EPB41L3 (NM_001281533) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	4.1B; DAL-1; DAL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001281533, the custom clone sequence may differ by one or more nucleotides ATGACGACCGAATCTGGATCAGACTCGGAATCCAAGCCGGACCAGGAGGCCGAGCCCCAGGAGGCGGCGG GGGCGCAGGGGCGCGCGGGGGCGCCCGTGCCGGAGCCGCCCAAGGAGGAGCAGCAGCAGGCCCTGGAGCA GTTCGCCGCCGCTGCAGCGCACAGCACCCCGGTGCGGAGGGAGGTCACTGACAAGGAACAGGAGTTTGCT GCCAGGGCTGCAAAACAGCTCGAATATCAGCAATTAGAAGACGATAAACTTTCTCAGAAATCATCTAGCA GTAAACTCTCTCGGTCTCCATTAAAGATTGTCAAAAAGCCTAAAAGCATGCAGTGCAAAGTGATACTTCT CGATGGATCAGAATATACCTGTGATGTAGAGAAACGCTCCAGAGGACAAGTGCTGTTTGATAAAGTGTGT GAACACTTGAACTTGCTAGAGAAAGACTACTTTGGGCTTACGTATCGAGATGCTGAAAACCAGAAGAATT GGTTGGACCCTGCTAAGGAAATAAAAAAACAGGTTCGAAGTGGTGCTTGGCACTTTTCATTTAATGTGAA ATTTTATCCACCAGACCCTGCCCAACTATCTGAAGATATCACCAGGTACTACCTCTGCTTGCAGTTGCGA GATGACATCGTGTCCGGAAGGCTGCCCTGCTCCTTTGTTACCCTGGCCTTGCTGGGCTCCTACACTGTCC AGTCAGAGCTCGGAGACTATGACCCAGATGAATGTGGGAGCGATTACATTAGTGAGTTCCGCTTTGCACC AAACCACACTAAAGAACTGGAAGACAAAGTGATCGAGCTGCACAAGAGCCACAGAGGAATGACGCCAGCA GAAGCAGAGATGCATTTCTTGGAAAATGCCAAAAAATTATCAATGTATGGGGTAGATTTACATCATGCTA AGGACTCAGAAGGGGTAGAAATTATGTTAGGAGTTTGTGCAAGTGGTCTGTTGATATATCGCGACCGGCT GCGAATAAACAGATTTGCCTGGCCCAAGGTTCTAAAGATTTCATACAAACGGAACAACTTTTACATTAAG ATCCGGCCGGGAGAGTTTGAACAATTTGAAAGCACCATTGGGTTTAAGCTGCCAAACCATCGAGCTGCCA AGCGTTTATGGAAAGTATGTGTTGAGCATCATACATTTTTCAGACTACTGTTACCAGAAGCACCTCCCAA GAAATTCCTAACCTTGGGTTCCAAGTTTCGTTATAGTGGCAGGACACAAGCGCAAACGAGAAGAGCCAGT GCGTTGATAGATCGCCCAGCACCTTACTTTGAACGCTCATCCAGCAAACGTTATACCATGTCTCGCAGCT TGGATGGAGCATCAGTGAATGAAAACCATGAAATATACATGAAGGATTCTATGTCTGCTGCAGAGGTTGG TACTGGCCAGTACGCCACAACAAAAGGCATCTCTCAGACCAACTTGATCACCACTGTGACTCCGGAGAAG AAGGCTGAGGAGGAGCGGGACGAGGAAGAGGACAAACGGAGGAAGGGGGAAGAAGTCACGCCCATCTCGG CCATCCGGCACGAGGGAAAGACTGACAGTGAGCGCACGGACACCGCAGCCGACGGGGAGACCACTGCCAC TGAGTCGGACCAGGAGGAAGATGCAGAGCTCAAGGCACAGGAGCTAGAAAAAACTCAAGATGACCTGATG AAACATCAAACCAACATTAGCGAGCTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCGTAACGA ATGAATGGGAGAAGAGGCTTTCCACCTCCCCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGAT CGAACCACTTGTCCCTGAAGAGACTAAGCAGTCTTCTGGGGAAAAGCTCATGGATGGCTCTGAAATCTTC AGTTTATTAGAGTCTGCGCGAAAACCAACAGAATTCATAGGAGGGGTTACTTCTACTTCTCAAAGCTGGG TTCAGAAAATGGAAACCAAGACGGAGTCCAGTGGAATAGAGACGGAACCCACCGTGCACCACCTGCCGCT TAGCACTGAGAAGGTGGTGCAGGAGACCGTGTTGGTGGAGGAGCGGCGTGTGGTGCACGCGAGTGGGGAT GCTTCTTACTCGGCGGGAGACAGCGGGGATGCTGCAGCACAGCCCGCATTCACAGGCATTAAAGGGAAAG AGGGCTCTGCCTTGACGGAGGGGGCTAAAGAGGAAGGAGGGGAGGAGGTCGCTAAAGCTGTCCTGGAACA GGAAGAGACAGCCGCTGCTTCCCGTGAGCGACAAGAGGAGCAGAGTGCAGCCATCCACATTTCAGAAACT TTGGAACAAAAACCTCATTTTGAGTCCTCAACGGTGAAGACGGAAACCATCAGTTTTGGCAGTGTTTCAC CGGGAGGAGTAAAGCTAGAAATTTCCACGAAGGAAGTGCCAGTAGTTCACACCGAAACCAAAACCATCAC ATATGAATCATCACAGGTCGATCCAGGCACAGATCTGGAGCCAGGCGTGCTGATGAGTGCACAGACGATC ACATCTGAAACCACCAGTACCACCACCACTACGCACATCACCAAAACTGTGAAAGGGGGCATTTCAGAGA CAAGAATTGAGAAGCGAATAGTCATCACGGGGGATGCAGACATTGACCATGACCAGGAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281533
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281533.1, NP_001268462.1
RefSeq Size	3972 bp
RefSeq ORF	2652 bp
Locus ID	23136
UniProt ID	Q9Y2J2
Protein Pathways	Tight junction
Gene Summary	Tumor suppressor that inhibits cell proliferation and promotes apoptosis. Modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains a different 5' non-coding exon, uses an alternate in-frame acceptor splice site at an internal exon, and lacks 2 exons in the 3' coding region, the latter of which causes a frame-shift compared to variant 1. The resulting isoform (2) is shorter with a distinct C-terminus compared to isoform 1.

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