ECEL1 (NM_001290787) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DA5D; DINE; ECEX; XCE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290787, the custom clone sequence may differ by one or more nucleotides ATGGAGCCCCCGTATTCGCTGACGGCGCACTACGATGAGTTCCAAGAGGTCAAGTACGTGAGCCGCTGCG GCGCGGGGGGCGCGCGCGGGGCCTCCCTGCCCCCGGGCTTCCCGTTGGGCGCTGCGCGCAGCGCCACCGG GGCCCGGTCCGGGCTGCCGCGCTGGAACCGGCGCGAGGTGTGCCTGCTGTCGGGGCTGGTGTTCGCCGCC GGCCTCTGCGCCATTCTGGCGGCTATGCTGGCCCTCAAGTACCTGGGCCCGGTCGCGGCCGGCGGCGGCG CCTGTCCCGAGGGCTGCCCTGAGCGCAAGGCCTTCGCGCGCGCCGCTCGCTTCCTGGCCGCCAACCTGGA CGCCAGCATCGACCCATGCCAGGACTTCTACTCGTTCGCCTGCGGCGGTTGGCTGCGGCGCCACGCCATC CCCGACGACAAGCTCACCTATGGCACCATCGCGGCCATCGGCGAGCAAAACGAGGAGCGCCTACGGCGCC TGCTGGCGCGGCCCGGGGGTGGGCCTGGCGGCGCGGCCCAGCGCAAGGTGCGCGCCTTCTTCCGCTCGTG CCTCGACATGCGCGAGATCGAGCGACTGGGCCCGCGACCCATGCTAGAGGTCATCGAGGACTGCGGGGGC TGGGACCTGGGCGGCGCGGAGGAGCGTCCGGGGGTCGCGGCGCGATGGGACCTCAACCGGCTGCTGTACA AGGCGCAGGGCGTGTACAGCGCCGCCGCGCTCTTCTCGCTCACGGTCAGCCTGGACGACAGGAACTCCTC GCGCTACGTCATCCGCATTGACCAGGATGGGCTCACCCTGCCAGAGAGGACCCTGTACCTCGCTCAGGAT GAGGACAGTGAGAAGATCCTGGCAGCATACAGGGTGTTCATGGAGCGAGTGCTCAGCCTCCTGGGTGCAG ACGCTGTGGAACAGAAGGCCCAAGAGATCCTGCAAGTGGAGCAGCAGCTGGCCAACATCACTGTGTCAGA GCATGACGACCTACGGCGAGATGTCAGCTCCATGTACAACAAGGTGACGCTGGGGCAGCTGCAGAAGATC ACCCCCCACTTGCGGTGGAAGTGGCTGCTAGACCAGATCTTCCAGGAGGACTTCTCAGAGGAAGAGGAGG TGGTGCTGCTGGCGACAGACTACATGCAGCAGGTGTCGCAGCTCATCCGCTCCACACCCCACCGGGTCCT GCACAACTACCTGGTGTGGCGCGTGGTGGTGGTCCTGAGTGAACACCTGTCCCCGCCATTCCGTGAGGCA CTGCACGAGCTGGCACAGGAGATGGAGGGCAGCGACAAGCCACAGGAGCTGGCCCGGGTCTGCTTGGGCC AGGCCAATCGCCACTTTGGCATGGCGCTTGGCGCCCTCTTTGTACATGAGCACTTCTCAGCTGCCAGCAA AGCCAAGGTGCAGCAGCTAGTGGAAGACATCAAGTACATCCTGGGCCAGCGCCTGGAGGAGCTGGACTGG ATGGACGCCGAGACCAGGGCTGCTGCTCGGGCCAAGCTCCAGTACATGATGGTGATGGTCGGCTACCCGG ACTTCCTGCTGAAACCCGATGCTGTGGACAAGGAGTATGAGTTTGAGGTCCATGAGAAGACCTACTTCAA GAACATCTTGAACAGCATCCGCTTCAGCATCCAGCTCTCAGTTAAGAAGATTCGGCAGGAGGTGGACAAG TGGCTGCTCCCCCCACAGGCGCTCAATGCCTACTATCTACCCAACAAGAACCAGATGGTGTTCCCCGCGG GCATCCTGCAGCCCACCCTGTACGACCCTGACTTCCCACAGTCTCTCAACTACGGGGGCATCGGCACCAT CATTGGACATGAGCTGACCCACGGCTACGACGACTGGGGGGGCCAGTATGACCGCTCAGGGAACCTGCTG CACTGGTGGACGGAGGCCTCCTACAGCCGCTTCCTGCGAAAGGCTGAGTGCATCGTCCGTCTCTATGACA ACTTCACTGTCTACAACCAGCGGGTGAACGGGAAACACACGCTTGGGGAGAACATCGCAGATATGGGCGG CCTCAAGCTGGCCTACCACGCCTATCAGAAGTGGGTGCGGGAGCACGGCCCAGAGCACCCACTTCCCCGG CTCAAGTACACACATGACCAGCTCTTCTTCATTGCCTTTGCCCAGAACTGGTGCATCAAGCGGCGGTCGC AGTCCATCTACCTGCAGGTGCTGACTGACAAGCATGCCCCTGAGCACTACAGGGTGCTGGGCAGTGTGTC CCAGTTTGAGGAGTTTGGCCGGGCTTTCCACTGTCCCAAGGACTCACCCATGAACCCTGCCCACAAGTGT TCCGTGTGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001290787
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290787.1, NP_001277716.1
RefSeq Size	2903 bp
RefSeq ORF	2322 bp
Locus ID	9427
UniProt ID	O95672
Protein Families	Druggable Genome, Protease, Transmembrane
Gene Summary	This gene encodes a member of the M13 family of endopeptidases. Members of this family are zinc-containing type II integral-membrane proteins that are important regulators of neuropeptide and peptide hormone activity. Mutations in this gene are associated with autosomal recessive distal arthrogryposis, type 5D. This gene has multiple pseudogenes on chromosome 2. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.