GGCGTACTACAGTTTGAAAATGTTAGTTATGGAATAGAACCCCTGGAGTCTTCAGTTGGCTTTGAACAT GTAATTTACCAAGTAAAACATAAGAAAGCAGATGTTTCCTTATATAATGAGAAGGATATTGAATCAAGA GATCTGTCCTTTAAATTACAAAGCGTAGAGCCACAGCAAGATTTTGCAAAGTATATAGAAATGCATGTT ATAGTTGAAAAACAATTGTATAATCATATGGGGTCTGATACAACTGTTGTCGCTCAAAAAGTTTTCCAG TTGATTGGATTGACGAATGCTATTTTTGTTTCATTTAATATTACAATTATTCTGTCTTCATTGGAGCTT TGGATAGATGAAAATAAAATTGCAACCACTGGAGAAGCTAATGAGTTATTACACACATTTTTAAGATGG AAAACATCTTATCTTGTTTTACGTCCTCATGATGTGGCATTTTTACTTGTTTACAGAGAAAAGTCAAAT TATGTTGGTGCAACCTTTCAAGGGAAGATGTGTGATGCAAACTATGCAGGAGGTGTTGTTCTGCACCCC AGAACCATAAGTCTGGAATCACTTGCAGTTATTTTAGCTCAATTATTGAGCCTTAGTATGGGGATCACT TATGATGACATTAACAAATGCCAGTGCTCAGGAGCTGTCTGCATTATGAATCCAGAAGCAATTCATTTC AGTGGTGTGAAGATCTTTAGTAACTGCAGCTTCGAAGACTTTGCACATTTTATTTCAAAGCAGAAGTCC CAGTGTCTTCACAATCAGCCTCGCTTAGATCCTTTTTTCAAACAGCAAGCAGTGTGTGGTAATGCAAAG CTGGAAGCAGGAGAGGAGTGTGACTGTGGGACTGAACAGGATTGTGCCCTTATTGGAGAAACATGCTGT GATATTGCCACATGTAGATTTAAAGCCGGTTCAAACTGTGCTGAAGGACCATGCTGCGAAAACTGTCTA TTTATGTCAAAAGAAAGAATGTGTAGGCCTTCCTTTGAAGAATGCGACCTCCCTGAATATTGCAATGGA TCATCTGCATCATGCCCAGAAAACCACTATGTTCAGACTGGGCATCCGTGTGGACTGAATCAATGGATC TGTATAGATGGAGTTTGTATGAGTGGGGATAAACAATGTACAGACACATTTGGCAAAGAAGTAGAGTTT GGCCCTTCAGAATGTTATTCTCACCTTAATTCAAAGACTGATGTATCTGGAAACTGTGGTATAAGTGAT TCAGGATACACACAGTGTGAAGTTTGCAGGAATCAAAGATGTGTGAGTTCTTCATACTTGGGTTATGAT TGTACTACTGACAAATGCAATGATAGAGGTGTATGCAATAACAAAAAGCACTGTCACTGTAGTGCTTCA TATTTACCTCCAGATTGCTCAGTTCAATCAGATCTATGGCCTGGTGGGAGTATTGACAGTGGCAATTTT CCACCTGTAGCTATACCAGCCAGACTCCCTGAAAGGCGCTACATTGAGAACATTTACCATTCCAAACCA ATGAGATGGCCATTTTTCTTATTCATTCCTTTCTTTATTATTTTCTGTGTACTGATTGCTATAATGGTG AAAGTTAATTTCCAAAGGAAAAAATGGAGAACTGAGGACTATTCAAGCGATGAGCAACCTGAAAGTGAG AGTGAACCTAAAGGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278114
Insert Size	2019 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278114.1
RefSeq Size	2495 bp
RefSeq ORF	2019 bp
Locus ID	2515
UniProt ID	Q99965
Protein Families	Druggable Genome, Protease, Transmembrane
MW	75.6 kDa
Gene Summary	This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded protein is a subunit of an integral sperm membrane glycoprotein called fertilin, which plays an important role in sperm-egg interactions. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013] Transcript Variant: This variant (3) uses an alternate splice site and lacks an internal exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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