BRRN1 (NCAPH) (NM_001281710) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRRN1; CAP-H; CAPH; MCPH23
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001281710, the custom clone sequence may differ by one or more nucleotides ATGGGACCTCCCGGCCCAGAGACGCGAGGACACCCCCACAGTGCCTCCTCTCCTTCAGAGCGTGTGTTCC CGATGCCCCTGCCCAGGAAGGCGCCTCTCAATATTCCTGGCACCCCAGTCCTCGAAGACTTTCCTCAGAA TGACGATGAGAAGGAGCGGCTGCAGCGGAGGCGCTCGAGGGTCTTTGATCTGCAGTTCAGCACTGACTCA CCTCGCTTATTGGCCTCCCCCTCCAGCAGGAGTATTGACATTTCAGCTACTATCCCCAAGTTTACAAACA CGCAGATTACGGAACATTACTCCACCTGTATCAAACTGTCCACTGAAAATAAAATCACTACCAAGAATGC TTTTGGTTTGCACTTGATTGATTTTATGTCAGAGATTCTTAAACAGAAAGACACCGAACCAACCAACTTT AAAGTGGCTGCGGGTACTCTGGATGCCAGCACCAAGATCTATGCTGTGCGCGTGGATGCCGTCCATGCCG ATGTATACAGAGTCCTTGGGGGGCTGGGCAAAGATGCACCGTCTTTGGAAGAAGTAGAAGGCCATGTTGC TGATGGAAGTGCTACTGAAATGGGAACAACCAAAAAGGCTGTAAAGCCAAAGAAGAAGCACTTACACAGA ACTATTGAGCAGAACATAAACAACCTCAATGTCTCCGAAGCAGATCGGAAGTGTGAGATTGATCCCATGT TTCAGAAGACAGCAGCCTCATTTGATGAGTGCAGCACAGCAGGGGTGTTTCTGTCCACTCTCCACTGCCA GGACTACAGAAGTGAACTGCTGTTTCCCTCTGATGTCCAGACTCTCTCCACGGGAGAACCTCTCGAGTTG CCAGAGTTAGGTTGTGTAGAAATGACAGATTTAAAAGCGCCCTTGCAGCAGTGTGCAGAAGATCGCCAGA TCTGCCCTTCCCTGGCCGGGTTCCAGTTTACACAGTGGGACAGTGAAACACATAATGAGTCTGTGTCGGC CCTGGTAGACAAGTTTAAGAAGAATGACCAGGTATTTGACATCAATGCTGAAGTTGACGAGAGTGACTGT GGAGACTTCCCCGATGGGTCCCTGGGGGATGACTTTGATGCCAACGATGAACCTGACCACACCGCAGTTG GGGATCATGAAGAGTTCAGGAGCTGGAAGGAGCCCTGCCAGGTTCAGAGCTGCCAGGAAGAAATGATTTC CCTTGGGGATGGAGACATCAGGACCATGTGCCCCCTTCTGTCTATGAAACCTGGAGAATATTCTTATTTC AGTCCTCGGACCATGTCGATGTGGGCTGGCCCGGATCACTGGCGCTTTAGGCCTCGACGCAAACAAGATG CTCCTTCCCAATCAGAAAACAAAAAGAAGAGTACAAAAAAAGATTTTGAAATTGACTTTGAAGATGATAT TGACTTTGATGTATATTTTAGAAAAACAAAGGCTGCTACTATTCTGACCAAGTCCACTTTGGAGAACCAG AATTGGAGAGCTACCACCCTTCCTACAGATTTCAACTACAATGTTGACACTCTGGTCCAGCTTCACCTCA AACCAGGCACCAGGTTACTTAAGATGGCCCAGGGCCATAGGGTAGAGACTGAGCATTATGAAGAAATTGA AGACTATGATTACAACAACCCTAACGACACCTCCAACTTTTGCCCTGGATTACAGGCTGCTGACAGTGAT GATGAAGATTTGGATGACTTATTTGTGGGACCTGTTGGGAACTCTGACCTCTCACCTTATCCTTGCCATC CACCTAAGACAGCACAACAGAATGGTGACACTCCAGAAGCCCAAGGATTAGACATCACAACATATGGGGA GTCAAACTTGGTAGCTGAGCCTCAGAAGGTAAATAAAATTGAAATTCACTATGCCAAGACTGCCAAAAAG ATGGACATGAAGAAACTGAAGCAGAGCATGTGGAGTCTGCTGACAGCGCTCTCCGGAAAGGAGGCAGATG CAGAGGCAAACCACAGGGAAGCTGGAAAAGAAGCGGCCCTGGCAGAAGTGGCTGACGAGAAGATGCTTAG CGGGCTCACGAAGGACCTGCAGAGGAGCCTGCCCCCTGTCATGGCTCAGAACCTCTCCATACCTCTGGCT TTTGCCTGTCTCCTACATTTAGCCAATGAAAAGAATCTAAAACTGGAAGGAACAGAGGACCTCTCTGATG TTCTTGTGAGGCAAGGAGATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001281710
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281710.1, NP_001268639.1
RefSeq Size	6026 bp
RefSeq ORF	2193 bp
Locus ID	23397
UniProt ID	Q15003
Gene Summary	This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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