MED15 (NM_001293237) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1; TNRC7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001293237, the custom clone sequence may differ by one or more nucleotides ATGAGGAAAGCTGGTGTGGCACACAGTAAATCCAGCAAGGATATGGAGAGCCATGTTTTCCTGAAGGCCA AGACCCGGGACGAATACCTTTCTCTCGTGGCCAGGCTCATTATCCATTTTCGAGACATTCATAACAAGAA ATCTCAAGCTTCCGTCAGTGATCCTATGAATGCACTCCAGAGCCTGACTGGCGGACCTGCTGCGGGAGCC GCTGGAATTGGCATGCCTCCTCGGGGCCCGGGACAGTCTCTGGGCGGGATGGGTAGCCTTGGTGCCATGG GACAGCCAATGTCTCTCTCAGGGCAGCCGCCTCCTGGGACCTCGGGGATGGCCCCTCACAGCATGGCTGT CGTGTCTACGGCAACTCCACAGACCCAGCTGCAGCTCCAGCAGGTGGCGCTGCAGCAGCAGCAGCAACAG CAGCAGTTCCAGCAGCAGCAGCAGGCGGCGCTACAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGTTCC AGGCTCAGCAGAGTGCCATGCAGCAGCAGTTCCAAGCAGTAGTGCAGCAGCAGCAGCAGCTCCAGCAGCA GCAGCAGCAGCAGCAGCATCTAATTAAATTGCATCATCAAAATCAGCAACAGATACAGCAGCAGCAACAG CAGCTGCAGCGAATAGCACAGCTGCAGCTCCAACAACAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGC AGCAGCAGGCTTTGCAGGCCCAGCCACCAATTCAGCAGCCACCGATGCAGCAGCCACAGCCTCCGCCCTC CCAGGCTCTGCCCCAGCAGCTGCAGCAGATGCATCACACACAGCACCACCAGCCGCCACCACAGCCCCAG CAGCCTCCAGTTGCTCAGAACCAACCATCACAACTCCCGCCACAGTCGCAGACCCAGCCTTTGGTGTCAC AGGCGCAAGCTCTCCCTGGACAAATGTTGTATACCCAACCACCACTGAAATTTGTCCGAGCTCCGATGGT GGTGCAGCAGCCCCCAGTGCAGCCCCAGGTGCAGCAGCAGCAGACAGCAGTACAGACAGCTCAGGCTGCC CAGATGGTGGCTCCCGGAGTCCAGGTCAGCCAGAGCAGCCTCCCCATGCTGTCCTCGCCGTCACCGGGCC AGCAGGTGCAGACCCCGCAGTCGATGCCCCCTCCCCCCCAGCCGTCCCCGCAGCCCGGCCAGCCCAGCTC ACAGCCCAACTCCAACGTCAGCTCTGGCCCTGCCCCATCTCCCAGTAGCTTCCTGCCCAGCCCCTCACCG CAGCCCTCCCAGAGCCCAGTGACGGCGCGGACCCCACAGAACTTCAGTGTCCCCTCACCTGGACCTTTAA ACACACCTGTGAACCCCAGCTCTGTCATGAGCCCAGCTGGCTCCAGCCAGGCTGAGGAGCAGCAGTACCT GGACAAGCTGAAGCAGCTGTCGAAGTACATCGAGCCCCTGCGCCGCATGATCAACAAGATCGACAAGAAC GAAGACAGAAAAAAGGACCTGAGTAAGATGAAGAGCCTTCTGGACATTCTGACAGACCCCTCGAAGCGGT GTCCCCTGAAGACCTTGCAAAAGTGTGAGATCGCCCTGGAGAAACTCAAGAATGACATGGCGGTGCCCAC TCCCCCACCGCCCCCGGTGCCACCGACCAAACAGCAGTACCTATGCCAGCCGCTCCTGGATGCCGTCCTG GCCAACATCCGCTCACCTGTCTTCAACCATTCCCTGTACCGCACATTCGTTCCAGCCATGACCGCCATTC ACGGCCCACCCATCACGGCCCCAGTGGTGTGCACCCGGAAGCGCAGGCTTGAGGATGATGAGCGGCAGAG CATCCCCAGTGTGCTCCAGGGTGAGGTGGCCAGGCTGGACCCCAAGTTCCTGGTAAACCTGGACCCTTCT CACTGCAGCAACAATGGCACTGTCCACCTGATCTGCAAGCTGGATGACAAGGACCTCCCAAGTGTGCCAC CACTGGAGCTCAGTGTGCCCGCTGACTATCCTGCCCAAAGCCCGCTGTGGATAGACCGGCAGTGGCAGTA CGACGCCAACCCCTTCCTCCAGTCGGTGCACCGCTGCATGACCTCCAGGCTGCTGCAGCTCCCGGACAAG CACTCGGTCACCGCCTTGCTCAACACCTGGGCCCAGAGCGTCCACCAGGCCTGCCTCTCAGCCGCCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001293237
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293237.1, NP_001280166.1
RefSeq Size	3523 bp
RefSeq ORF	2169 bp
Locus ID	51586
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' and 3' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded protein (isoform d) is shorter, compared to isoform a. Variants 4 and 6 encode the same protein (isoform d).

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