LARGE2 (NM_001300722) Human Tagged ORF Clone
CAT#: RC239315
- TrueORF®
GYLTL1B (myc-DDK-tagged) - Human glycosyltransferase-like 1B (GYLTL1B), transcript variant 3
ORF Plasmid: tGFP
"NM_001300722" in other vectors (2)
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CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GYLTL1B; PP5656 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239315 representing NM_001300722
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCCGAGGGCGCCCCCGGGCGCTGGGGGCCGCCGCGCTGTTGCTGCTGCTGCTGCTGCTCGGAT TCCTCCTGTTCGACGGGAGGCTGCGGAGAGCCGCCGCCCTCGACGGAGACCCGGGGGCCGGCCCCGGGGA CCACAACCGCTCCGACTGCGGCCCGCAGCCGCCGCCGCCGCCCAAGTGCGAGCTCTTGCATGTGGCCATC GTGTGTGCGGGGCATAACTCCAGCCGAGACGTCATCACCCTGGTGAAGTCCATGCTCTTCTACAGGAAAA ATCCACTGCACCTCCACTTGGTGACTGACGCCGTGGCCAGAAACATCCTGGAGACGCTCTTCCACACATG GATGGTGCCTGCTGTCCGTGTCAGCTTTTATCATGCCGACCAGCTCAAGCCCCAGGTCTCCTGGATCCCC AACAAGCACTACTCCGGCCTCTATGGGCTAATGAAGCTGGTGCTGCCCAGTGCCTTGCCTGCTGAGCTGG CCCGCGTCATTGTCCTGGACACGGATGTCACCTTCGCCTCTGACATCTCGGAGCTCTGGGCCCTCTTTGC TCACTTTTCTGACACGCAGGCGATCGGTCTTGTGGAGAACCAGAGTGACTGGTACCTGGGCAACCTCTGG AAGAACCACAGGCCCTGGCCTGCCTTGGGCCGGGGATTTAACACAGGTGTGATCCTGCTGCGGCTGGACC GGCTCCGGCAGGCTGGCTGGGAGCAGATGTGGAGGCTGACAGCCAGGCGGGAGCTCCTTAGCCTGCCTGC CACCTCACTGGCTGACCAGGACATCTTCAACGCTGTGATCAAGGAGCACCCGGGGCTAGTGCAGCGTCTG CCTTGTGTCTGGAATGTGCAGCTGTCAGATCACACACTGGCCGAGCGCTGCTACTCTGAGGCGTCTGACC TCAAGGTGATCCACTGGAACTCACCAAAGAAGCTTCGGGTGAAGAACAAGCATGTGGAATTCTTCCGCAA TTTCTACCTGACCTTCCTGGAGTACGATGGGAACCTGCTGCGGAGAGAGCTCTTTGTGTGCCCCAGCCAG CCCCCACCTGGTGCTGAGCAGTTGCAGCAGGCCCTGGCACAACTGGACGAGGAAGACCCCTGCTTTGAGT TCCGGCAGCAGCAGCTCACTGTGCACCGTGTGCATGTCACTTTCCTGCCCCATGAACCGCCACCCCCCCG GCCTCACGATGTCACCCTTGTGGCCCAGCTGTCCATGGACCGGCTGCAGATGTTGGAAGCCCTGTGCAGG CACTGGCCTGGCCCCATGAGCCTGGCCTTGTACCTGACAGACGCAGAAGCTCAGCAGTTCCTGCATTTCG TCGAGGCCTCACCAGTGCTTGCTGCCCGGCAGGACGTGGCCTACCATGTGGTGTACCGTGAGGGGCCCCT ATACCCCGTCAACCAGCTTCGCAACGTGGCCTTGGCCCAGGCCCTCACGCCTTACGTCTTCCTCAGTGAC ATTGACTTCCTGCCTGCCTATTCTCTCTACGACTACCTCAGGGCCTCCATTGAGCAGCTGGGGCTGGGCA GCCGGCGCAAGGCAGCACTGGTGGTGCCGGCATTCGAGACCCTGCGCTACCGCTTCAGCTTCCCCCATTC CAAGGTGGAGCTGTTGGCCTTGCTGGATGCGGGCACTCTCTACACCTTCAGGTACCACGAGTGGCCCCGA GGCCACGCACCCACAGACTATGCCCGCTGGCGGGAGGCTCAGGCCCCGTACCGTGTGCAATGGGCGGCCA ACTATGAACCCTACGTGGTGGTGCCACGAGACTGTCCCCGCTATGATCCTCGCTTTGTGGGCTTCGGCTG GAACAAAGTGGCCCACATTGTGGAGCTGGATGCCCAGGAATATGAGCTCCTGGTGCTGCCCGAGGCCTTC ACCATCCATCTGCCCCACGCTCCAAGCCTGGACATCTCCCGCTTCCGCTCCAGCCCCACCTATCGTGACT GCCTCCAGGCCCTCAAGGACGAATTCCACCAGGACTTGTCCCGCCACCATGGGGCTGCTGCCCTCAAATA CCTCCCAGCCCTGCAGCAGCCCCAGAGCCCTGCCCGAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239315 representing NM_001300722
Red=Cloning site Green=Tags(s) MLPRGRPRALGAAALLLLLLLLGFLLFDGRLRRAAALDGDPGAGPGDHNRSDCGPQPPPPPKCELLHVAI VCAGHNSSRDVITLVKSMLFYRKNPLHLHLVTDAVARNILETLFHTWMVPAVRVSFYHADQLKPQVSWIP NKHYSGLYGLMKLVLPSALPAELARVIVLDTDVTFASDISELWALFAHFSDTQAIGLVENQSDWYLGNLW KNHRPWPALGRGFNTGVILLRLDRLRQAGWEQMWRLTARRELLSLPATSLADQDIFNAVIKEHPGLVQRL PCVWNVQLSDHTLAERCYSEASDLKVIHWNSPKKLRVKNKHVEFFRNFYLTFLEYDGNLLRRELFVCPSQ PPPGAEQLQQALAQLDEEDPCFEFRQQQLTVHRVHVTFLPHEPPPPRPHDVTLVAQLSMDRLQMLEALCR HWPGPMSLALYLTDAEAQQFLHFVEASPVLAARQDVAYHVVYREGPLYPVNQLRNVALAQALTPYVFLSD IDFLPAYSLYDYLRASIEQLGLGSRRKAALVVPAFETLRYRFSFPHSKVELLALLDAGTLYTFRYHEWPR GHAPTDYARWREAQAPYRVQWAANYEPYVVVPRDCPRYDPRFVGFGWNKVAHIVELDAQEYELLVLPEAF TIHLPHAPSLDISRFRSSPTYRDCLQALKDEFHQDLSRHHGAAALKYLPALQQPQSPARG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001300722 |
ORF Size | 2070 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001300722.2 |
RefSeq Size | 2473 bp |
RefSeq ORF | 2073 bp |
Locus ID | 120071 |
UniProt ID | Q8N3Y3 |
Protein Families | Transmembrane |
MW | 79.2 kDa |
Gene Summary | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |