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Nephrocystin 4 (NPHP4) (NM_001291594) Human Tagged ORF Clone
CAT＃: RG239776
- TrueORF®
NPHP4 (tGFP-tagged) - Human nephronophthisis 4 (NPHP4), transcript variant 3
ORF Plasmid: DDK
"NM_001291594" in other vectors (2)
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CNY 7,510.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | POC10; SLSN4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239776 representing NM_001291594.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCACCCACGGAGCCTGTCAGTGGCCCCAAAGTGGAGCGGCGGCCTTCCAGGAAACCACCCACGTCCC CTTCGACAGGAGTTCCCGTTGGAGGCCGGTATCTCCCACCTGGAAGCCGACCTGAGCCAGACCTCCCTG GTCCTGGAAACATCCATTGCCGAACAGTTACAGGAGCTGCCGTTCACGCCTTTGCATGCCCCTATTGTT GTGGGAACCCAGACCAGGAGCTCTGCAGGGCAGCCCTCGAGAGCCTCCATGGTGCTCCTGCAGTCCTCC GGCTTTCCCGAGATTCTGGATGCCAATAAACAGCCAGCCGAGGCTGTCAGCGCTACAGAACCTGTGACG TTTAACCCTCAGAAGGAAGAATCAGATTGTCTACAAAGCAACGAGATGGTGCTACAGTTTCTTGCCTTT AGCAGAGTGGCCCAGGACTGCCGAGGAACATCATGGCCAAAGACTGTGTATTTCACCTTCCAGTTCTAC CGCTTCCCACCCGCAACGACGCCACGACTGCAGCTGGTCCAGCTGGATGAGGCCGGCCAGCCCAGCTCT GGCGCCCTGACCCACATCCTCGTGCCTGTGAGCAGAGATGGCACCTTTGATGCTGGGTCTCCTGGCTTC CAGCTGAGGTACATGGTGGGCCCTGGGTTCCTGAAGCCAGGTGAGCGGCGCTGCTTTGCCCGCTACCTG GCCGTGCAGACCCTGCAGATTGACGTCTGGGACGGAGACTCCCTGCTGCTCATCGGATCTGCTGCCGTC CAGATGAAGCATCTCCTCCGCCAAGGCCGGCCGGCTGTGCAGGCCTCCCACGAGCTTGAGGTCGTGGCA ACTGAATACGAGCAGGACAACATGGTGGTGAGTGGAGACATGCTGGGGTTTGGCCGCGTCAAGCCCATC GGCGTCCACTCGGTGGTGAAGGGCCGGCTGCACCTGACTTTGGCCAACGTGGGTCACCCGTGTGAACAG AAAGTGAGAGGTTGTAGCACATTGCCACCGTCCAGATCTCGGGTCATCTCAAACGATGGAGCCAGCCGC TTCTCTGGAGGCAGCCTCCTCACGACTGGAAGCTCAAGGCGAAAACACGTGGTGCAAGCACAGAAGCTG GCGGACGTGGACAGTGAGCTGGCTGCCATGCTACTGACCCATGCCCGGCAGGGCAAGGGGCCCCAGGAC GTCAGCCGCGAGTCGGATGCCACCCGCAGGCGTAAGCTGGAGCGGATGAGGTCTGTGCGCCTGCAGGAG GCCGGGGGAGACTTGGGCCGGCGCGGGACGAGCGTGTTGGCGCAGCAGAGCGTCCGCACACAGCACTTG CGGGACCTACAGGTCATCGCCGCCTACCGGGAACGCACGAAGGCCGAGAGCATCGCCAGCCTGCTGAGC CTGGCCATCACCACGGAGCACACGCTCCACGCCACGCTGGGGGTCGCCGAGTTCTTTGAGTTTGTGCTT AAGAACCCCCACAACACACAGCACACGGTGACTGTGGAGATCGACAACCCCGAGCTCAGCGTCATCGTG GACAGTCAGGAGTGGAGGGACTTCAAGGGTGCTGCTGGCCTGCACACACCGGTGGAGGAGGACATGTTC CACCTGCGTGGCAGCCTGGCCCCCCAGCTCTACCTGCGCCCCCACGAGACCGCCCACGTCCCCTTCAAG TTCCAGAGCTTCTCTGCAGGGCAGCTGGCCATGGTGCAGGCCTCTCCTGGGTTGAGCAACGAGAAGGGC ATGGACGCCGTGTCACCTTGGAAGTCCAGCGCAGTGCCCACTAAACACGCCAAGGTCTTGTTCCGAGCG AGTGGTGGCAAGCCCATCGCCGTGCTCTGCCTGACTGTGGAGCTGCAGCCCCACGTGGTGGACCAGGTC