ERC1 (NM_001301248) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Cast2; ELKS; ERC-1; RAB6IP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301248, the custom clone sequence may differ by one or more nucleotides ATGTATGGAAGTGCCCGCTCTGTTGGGAAGGTGGAGCCGAGCAGCCAGAGCCCTGGGCGTTCACCCAGGC TTCCACGTTCCCCTCGCTTGGGTCACCGTCGAACCAACAGTACGGGAGGGAGTTCGGGAAGCAGTGTTGG AGGTGGCAGTGGGAAAACCCTTTCAATGGAAAATATACAATCTTTAAATGCTGCCTATGCCACCTCTGGC CCTATGTATCTAAGTGACCATGAAAATGTGGGTTCAGAAACACCTAAAAGCACCATGACACTTGGCCGTT CTGGGGGACGTCTGCCTTACGGTGTTCGGATGACTGCTATGGGTAGTAGCCCCAATATAGCTAGCAGTGG GGTTGCTAGTGACACCATAGCATTTGGAGAGCATCACCTCCCTCCTGTGAGTATGGCATCCACTGTACCT CACTCCCTTCGTCAGGCGAGAGATAACACAATCATGGATCTGCAGACACAGCTGAAGGAAGTATTAAGAG AAAATGATCTCTTGCGGAAGGATGTGGAAGTAAAGGAGAGCAAATTGAGTTCTTCAATGAATAGCATCAA GACCTTCTGGAGCCCAGAGCTGAAGAAGGAACGAGCCCTGAGAAAAGATGAAGCTTCCAAAATCACCATT TGGAAGGAACAGTACAGAGTTGTACAGGAGGAAAACCAGCACATGCAGATGACAATCCAGGCTCTCCAGG ATGAATTGCGGATCCAGAGGGACCTGAATCAGCTGTTTCAGCAGGATAGTAGCAGCAGGACTGGCGAACC TTGTGTAGCAGAGCTGACAGAGGAGAACTTTCAGAGGCTTCATGCTGAGCATGAGCGGCAGGCCAAAGAG CTGTTTCTTCTTCGAAAGACATTGGAGGAAATGGAGCTGCGTATTGAGACTCAAAAGCAGACCCTAAATG CTCGGGATGAATCCATTAAGAAGCTTCTGGAAATGTTGCAGAGCAAAGGACTTTCTGCCAAGGCTACCGA GGAAGACCATGAGAGAACAAGACGACTGGCAGAGGCAGAGATGCACGTTCATCACCTAGAAAGCCTTTTG GAGCAGAAGGAAAAAGAGAACAGTATGTTGAGAGAGGAGATGCATCGAAGGTTTGAGAATGCTCCTGATT CTGCCAAAACAAAAGCTCTGCAAACTGTTATTGAGATGAAGGATTCAAAAATTTCCTCTATGGAGCGTGG GCTTCGAGACCTGGAAGAGGAAATTCAGATGCTGAAATCGAATGGTGCTTTGAGTACTGAGGAAAGGGAA GAAGAAATGAAGCAAATGGAAGTGTATCGGAGCCATTCTAAATTTATGAAAAATAAGGTAGAACAACTGA AGGAGGAACTAAGTTCGAAAGAGGCTCAATGGGAGGAGCTGAAAAAGAAAGCGGCTGGTCTTCAGGCTGA GATTGGCCAGGTGAAACAGGAGCTGTCCAGAAAGGACACAGAACTACTCGCCCTGCAGACAAAGCTAGAA ACACTCACAAACCAGTTCTCAGATAGTAAACAGCACATTGAAGTGTTGAAGGAGTCCTTGACTGCTAAGG AGCAGAGGGCTGCCATCCTGCAGACTGAGGTGGATGCTCTCCGATTGCGTTTGGAAGAGAAGGAAACCAT GTTGAATAAAAAGACAAAACAAATTCAGGATATGGCTGAAGAGAAGGGGACACAAGCTGGAGAGATACAT GACCTCAAGGACATGTTGGATGTGAAGGAGCGGAAGGTTAATGTTCTTCAGAAGAAGATTGAAAATCTTC AAGAGCAGCTTAGAGACAAGGAAAAGCAGATGAGCAGCTTGAAAGAACGGGTCAAATCCTTGCAGGCTGA CACCACCAACACTGACACTGCCTTGACAACTTTGGAGGAGGCCCTTGCAGAGAAAGAGCGGACAATTGAA CGCTTAAAGGAGCAGAGGGACAGAGATGAGCGAGAGAAGCAAGAGGAAATTGATAACTACAAAAAAGATC TTAAAGACTTGAAGGAAAAAGTCAGCCTGTTGCAAGGCGACCTTTCAGAGAAAGAGGCTTCACTTTTGGA