SH3D20 (ARHGAP27) (NM_001282290) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAMGAP1; PP905; SH3D20; SH3P20
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282290, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGGACGTGGTGGGGGACGTGTACGTGCTGGTGGAGCACCCCTTCGAGTACACCGGCAAGGACG GGCGCCGCGTGGCCATCCGGCCGAATGAGCGCTACCGGCTGCTGCGGCGCAGCACCGAGCACTGGTGGCA CGTGCGGCGTGAGCCCGGCGGCCGCCCCTTCTACCTGCCTGCGCAGTACGTGCGCGAGCTGCCCGCGCTG GGCAACCCTGCCGCCGCCGCGCCGCCAGGTCCCCACCCGAGCCCCGCGGCCCCTGAGCCGCTCGCCTACG ACTACCGGTTTGTGAGCGCGGCGGCGACCGCGGGCCCCGACGGCGCCCCCGAGGAGTCCGGAGGCCGAGC CAGCTCCCTGTGCGGCCCTGCGCAACGCGGCGCCGCGACCCAGCGCAGCAGCCTGGCGCCCGGCCTGCCA GCCTGCCTGTACCTGCGGCCCGCGGCGCCCGTGCGGCCCGCGCAGTCCCTGAACGACCTGGCCTGCGCCG CCGTCTCGCCTCCCGCCGGCCTCCTAGGAAGCAGCGGCAGCTTCAAGGCCTGCAGCGTGGCGGGCTCCTG GGTGTGCCCGCGGCCTCTGGCGCGCAGCGACTCAGAGAACGTCTACGAGGTCATCCAGGACTTGCACGTC CCGCCGCCGGAGGAGAGCGCAGAGCAGGTGGACGACCCACCGGAGCCCGTGTACGCGAACATAGAGAGGC AGCCCCGGGCCACTTCACCGGGCGCCGCTGCAGCCCCCCTTCCCAGCCCGGTGTGGGAGACGCACACGGA CGCGGGCACCGGGCGCCCCTACTACTACAACCCAGACACGGGAGTTACCACCTGGGAGTCGCCCTTTGAG GCTGCCGAGGGTGCCGCCAGCCCAGCCACCTCCCCTGCCTCGGTGGACAGCCACGTGAGCCTTGAGACCG AGTGGGGCCAGTACTGGGATGAGGAGAGCCGCAGGGTGTTCTTCTACAACCCGCTGACGGGCGAGACGGC CTGGGAGGACGAGGCCGAGAACGAGCCCGAGGAGGAGTTGGAGATGCAGCCGGGCCTGAGCCCTGGCAGC CCAGGGGACCCGCGGCCCCCCACTCCCGAGACGGACTACCCCGAGTCGCTGACCAGTTACCCCGAGGAGG ACTATTCTCCCGTGGGCTCTTTCGGTGAGCCCGGCCCTACCTCTCCCTTGACCACACCCCCCGGCTGGTC TTGTCATGTCAGCCAGGACAAGCAGATGCTCTACACCAACCACTTCACTCAGGAGCAGTGGGTGAGGCTG GAGGACCCCCACGGGAAGCCATACTTCTACAATCCAGAGGACTCCTCTGTTCGATGGGAGCTGCCCCAGG TCCCTGTCCCTGCCCCTCGAAGCATCCATAAATCCAGCCAGGATGGTGACACCCCAGCCCAGGCCAGCCC TCCAGAGGAGAAGGTCCCAGCAGAGCTGGATGAGGTTGGGAGCTGGGAGGAAGTCTCTCCTGCCACAGCT GCTGTGAGGACCAAGACCTTGGACAAGGCAGGGGTGCTCCATCGCACCAAGACGGCAGACAAGGGAAAGC GGCTCCGGAAGAAGCACTGGAGTGCCTCCTGGACTGTGCTGGAGGGTGGCGTCCTGACATTCTTCAAGGA CTCAAAGACCTCGGCTGCAGGCGGCCTGAGGCAGCCTTCCAAGTTTTCCACCCCTGAGTACACAGTGGAG CTGAGGGGGGCCACTCTCTCCTGGGCCCCCAAAGACAAATCCAGTAGGAAGAATGTGCTGGAGCTACGGA GCCGAGATGGCTCTGAGTACCTGATCCAGCACGACTCGGAGGCCATCATCAGCACCTGGCATAAGGCCAT TGCTCAGGGCATCCAGGAGCTGTCCGCAGAGCTGCCCCCAGAGGAGAGCGAGAGCAGCAGAGTGGACTTC GGGTCGAGCGAGCGCTTGGGAAGCTGGCAGGAGAAAGAGGAGGACGCGCGACCGAATGCAGCCGCGCCCG CCCTGGGCCCCGTGGGCCTGGAGAGCGACTTGAGCAAGGTCCGGCACAAGCTCCGCAAGTTCCTCCAGAG GCGGCCCACACTGCAGTCGCTGCGGGAGAAGGGCTACATCAAAGACCAGGTGTTCGGCTGCGCGCTGGCC GCGCTGTGTGAGCGCGAGAGGAGCCGGGTGCCACGCTTCGTGCAGCAGTGCATCCGCGCCGTCGAGGCCC GCGGGCTGGACATCGACGGGCTGTACCGCATCAGTGGAAACCTGGCCACCATCCAGAAGCTACGCTATAA GGTGGACCACGATGAGCGCCTTGACCTGGATGACGGGCGCTGGGAGGACGTCCACGTTATCACCGGAGCC CTGAAGCTCTTCTTTCGGGAGCTGCCCGAGCCCCTCTTCCCCTTCTCGCACTTCCGCCAGTTCATTGCGG CCATCAAGTTGCAGGACCAGGCCCGGCGCAGCCGCTGTGTGCGTGACTTGGTGCGCTCGCTGCCCGCTCC CAACCACGACACTCTGCGGATGCTCTTCCAGCACCTCTGCCGGGTGATCGAGCACGGCGAGCAGAACCGC ATGTCGGTGCAGAGCGTGGCCATTGTGTTCGGGCCCACGCTGCTGCGGCCCGAGGTGGAAGAGACCAGCA TGCCCATGACCATGGTGTTCCAGAACCAGGTGGTGGAGCTCATCCTGCAGCAGTGCGCGGACATCTTCCC GCCGCACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282290
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282290.1, NP_001269219.1
RefSeq Size	4242 bp
RefSeq ORF	2670 bp
Locus ID	201176
UniProt ID	Q6ZUM4
Gene Summary	This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein may pay a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (d). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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