TTCCGCTTCTATCACCCGGAGCTCTCCTTCCTGAAGAAGGCCATCCGCCTGCCGCCCTGGCACACATTT CCAGGTGCTCCGGTGGGAATGCTTGGTGAGGACCCCCCAGTCCATGTTCGCTGCAGCGACCCGAACGTC ATCTGTGAGACCCAGAATGTGGGCCCCGGGGAACCACGGGACATATTTCTGAAGGTGGCCAGTGGTCCA AGCCCGGAGATCAAAGACTTCTTTGTCATCATTTACTCGGATCGCTGGCTGGCGACACCCACACAGACG TGGCAGGTCTACCTCCACTCCCTGCAGCGCGTGGATGTCTCCTGCGTCGCAGGCCAGCTGACCCGCCTG TCCCTTGTCCTTCGGGGGACACAGACAGTGAGGAAAGTGAGAGCTTTCACCTCTCATCCCCAGGAGCTG AAGACAGACCCCAAAGGTGTCTTCGTGCTGCCGCCTCGTGGGGTGCAGGACCTGCATGTTGGCGTGAGG CCCCTTAGGGCCGGCAGCCGCTTTGTCCATCTCAACCTGGTGGACGTGGATTGCCACCAGCTGGTGGCC TCCTGGCTCGTGTGCCTCTGCTGCCGCCAGCCGCTCATCTCCAAGGCCTTTGAGATCATGTTGGCTGCG GGCGAAGGGAAGGGTGTCAACAAGAGGATCACCTACACCAACCCCTACCCCTCCCGGAGGACATTCCAC CTGCACAGCGACCACCCGGAGCTGCTGCGGTTCAGAGAGGACTCCTTCCAGGTCGGGGGTGGAGAGACC TACACCATCGGCTTGCAGTTTGCGCCTAGTCAGAGAGTGGGTGAGGAGGAGATCCTGATCTACATCAAT GACCATGAGGACAAAAACGAAGAGGCATTTTGCGTGAAGGTCATCTACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239776
Blue=ORF Red=Cloning site Green=Tag(s) MHPRSLSVAPKWSGGLPGNHPRPLRQEFPLEAGISHLEADLSQTSLVLETSIAEQLQELPFTPLHAPIV VGTQTRSSAGQPSRASMVLLQSSGFPEILDANKQPAEAVSATEPVTFNPQKEESDCLQSNEMVLQFLAF SRVAQDCRGTSWPKTVYFTFQFYRFPPATTPRLQLVQLDEAGQPSSGALTHILVPVSRDGTFDAGSPGF QLRYMVGPGFLKPGERRCFARYLAVQTLQIDVWDGDSLLLIGSAAVQMKHLLRQGRPAVQASHELEVVA TEYEQDNMVVSGDMLGFGRVKPIGVHSVVKGRLHLTLANVGHPCEQKVRGCSTLPPSRSRVISNDGASR FSGGSLLTTGSSRRKHVVQAQKLADVDSELAAMLLTHARQGKGPQDVSRESDATRRRKLERMRSVRLQE AGGDLGRRGTSVLAQQSVRTQHLRDLQVIAAYRERTKAESIASLLSLAITTEHTLHATLGVAEFFEFVL KNPHNTQHTVTVEIDNPELSVIVDSQEWRDFKGAAGLHTPVEEDMFHLRGSLAPQLYLRPHETAHVPFK FQSFSAGQLAMVQASPGLSNEKGMDAVSPWKSSAVPTKHAKVLFRASGGKPIAVLCLTVELQPHVVDQV FRFYHPELSFLKKAIRLPPWHTFPGAPVGMLGEDPPVHVRCSDPNVICETQNVGPGEPRDIFLKVASGP SPEIKDFFVIIYSDRWLATPTQTWQVYLHSLQRVDVSCVAGQLTRLSLVLRGTQTVRKVRAFTSHPQEL KTDPKGVFVLPPRGVQDLHVGVRPLRAGSRFVHLNLVDVDCHQLVASWLVCLCCRQPLISKAFEIMLAA GEGKGVNKRITYTNPYPSRRTFHLHSDHPELLRFREDSFQVGGGETYTIGLQFAPSQRVGEEEILIYIN DHEDKNEEAFCVKVIYQ TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001291594 |
| ORF Size | 2742 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001291594.2 |
| RefSeq Size | 4533 bp |
| RefSeq ORF | 2745 bp |
| Locus ID | 261734 |
| UniProt ID | O75161 |
| MW | 101.5 kDa |
| Gene Summary | This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
Documents
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| SDS |
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