Nephrocystin 4 (NPHP4) (NM_001291594) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	POC10; SLSN4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001291594, the custom clone sequence may differ by one or more nucleotides ATGCACCCACGGAGCCTGTCAGTGGCCCCAAAGTGGAGCGGCGGCCTTCCAGGAAACCACCCACGTCCCC TTCGACAGGAGTTCCCGTTGGAGGCCGGTATCTCCCACCTGGAAGCCGACCTGAGCCAGACCTCCCTGGT CCTGGAAACATCCATTGCCGAACAGTTACAGGAGCTGCCGTTCACGCCTTTGCATGCCCCTATTGTTGTG GGAACCCAGACCAGGAGCTCTGCAGGGCAGCCCTCGAGAGCCTCCATGGTGCTCCTGCAGTCCTCCGGCT TTCCCGAGATTCTGGATGCCAATAAACAGCCAGCCGAGGCTGTCAGCGCTACAGAACCTGTGACGTTTAA CCCTCAGAAGGAAGAATCAGATTGTCTACAAAGCAACGAGATGGTGCTACAGTTTCTTGCCTTTAGCAGA GTGGCCCAGGACTGCCGAGGAACATCATGGCCAAAGACTGTGTATTTCACCTTCCAGTTCTACCGCTTCC CACCCGCAACGACGCCACGACTGCAGCTGGTCCAGCTGGATGAGGCCGGCCAGCCCAGCTCTGGCGCCCT GACCCACATCCTCGTGCCTGTGAGCAGAGATGGCACCTTTGATGCTGGGTCTCCTGGCTTCCAGCTGAGG TACATGGTGGGCCCTGGGTTCCTGAAGCCAGGTGAGCGGCGCTGCTTTGCCCGCTACCTGGCCGTGCAGA CCCTGCAGATTGACGTCTGGGACGGAGACTCCCTGCTGCTCATCGGATCTGCTGCCGTCCAGATGAAGCA TCTCCTCCGCCAAGGCCGGCCGGCTGTGCAGGCCTCCCACGAGCTTGAGGTCGTGGCAACTGAATACGAG CAGGACAACATGGTGGTGAGTGGAGACATGCTGGGGTTTGGCCGCGTCAAGCCCATCGGCGTCCACTCGG TGGTGAAGGGCCGGCTGCACCTGACTTTGGCCAACGTGGGTCACCCGTGTGAACAGAAAGTGAGAGGTTG TAGCACATTGCCACCGTCCAGATCTCGGGTCATCTCAAACGATGGAGCCAGCCGCTTCTCTGGAGGCAGC CTCCTCACGACTGGAAGCTCAAGGCGAAAACACGTGGTGCAAGCACAGAAGCTGGCGGACGTGGACAGTG AGCTGGCTGCCATGCTACTGACCCATGCCCGGCAGGGCAAGGGGCCCCAGGACGTCAGCCGCGAGTCGGA TGCCACCCGCAGGCGTAAGCTGGAGCGGATGAGGTCTGTGCGCCTGCAGGAGGCCGGGGGAGACTTGGGC CGGCGCGGGACGAGCGTGTTGGCGCAGCAGAGCGTCCGCACACAGCACTTGCGGGACCTACAGGTCATCG CCGCCTACCGGGAACGCACGAAGGCCGAGAGCATCGCCAGCCTGCTGAGCCTGGCCATCACCACGGAGCA CACGCTCCACGCCACGCTGGGGGTCGCCGAGTTCTTTGAGTTTGTGCTTAAGAACCCCCACAACACACAG CACACGGTGACTGTGGAGATCGACAACCCCGAGCTCAGCGTCATCGTGGACAGTCAGGAGTGGAGGGACT TCAAGGGTGCTGCTGGCCTGCACACACCGGTGGAGGAGGACATGTTCCACCTGCGTGGCAGCCTGGCCCC CCAGCTCTACCTGCGCCCCCACGAGACCGCCCACGTCCCCTTCAAGTTCCAGAGCTTCTCTGCAGGGCAG CTGGCCATGGTGCAGGCCTCTCCTGGGTTGAGCAACGAGAAGGGCATGGACGCCGTGTCACCTTGGAAGT CCAGCGCAGTGCCCACTAAACACGCCAAGGTCTTGTTCCGAGCGAGTGGTGGCAAGCCCATCGCCGTGCT CTGCCTGACTGTGGAGCTGCAGCCCCACGTGGTGGACCAGGTCTTCCGCTTCTATCACCCGGAGCTCTCC TTCCTGAAGAAGGCCATCCGCCTGCCGCCCTGGCACACATTTCCAGGTGCTCCGGTGGGAATGCTTGGTG AGGACCCCCCAGTCCATGTTCGCTGCAGCGACCCGAACGTCATCTGTGAGACCCAGAATGTGGGCCCCGG GGAACCACGGGACATATTTCTGAAGGTGGCCAGTGGTCCAAGCCCGGAGATCAAAGACTTCTTTGTCATC ATTTACTCGGATCGCTGGCTGGCGACACCCACACAGACGTGGCAGGTCTACCTCCACTCCCTGCAGCGCG TGGATGTCTCCTGCGTCGCAGGCCAGCTGACCCGCCTGTCCCTTGTCCTTCGGGGGACACAGACAGTGAG GAAAGTGAGAGCTTTCACCTCTCATCCCCAGGAGCTGAAGACAGACCCCAAAGGTGTCTTCGTGCTGCCG CCTCGTGGGGTGCAGGACCTGCATGTTGGCGTGAGGCCCCTTAGGGCCGGCAGCCGCTTTGTCCATCTCA ACCTGGTGGACGTGGATTGCCACCAGCTGGTGGCCTCCTGGCTCGTGTGCCTCTGCTGCCGCCAGCCGCT CATCTCCAAGGCCTTTGAGATCATGTTGGCTGCGGGCGAAGGGAAGGGTGTCAACAAGAGGATCACCTAC ACCAACCCCTACCCCTCCCGGAGGACATTCCACCTGCACAGCGACCACCCGGAGCTGCTGCGGTTCAGAG AGGACTCCTTCCAGGTCGGGGGTGGAGAGACCTACACCATCGGCTTGCAGTTTGCGCCTAGTCAGAGAGT GGGTGAGGAGGAGATCCTGATCTACATCAATGACCATGAGGACAAAAACGAAGAGGCATTTTGCGTGAAG GTCATCTACCAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001291594
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291594.1, NP_001278523.1
RefSeq Size	4533 bp
RefSeq ORF	2745 bp
Locus ID	261734
UniProt ID	O75161
Gene Summary	This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (3) lacks four exons in the 5' region, and initiates translation at an alternate downstream start codon, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform 1.

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