ECEL1 (NM_001290787) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DA5D; DINE; ECEX; XCE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239537 representing NM_001290787 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCCGTATTCGCTGACGGCGCACTACGATGAGTTCCAAGAGGTCAAGTACGTGAGCCGCTGCG GCGCGGGGGGCGCGCGCGGGGCCTCCCTGCCCCCGGGCTTCCCGTTGGGCGCTGCGCGCAGCGCCACCGG GGCCCGGTCCGGGCTGCCGCGCTGGAACCGGCGCGAGGTGTGCCTGCTGTCGGGGCTGGTGTTCGCCGCC GGCCTCTGCGCCATTCTGGCGGCTATGCTGGCCCTCAAGTACCTGGGCCCGGTCGCGGCCGGCGGCGGCG CCTGTCCCGAGGGCTGCCCTGAGCGCAAGGCCTTCGCGCGCGCCGCTCGCTTCCTGGCCGCCAACCTGGA CGCCAGCATCGACCCATGCCAGGACTTCTACTCGTTCGCCTGCGGCGGTTGGCTGCGGCGCCACGCCATC CCCGACGACAAGCTCACCTATGGCACCATCGCGGCCATCGGCGAGCAAAACGAGGAGCGCCTACGGCGCC TGCTGGCGCGGCCCGGGGGTGGGCCTGGCGGCGCGGCCCAGCGCAAGGTGCGCGCCTTCTTCCGCTCGTG CCTCGACATGCGCGAGATCGAGCGACTGGGCCCGCGACCCATGCTAGAGGTCATCGAGGACTGCGGGGGC TGGGACCTGGGCGGCGCGGAGGAGCGTCCGGGGGTCGCGGCGCGATGGGACCTCAACCGGCTGCTGTACA AGGCGCAGGGCGTGTACAGCGCCGCCGCGCTCTTCTCGCTCACGGTCAGCCTGGACGACAGGAACTCCTC GCGCTACGTCATCCGCATTGACCAGGATGGGCTCACCCTGCCAGAGAGGACCCTGTACCTCGCTCAGGAT GAGGACAGTGAGAAGATCCTGGCAGCATACAGGGTGTTCATGGAGCGAGTGCTCAGCCTCCTGGGTGCAG ACGCTGTGGAACAGAAGGCCCAAGAGATCCTGCAAGTGGAGCAGCAGCTGGCCAACATCACTGTGTCAGA GCATGACGACCTACGGCGAGATGTCAGCTCCATGTACAACAAGGTGACGCTGGGGCAGCTGCAGAAGATC ACCCCCCACTTGCGGTGGAAGTGGCTGCTAGACCAGATCTTCCAGGAGGACTTCTCAGAGGAAGAGGAGG TGGTGCTGCTGGCGACAGACTACATGCAGCAGGTGTCGCAGCTCATCCGCTCCACACCCCACCGGGTCCT GCACAACTACCTGGTGTGGCGCGTGGTGGTGGTCCTGAGTGAACACCTGTCCCCGCCATTCCGTGAGGCA CTGCACGAGCTGGCACAGGAGATGGAGGGCAGCGACAAGCCACAGGAGCTGGCCCGGGTCTGCTTGGGCC AGGCCAATCGCCACTTTGGCATGGCGCTTGGCGCCCTCTTTGTACATGAGCACTTCTCAGCTGCCAGCAA AGCCAAGGTGCAGCAGCTAGTGGAAGACATCAAGTACATCCTGGGCCAGCGCCTGGAGGAGCTGGACTGG ATGGACGCCGAGACCAGGGCTGCTGCTCGGGCCAAGCTCCAGTACATGATGGTGATGGTCGGCTACCCGG ACTTCCTGCTGAAACCCGATGCTGTGGACAAGGAGTATGAGTTTGAGGTCCATGAGAAGACCTACTTCAA GAACATCTTGAACAGCATCCGCTTCAGCATCCAGCTCTCAGTTAAGAAGATTCGGCAGGAGGTGGACAAG TGGCTGCTCCCCCCACAGGCGCTCAATGCCTACTATCTACCCAACAAGAACCAGATGGTGTTCCCCGCGG GCATCCTGCAGCCCACCCTGTACGACCCTGACTTCCCACAGTCTCTCAACTACGGGGGCATCGGCACCAT CATTGGACATGAGCTGACCCACGGCTACGACGACTGGGGGGGCCAGTATGACCGCTCAGGGAACCTGCTG CACTGGTGGACGGAGGCCTCCTACAGCCGCTTCCTGCGAAAGGCTGAGTGCATCGTCCGTCTCTATGACA ACTTCACTGTCTACAACCAGCGGGTGAACGGGAAACACACGCTTGGGGAGAACATCGCAGATATGGGCGG CCTCAAGCTGGCCTACCACGCCTATCAGAAGTGGGTGCGGGAGCACGGCCCAGAGCACCCACTTCCCCGG CTCAAGTACACACATGACCAGCTCTTCTTCATTGCCTTTGCCCAGAACTGGTGCATCAAGCGGCGGTCGC AGTCCATCTACCTGCAGGTGCTGACTGACAAGCATGCCCCTGAGCACTACAGGGTGCTGGGCAGTGTGTC CCAGTTTGAGGAGTTTGGCCGGGCTTTCCACTGTCCCAAGGACTCACCCATGAACCCTGCCCACAAGTGT TCCGTGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239537 representing NM_001290787 Red=Cloning site Green=Tags(s) MEPPYSLTAHYDEFQEVKYVSRCGAGGARGASLPPGFPLGAARSATGARSGLPRWNRREVCLLSGLVFAA GLCAILAAMLALKYLGPVAAGGGACPEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAI PDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGG WDLGGAEERPGVAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQD EDSEKILAAYRVFMERVLSLLGADAVEQKAQEILQVEQQLANITVSEHDDLRRDVSSMYNKVTLGQLQKI TPHLRWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPHRVLHNYLVWRVVVVLSEHLSPPFREA LHELAQEMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDW MDAETRAAARAKLQYMMVMVGYPDFLLKPDAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDK WLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLL HWWTEASYSRFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYHAYQKWVREHGPEHPLPR LKYTHDQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAHKC SVW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290787
ORF Size	2319 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290787.2
RefSeq Size	2903 bp
RefSeq ORF	2322 bp
Locus ID	9427
UniProt ID	O95672
Protein Families	Druggable Genome, Protease, Transmembrane
MW	88.1 kDa
Gene Summary	This gene encodes a member of the M13 family of endopeptidases. Members of this family are zinc-containing type II integral-membrane proteins that are important regulators of neuropeptide and peptide hormone activity. Mutations in this gene are associated with autosomal recessive distal arthrogryposis, type 5D. This gene has multiple pseudogenes on chromosome 2. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]

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