TCTGAAAGAGCATGCTTCTTCTCTGGCATCCTCAGGACTGAAAAAGGACTCACGGCTTAAGACACTAGAG ATTGCTTTGGAGCAGAAGAAGGAGGAGTGTCTGAAAATGGAATCACAATTGAAAAAGGCACATGAGGCAG CATTGGAAGCCAGAGCCAGTCCAGAGATGAGTGACCGAATACAGCACTTGGAGAGAGAGATCACCAGGTA CAAAGATGAATCTAGCAAGGCCCAGGCAGAAGTTGATCGACTCTTAGAAATCTTGAAGGAGGTGGAAAAT GAGAAGAATGACAAAGATAAGAAGATAGCTGAGTTGGAAAGGCAAGTGAAAGACCAGAATAAGAAGGTAG CAAATCTGAAGCACAAGGAACAGGTGGAAAAAAAGAAGAGTGCACAAATGTTAGAGGAGGCGCGACGACG GGAGGACAATCTCAACGACAGCTCTCAGCAGCTACAGGACAGTCTCCGTAAGAAGGATGACAGGATTGAA GAGCTGGAAGAAGCACTAAGAGAAAGTGTACAGATAACTGCAGAGCGGGAAATGGTGCTAGCACAAGAGG AATCAGCCAGGACCAATGCTGAAAAACAGGTGGAGGAGTTACTGATGGCCATGGAGAAGGTAAAGCAGGA ACTAGAATCCATGAAAGCAAAGCTGTCCTCCACCCAGCAGTCTCTGGCAGAAAAGGAAACTCACTTGACT AATCTTCGGGCAGAGAGAAGGAAACACTTAGAGGAAGTTCTGGAGATGAAGCAAGAAGCTCTTCTGGCTG CCATTAGTGAAAAAGACGCCAATATAGCTCTCTTGGAGCTTTCGTCCTCTAAGAAGAAGACCCAAGAGGA AGTGGCTGCCCTGAAGCGGGAGAAGGATCGTCTGGTACAGCAGCTTAAGCAGCAGACGCAAAATCGAATG AAGCTAATGGCCGACAACTACGAGGATGACCACTTCAAATCCTCCCATTCCAATCAAACAAATCACAAGC CCTCCCCAGACCAGATCATCCAGCCCCTCTTAGAACTTGACCAAAATAGAAGTAAATTAAAGTTGTACAT TGGACACCTGACAACCCTCTGCCATGACCGAGACCCCCTGATCCTCCGTGGACTCACTCCACCAGCTTCC TATAACTTGGACGATGACCAGGCGGCTTGGGAGAATGAGCTGCAGAAGATGACCCGGGGGCAGAATCCGT TGCATCCCAAAGCCATGGAAAAACTGCCTTTTTTCATGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001301248
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301248.1, NP_001288177.1
RefSeq Size	9284 bp
RefSeq ORF	3261 bp
Locus ID	23085
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene is a member of a family of RIM-binding proteins. RIMs are active zone proteins that regulate neurotransmitter release. This gene has been found fused to the receptor-type tyrosine kinase gene RET by gene rearrangement due to the translocation t(10;12)(q11;p13) in thyroid papillary carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (zeta) contains an additional exon that results in an alternate 3' coding region and 3' UTR, compared to variant epsilon. The encoded isoform (zeta) has a distinct C-terminus and is shorter than isoform epsilon. The 5' UTR of this variant is incomplete due to lack of full-length transcript support and splice site ambiguity in the 5'-most exon. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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