LARGE2 (NM_001300722) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GYLTL1B; PP5656
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300722, the custom clone sequence may differ by one or more nucleotides ATGCTGCCCCGAGGGCGCCCCCGGGCGCTGGGGGCCGCCGCGCTGTTGCTGCTGCTGCTGCTGCTCGGAT TCCTCCTGTTCGACGGGAGGCTGCGGAGAGCCGCCGCCCTCGACGGAGACCCGGGGGCCGGCCCCGGGGA CCACAACCGCTCCGACTGCGGCCCGCAGCCGCCGCCGCCGCCCAAGTGCGAGCTCTTGCATGTGGCCATC GTGTGTGCGGGGCATAACTCCAGCCGAGACGTCATCACCCTGGTGAAGTCCATGCTCTTCTACAGGAAAA ATCCACTGCACCTCCACTTGGTGACTGACGCCGTGGCCAGAAACATCCTGGAGACGCTCTTCCACACATG GATGGTGCCTGCTGTCCGTGTCAGCTTTTATCATGCCGACCAGCTCAAGCCCCAGGTCTCCTGGATCCCC AACAAGCACTACTCCGGCCTCTATGGGCTAATGAAGCTGGTGCTGCCCAGTGCCTTGCCTGCTGAGCTGG CCCGCGTCATTGTCCTGGACACGGATGTCACCTTCGCCTCTGACATCTCGGAGCTCTGGGCCCTCTTTGC TCACTTTTCTGACACGCAGGCGATCGGTCTTGTGGAGAACCAGAGTGACTGGTACCTGGGCAACCTCTGG AAGAACCACAGGCCCTGGCCTGCCTTGGGCCGGGGATTTAACACAGGTGTGATCCTGCTGCGGCTGGACC GGCTCCGGCAGGCTGGCTGGGAGCAGATGTGGAGGCTGACAGCCAGGCGGGAGCTCCTTAGCCTGCCTGC CACCTCACTGGCTGACCAGGACATCTTCAACGCTGTGATCAAGGAGCACCCGGGGCTAGTGCAGCGTCTG CCTTGTGTCTGGAATGTGCAGCTGTCAGATCACACACTGGCCGAGCGCTGCTACTCTGAGGCGTCTGACC TCAAGGTGATCCACTGGAACTCACCAAAGAAGCTTCGGGTGAAGAACAAGCATGTGGAATTCTTCCGCAA TTTCTACCTGACCTTCCTGGAGTACGATGGGAACCTGCTGCGGAGAGAGCTCTTTGTGTGCCCCAGCCAG CCCCCACCTGGTGCTGAGCAGTTGCAGCAGGCCCTGGCACAACTGGACGAGGAAGACCCCTGCTTTGAGT TCCGGCAGCAGCAGCTCACTGTGCACCGTGTGCATGTCACTTTCCTGCCCCATGAACCGCCACCCCCCCG GCCTCACGATGTCACCCTTGTGGCCCAGCTGTCCATGGACCGGCTGCAGATGTTGGAAGCCCTGTGCAGG CACTGGCCTGGCCCCATGAGCCTGGCCTTGTACCTGACAGACGCAGAAGCTCAGCAGTTCCTGCATTTCG TCGAGGCCTCACCAGTGCTTGCTGCCCGGCAGGACGTGGCCTACCATGTGGTGTACCGTGAGGGGCCCCT ATACCCCGTCAACCAGCTTCGCAACGTGGCCTTGGCCCAGGCCCTCACGCCTTACGTCTTCCTCAGTGAC ATTGACTTCCTGCCTGCCTATTCTCTCTACGACTACCTCAGGGCCTCCATTGAGCAGCTGGGGCTGGGCA GCCGGCGCAAGGCAGCACTGGTGGTGCCGGCATTCGAGACCCTGCGCTACCGCTTCAGCTTCCCCCATTC CAAGGTGGAGCTGTTGGCCTTGCTGGATGCGGGCACTCTCTACACCTTCAGGTACCACGAGTGGCCCCGA GGCCACGCACCCACAGACTATGCCCGCTGGCGGGAGGCTCAGGCCCCGTACCGTGTGCAATGGGCGGCCA ACTATGAACCCTACGTGGTGGTGCCACGAGACTGTCCCCGCTATGATCCTCGCTTTGTGGGCTTCGGCTG GAACAAAGTGGCCCACATTGTGGAGCTGGATGCCCAGGAATATGAGCTCCTGGTGCTGCCCGAGGCCTTC ACCATCCATCTGCCCCACGCTCCAAGCCTGGACATCTCCCGCTTCCGCTCCAGCCCCACCTATCGTGACT GCCTCCAGGCCCTCAAGGACGAATTCCACCAGGACTTGTCCCGCCACCATGGGGCTGCTGCCCTCAAATA CCTCCCAGCCCTGCAGCAGCCCCAGAGCCCTGCCCGAGGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300722
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300722.1, NP_001287651.1
RefSeq Size	2473 bp
RefSeq ORF	2073 bp
Locus ID	120071
UniProt ID	Q8N3Y3
Protein Families	Transmembrane
Gene Summary	Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an in-frame segment compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.