Pumilio 2 (PUM2) (NM_001282752) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PUMH2; PUML2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282752, the custom clone sequence may differ by one or more nucleotides ATGTCCCAGCCTATTATGGTACAGAGAAGATCTGGACAGGGTTTTCATGGAAACAGTGAAGTAAATGCAA TACTGTCTCCGCGATCAGAAAGTGGAGGCCTTGGTGTGAGCATGGTAGAATATGTATTAAGTTCTTCTCC TGCTGATAAATTGGATTCTCGATTTAGGAAGGGAAATTTTGGCACTAGAGATGCTGAAACAGATGGACCT GAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAGGACCAAAACAGAGATCTTAAACAAGGAG ATGATGATGATTCTAAAATAAATGGCAGAGGTTTGCCAAATGGAATGGATGCCGATTGCAAAGATTTTAA TCGTACTCCTGGAAGTCGTCAAGCCTCTCCAACTGAAGTAGTTGAGCGCTTGGGCCCCAATACTAATCCC TCAGAAGGACTGGGGCCTCTTCCTAATCCTACAGCTAATAAACCACTTGTTGAAGAATTTTCAAATCCTG AAACTCAGAATCTGGATGCCATGGAACAAGTTGGTCTGGAATCCTTACAGTTTGACTATCCTGGTAATCA GGTACCAATGGACTCTTCAGGAGCTACTGTAGGCCTTTTTGACTACAATTCCCAGCAGCAGCTCTTTCAG AGGACTAATGCACTAACAGTTCAACAGTTAACTGCAGCTCAACAGCAGCAATATGCATTAGCAGCAGCTC AGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTCCAGCTGCATTTGTGCCAAATCCATACAT TATTAGTGCTGCTCCTCCAGGGACCGATCCGTATACTGCAGCAGGATTGGCTGCAGCAGCTACATTAGCA GGTCCAGCAGTGGTTCCACCTCAGTATTACGGCGTTCCATGGGGGGTGTATCCAGCCAACTTATTTCAGC AGCAAGCTGCAGCTGCGGCAAATAACACAGCCAGTCAGCAAGCAGCATCACAAGCTCAGCCTGGACAGCA ACAGGTTCTCCGTGCTGGAGCAGGTCAGCGTCCTCTTACTCCCAATCAGGGTCAGCAAGGGCAGCAAGCA GAATCACTTGCGGCAGCTGCAGCAGCAAATCCAACATTGGCTTTTGGTCAGGGTCTTGCTACTGGCATGC CAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGACTGGTGCCTTAGTGGTTGGCCCTGGAGC AAGGACTGGCCTTGGAGCTCCAGTTCGGTTAATGGCTCCAACACCTGTTTTAATTAGTTCAGCAGCAGCA CAAGCTGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAGTAGCCTTACAGGCAGCACAAATGGTCTGTTTC GGCCAATTGGCACTCAGCCACCACAGCAGCAGCAACAGCAGCCAAGCACTAATCTGCAATCTAATTCATT TTATGGAAGCAGTTCTTTGACTAATAGCTCCCAGAGTAGTTCTTTATTTTCTCATGGACCTGGTCAACCT GGAAGTACATCTCTTGGCTTTGGAAGTGGTAACTCTTTGGGTGCTGCTATAGGCTCAGCCCTCAGTGGAT TTGGTTCATCAGTTGGCAGTTCTGCAAGTAGTAGTGCCACAAGGAGAGAGTCTCTATCTACTAGCTCTGA CTTGTACAAAAGATCTAGTAGCAGCCTAGCACCCATAGGGCAACCATTTTACAATAGTCTGGGATTTTCC TCCTCTCCAAGTCCAATAGGCATGCCTCTGCCAAGCCAAACTCCAGGACATTCACTTACGCCACCGCCAT CACTTTCATCACATGGATCCTCATCCAGTTTGCATTTAGGAGGACTGACAAATGGTAGTGGTCGATATAT CTCTGCAGCACCTGGAGCAGAAGCAAAATATCGAAGTGCTTCAAGCACTTCCAGTCTATTTAGCTCCAGC AGCCAGCTCTTTCCTCCTTCCCGGCTTCGGTATAATAGGTCTGATATTATGCCTTCTGGCCGCAGTAGAT TATTGGAAGATTTCAGAAACAACCGCTTCCCAAACCTTCAGCTTAGAGACTTGATTGGACATATAGTTGA GTTTTCTCAAGACCAGCATGGTTCTAGATTCATACAGCAAAAACTAGAGAGAGCTACTCCAGCTGAGCGA CAGATGGTATTTAATGAAATTCTGCAAGCAGCCTATCAATTAATGACTGATGTTTTTGGCAACTATGTTA TACAGAAGTTTTTTGAGTTTGGGAGTCTGGATCAAAAATTAGCCCTGGCTACTCGTATTCGTGGTCATGT TCTACCCTTAGCCTTGCAGATGTATGGCTGCCGCGTTATTCAGAAAGCATTAGAATCTATTTCTTCTGAC CAGCAGAGTGAAATGGTAAAGGAGCTGGATGGTCATGTGCTCAAATGTGTGAAAGATCAGAATGGAAACC ATGTTGTACAAAAATGTATCGAATGTGTTCAGCCACAGTCACTACAGTTCATCATTGATGCTTTCAAGGG ACAAGTATTTGTGCTTTCAACTCATCCTTATGGCTGCAGAGTAATTCAGCGCATCCTAGAGCATTGCACT GCAGAACAGACCTTACCTATCTTAGAAGAACTCCACCAACATACAGAGCAGTTGGTACAGGATCAGTATG GCAATTATGTTATTCAGCATGTACTGGAACACGGTCGACCTGAAGACAAGAGCAAAATTGTTTCCGAAAT CAGGGGAAAGGTTTTAGCCCTGAGTCAACACAAATTTGCCAGCAATGTAGTAGAAAAGTGTGTTACTCAT GCCTCCCGTGCTGAGAGAGCTTTACTGATTGACGAGGTTTGCTGCCAGAATGATGGTCCTCACAGTGCCT TATACACCATGATGAAGGACCAGTATGCCAATTACGTGGTTCAAAAGATGATTGATATGGCTGAACCTGC TCAGAGAAAGATAATCATGCACAAGATTCGACCTCACATTACTACTTTGCGCAAATACACATACGGGAAG CATATACTGGCCAAGTTGGAAAAGTATTATTTGAAGAATAGCCCGGACCTAGGACCTATTGGAGGACCAC CAAATGGAATGCTGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282752
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282752.1, NP_001269681.1
RefSeq Size	6251 bp
RefSeq ORF	3027 bp
Locus ID	23369
UniProt ID	Q8TB72
Gene Summary	This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.