BAI2 (ADGRB2) (NM_001294336) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BAI2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001294336, the custom clone sequence may differ by one or more nucleotides ATGGAGAATACAGGTTGGATGGGCAAGGGACATAGGATGACCCCAGCCTGTCCCCTCTTACTGTCTGTGA TTCTGTCCCTGCGCCTGGCCACCGCCTTCGACCCCGCCCCCAGTGCCTGCTCTGCCCTGGCCTCGGGTGT GCTCTACGGGGCCTTCTCGCTGCAGGACCTCTTTCCTACCATCGCCTCGGGCTGCTCCTGGACCCTGGAG AACCCTGACCCCACCAAGTACTCCCTCTACCTGCGCTTCAACCGCCAGGAGCAGGTGTGCGCACACTTTG CCCCCCGCCTGCTGCCCCTGGACCACTACCTGGTCAACTTTACCTGCCTGCGGCCTAGCCCCGAGGAGGC GGTGGCCCAGGCGGAGTCAGAGGTGGGGCGGCCAGAAGAGGAGGAGGCAGAGGCGGCAGCGGGGTTGGAG CTGTGCAGCGGCTCAGGCCCCTTTACCTTCCTGCACTTCGACAAGAACTTCGTGCAGCTGTGCCTGTCGG CTGAGCCCTCCGAGGCCCCGCGCCTGCTGGCGCCCGCTGCCCTAGCCTTCCGCTTTGTCGAGGTCTTGCT CATCAACAACAACAACTCTAGCCAATTCACCTGTGGTGTGCTCTGCCGCTGGAGTGAGGAGTGTGGCCGC GCTGCCGGCAGGGCCTGCGGCTTTGCTCAGCCAGGCTGCAGCTGCCCTGGAGAGGCGGGGGCCGGCTCCA CCACCACCACATCTCCAGGCCCTCCTGCTGCCCACACCCTGTCCAATGCCCTGGTGCCCGGGGGCCCAGC CCCACCTGCTGAGGCCGATTTGCACTCGGGGAGCAGCAATGATCTGTTCACAACCGAGATGAGATATGGT GAGGAGCCGGAAGAGGAACCGAAAGTGAAAACCCAGTGGCCGAGGTCTGCAGATGAGCCTGGGCTATACA TGGCGCAGACAGGCGACCCGGCGGCTGAGGAGTGGTCCCCGTGGAGCGTGTGTTCCCTGACGTGTGGGCA GGGTCTGCAGGTGCGGACCCGCTCCTGTGTGTCCTCCCCCTATGGGACCCTGTGCAGCGGGCCCCTGCGG GAGACCAGGCCCTGCAACAATTCAGCCACCTGCCCAGTGCACGGCGTGTGGGAGGAGTGGGGGTCCTGGA GCCTGTGCTCCCGCAGCTGCGGGCGGGGGTCCCGGAGCCGGATGCGGACCTGCGTGCCCCCCCAGCACGG CGGCAAGGCCTGCGAGGGTCCTGAGCTGCAGACTAAGCTCTGCAGTATGGCTGCCTGCCCGGTGGAAGGC CAGTGGTTAGAATGGGGTCCCTGGGGCCCATGCTCCACGTCCTGTGCCAATGGGACCCAACAGCGCAGCC GGAAGTGCAGCGTGGCGGGCCCAGCCTGGGCCACATGCACGGGTGCCCTCACTGACACCCGGGAGTGCAG CAACCTCGAGTGCCCGGCCACTGATAGCAAGTGGGGGCCATGGAATGCGTGGAGCCTGTGCTCTAAGACG TGTGACACAGGCTGGCAGCGCCGCTTCCGCATGTGCCAGGCCACGGGCACGCAGGGCTACCCCTGCGAGG GCACCGGAGAGGAGGTGAAGCCTTGTAGTGAGAAGAGGTGTCCAGCCTTCCATGAGATGTGCAGGGATGA GTACGTGATGCTGATGACGTGGAAGAAGGCAGCTGCTGGCGAGATCATCTACAACAAGTGCCCCCCGAAT GCCTCAGGGTCTGCCAGCCGCCGCTGTCTCCTCAGTGCCCAAGGCGTGGCGTACTGGGGGCTGCCCAGCT TTGCTCGCTGCATCTCCCATGAGTACCGCTACCTGTATCTGTCACTTAGGGAGCACCTGGCCAAGGGGCA GCGCATGCTGGCAGGCGAGGGCATGTCGCAGGTGGTGCGCAGCCTGCAGGAGCTACTGGCCCGGCGCACC TACTATAGTGGGGACCTGCTCTTCTCTGTGGACATTCTGAGGAATGTCACTGACACCTTTAAGAGGGCCA CCTACGTGCCCTCGGCTGATGATGTGCAGCGCTTCTTCCAGGTGGTGAGCTTCATGGTGGATGCGGAAAA CAAGGAGAAGTGGGACGATGCTCAGCAGGTGTCCCCTGGCTCTGTGCACCTGCTCCGTGTCGTGGAGGAC TTCATTCACCTGGTGGGCGATGCTCTCAAGGCCTTCCAGAGCTCTCTGATTGTCACAGATAATCTAGTGA TCAGCATTCAGCGAGAGCCCGTCTCAGCTGTGTCCAGTGACATCACGTTCCCCATGCGGGGCCGCCGGGG CATGAAGGACTGGGTGCGGCACTCAGAGGACCGCCTCTTCCTGCCCAAGGAGGTGCTCAGCCTCTCCTCC CCAGGGAAGCCAGCCACATCTGGGGCAGCAGGCAGCCCTGGCAGGGGGAGGGGCCCAGGAACGGTGCCTC CTGGCCCAGGCCACTCCCACCAGCGCCTCCTCCCAGCAGACCCTGATGAGTCCTCCTACTTTGTGATCGG TGCTGTACTCTACCGCACCCTTGGCCTCATCCTGCCGCCTCCCAGGCCCCCGCTGGCCGTCACATCCCGG GTGATGACAGTGACTGTGCGCCCCCCTACCCAGCCTCCAGCTGAGCCCCTCATCACTGTGGAGCTCTCCT ACATCATCAATGGGACCACGGATCCCCATTGCGCCAGCTGGGACTACTCCAGAGCAGATGCCAGCTCAGG AGACTGGGACACTGAAAATTGCCAGACCCTGGAGACCCAGGCAGCTCACACCCGCTGCCAGTGCCAGCAC CTGTCCACCTTTGCTGTACTAGCCCAGCCGCCCAAGGACCTGACCCTGGAGCTGGCGGGCTCCCCCTCGG TCCCCCTGGTGATCGGCTGTGCAGTGTCGTGCATGGCGCTGCTCACCCTGCTCGCCATCTATGCCGCCTT TTGGAGGTTCATAAAATCTGAACGCTCCATCATCTTGCTGAACTTCTGCCTGTCCATCTTGGCATCCAAC ATCCTGATCCTCGTGGGCCAGTCCCGGGTGCTGAGCAAGGGCGTGTGCACCATGACGGCTGCCTTCCTGC ACTTCTTCTTTCTCTCCTCCTTTTGCTGGGTGCTTACCGAGGCCTGGCAGTCCTACCTGGCTGTCATTGG GCGGATGCGCACCCGCCTCGTTCGCAAGCGCTTCCTCTGCCTGGGCTGGGGTCTGCCTGCCCTGGTGGTG GCCGTGTCTGTTGGCTTTACCCGAACGAAAGGATACGGTACATCCAGCTACTGCTGGCTCTCCCTGGAGG GCGGCCTGCTCTACGCCTTTGTGGGCCCTGCAGCCGTCATTGTCCTGGTGAACATGCTCATCGGAATCAT CGTCTTCAACAAGCTCATGGCACGTGATGGCATCTCCGACAAATCCAAGAAGCAGAGGGCCGGGGCCTCA CTCTGGAGCTCCTGCGTGGTGCTGCCCCTGCTGGCGCTCACCTGGATGTCTGCCGTCCTGGCTATGACAG ACCGCCGTTCCGTCCTCTTCCAGGCCCTCTTTGCTGTCTTCAACTCCGCGCAGGGCTTTGTCATCACTGC TGTGCACTGCTTCCTGCGCCGAGAGGTCCAGGATGTGGTGAAGTGCCAGATGGGGGTGTGCCGGGCTGAT GAGAGCGAAGACTCCCCTGACTCGTGTAAGAACGGGCAGCTGCAGATCCTGTCAGACTTTGAAAAGGATG TGGATCTGGCTTGTCAAACAGTGCTGTTCAAGGAGGTCAACACTTGCAACCCGTCCACCATCACGGGCAC ACTATCCCGCCTGTCCCTGGATGAGGATGAGGAGCCCAAGTCCTGCCTCGTGGGCCCTGAGGGCAGCCTC AGCTTCTCACCACTGCCTGGGAATATCCTGGTGCCCATGGCAGCCTCACCAGGGCTGGGGGAGCCTCCGC CCCCACAGGAGGCCAACCCTGTTTACATGTGTGGGGAGGGTGGCCTGCGGCAGCTGGACCTCACATGGCT GCGGCCCACTGAGCCAGGCTCTGAGGGAGACTACATGGTGCTGCCCCGGCGGACTTTGAGCCTGCAGCCT GGCGGTGGGGGTGGAGGTGGTGAGGATGCCCCCAGGGCCCGGCCGGAGGGGACCCCCCGGCGAGCTGCCA AGACAGTGGCCCACACTGAAGGCTACCCCAGCTTCCTGTCCGTGGACCACTCGGGCCTGGGGCTGGGCCC TGCCTATGGATCTCTCCAGAATCCCTATGGAATGACCTTCCAACCGCCACCGCCGACACCCAGCGCCCGC CAAGTGCCCGAGCCAGGGGAGCGCAGCCGGACCATGCCTCGCACCGTGCCCGGCTCTACCATGAAGATGG GCTCCCTGGAGCGAAAGAAATTACGGTATTCAGACCTGGACTTTGAGGTGATGCACACCCGGAAACGGCA TTCAGAACTCTACCACGAGCTCAACCAGAAGTTCCACACTTTCGACCGCTACCGCAGCCAGTCCACGGCC AAGAGGGAGAAGCGGTGGAGTGTGTCCTCGGGTGGGGCAGCCGAGCGGAGCGTGTGCACCGATAAGCCCA GCCCTGGGGAGCGCCCCAGCTTGTCCCAACATCGGCGCCATCAGAGCTGGAGCACCTTCAAATCTATGAC ACTGGGCTCGCTGCCCCCCAAGCCCCGAGAACGGCTGACTCTGCACCGGGCAGCAGCCTGGGAGCCCACA GAACCACCGGATGGTGACTTCCAGACAGAGGTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001294336
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001294336.1, NP_001281265.1
RefSeq Size	5356 bp
RefSeq ORF	4656 bp
Locus ID	576
UniProt ID	O60241
Protein Families	Druggable Genome, Transmembrane
Gene Summary	This gene encodes a a seven-span transmembrane protein that is thought to be a member of the secretin receptor family. The encoded protein is a brain-specific inhibitor of angiogenesis. The mature peptide may be further cleaved into additional products (PMID:20367554). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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