Collagen IV alpha 6 (COL4A6) (NM_001287759) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CXDELq22.3; DELXq22.3; DFNX6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001287759, the custom clone sequence may differ by one or more nucleotides ATGCACCCTGGGTTGTGGCTGCTCCTGGTTACGTTGTGCCTGACCGAGGAACTGGCAGCAGCGGGAGAGA AGTCTTATGGAAAGCCATGTGGGGGCCAGGACTGCAGTGGGAGCTGTCAGTGTTTTCCTGAGAAAGGAGC GAGAGGACGACCTGGACCAATTGGAATTCAAGGCCCAACAGGTCCTCAAGGATTCACTGGCTCTACTGGT TTATCGGGATTGAAAGGAGAAAGGGGTTTCCCAGGCCTTCTGGGACCTTATGGACCAAAAGGAGATAAGG GTCCCATGGGAGTTCCTGGCTTTCTTGGCATCAATGGGATTCCGGGCCACCCTGGACAACCAGGCCCCAG AGGCCCACCTGGTCTGGATGGCTGTAATGGAACTCAAGGAGCTGTTGGATTTCCAGGCCCTGATGGCTAT CCTGGGCTTCTCGGACCACCCGGGCTTCCTGGTCAGAAAGGATCAAAAGGTGACCCTGTCCTTGCTCCAG GTAGTTTCAAAGGAATGAAGGGGGATCCTGGGCTGCCTGGACTGGATGGAATCACTGGCCCACAAGGAGC ACCCGGATTTCCTGGAGCTGTAGGACCTGCAGGACCACCAGGATTACAAGGTCCTCCAGGGCCTCCTGGT CCTCTTGGTCCTGATGGGAATATGGGGCTAGGTTTTCAAGGAGAGAAAGGAGTCAAGGGGGATGTTGGCC TCCCTGGCCCAGCAGGACCTCCACCATCTACTGGAGAGCTGGAATTCATGGGATTCCCCAAAGGGAAGAA AGGATCCAAGGGTGAACCAGGGCCTAAGGGTTTTCCAGGCATAAGTGGCCCTCCAGGCTTCCCGGGCCTT GGAACTACTGGAGAAAAGGGAGAAAAGGGAGAAAAGGGAATCCCTGGTTTGCCAGGACCTAGGGGTCCCA TGGGTTCAGAAGGAGTCCAAGGCCCTCCAGGGCAACAGGGCAAGAAAGGGACCCTGGGATTTCCTGGGCT TAATGGATTCCAAGGAATTGAGGGTCAAAAGGGTGACATTGGCCTGCCAGGCCCAGATGTTTTCATCGAT ATAGATGGTGCTGTGATCTCAGGTAATCCTGGAGATCCTGGTGTACCTGGCCTCCCAGGCCTTAAAGGAG ATGAAGGCATCCAAGGCCTACGTGGCCCTTCTGGTGTCCCTGGATTGCCAGCATTATCAGGTGTCCCAGG AGCCCTAGGGCCTCAGGGATTTCCAGGGCTGAAGGGGGACCAAGGAAACCCAGGCCGTACCACAATTGGA GCAGCTGGCCTCCCTGGCAGAGATGGTTTGCCAGGCCCACCAGGTCCACCAGGCCCACCTAGTCCAGAAT TTGAGACTGAAACTCTACACAACAAAGAGTCAGGGTTCCCTGGTCTCCGAGGAGAACAAGGTCCAAAAGG AAACCTAGGCCTCAAAGGAATAAAAGGAGACTCAGGTTTCTGTGCTTGTGACGGTGGTGTTCCCAACACT GGACCACCCGGGGAACCAGGCCCACCTGGTCCATGGGGTCTCATAGGCCTTCCAGGCCTTAAAGGAGCCA GAGGAGATCGAGGCTCTGGGGGTGCACAGGGCCCAGCAGGGGCTCCAGGCTTAGTTGGGCCTCTGGGTCC TTCAGGACCCAAAGGAAAGAAGGGGGAACCAATTCTCAGTACAATCCAAGGAATGCCAGGAGATCGGGGT GATTCTGGCTCCCAGGGCTTCCGTGGTGTAATAGGAGAACCAGGCAAGGACGGAGTACCAGGTTTACCAG GTCTGCCAGGCCTTCCGGGTGATGGTGGACAGGGCTTCCCAGGTGAAAAGGGGTTACCTGGACTTCCTGG TGAAAAAGGCCATCCTGGTCCACCTGGCCTCCCAGGAAATGGGTTACCAGGACTTCCTGGACCCCGTGGG CTTCCTGGAGATAAAGGCAAGGATGGATTACCGGGACAACAAGGCCTTCCCGGATCTAAGGGAATCACCC TGCCCTGTATTATTCCTGGGTCATACGGTCCATCAGGATTTCCAGGCACTCCCGGATTCCCAGGCCCTAA AGGGTCTCGAGGCCTCCCTGGGACCCCAGGCCAGCCTGGGTCAAGTGGAAGTAAAGGAGAGCCAGGGAGT CCAGGATTGGTTCATCTTCCTGAATTACCAGGATTTCCTGGACCTCGTGGGGAGAAGGGCTTGCCTGGGT TTCCTGGGCTCCCTGGAAAAGATGGCTTGCCTGGGATGATTGGCAGTCCAGGCTTACCTGGTTCCAAGGG AGCCACTGGTGACATCTTTGGTGCTGAAAATGGTGCTCCGGGGGAACAAGGCCTACAAGGATTAACAGGG CACAAAGGATTTCTTGGAGACTCTGGCCTTCCAGGACTCAAGGGTGTGCACGGGAAGCCTGGCTTACTAG GCCCCAAAGGTGAGCGGGGCAGCCCTGGGACACCAGGACAGGTGGGACAGCCAGGCACCCCAGGATCTAG TGGTCCATATGGCATCAAGGGCAAATCTGGGCTCCCAGGAGCACCAGGCTTCCCAGGCATCTCAGGACAT CCTGGAAAGAAAGGAACAAGAGGCAAGAAAGGTCCTCCTGGATCAATTGTAAAGAAAGGGCTGCCAGGGC TAAAAGGCCTTCCTGGAAATCCAGGCCTAGTAGGACTGAAAGGAAGCCCAGGCTCTCCAGGGGTCGCTGG GTTGCCAGCCCTCTCTGGACCCAAGGGAGAGAAGGGGTCTGTTGGATTCGTAGGTTTTCCAGGAATACCA GGTCTGCCTGGTATTCCTGGAACAAGAGGATTAAAAGGAATTCCAGGATCAACTGGAAAAATGGGACCAT CTGGACGTGCTGGTACTCCTGGTGAAAAGGGAGACAGAGGCAATCCGGGGCCAGTCGGAATACCTAGTCC AAGACGTCCAATGTCAAACCTTTGGCTCAAAGGAGACAAAGGCTCTCAAGGCTCAGCCGGATCCAATGGA TTTCCTGGGCCAAGAGGTGACAAAGGAGAGGCTGGTCGACCTGGACCACCAGGCCTACCTGGAGCTCCTG GCCTCCCAGGCATTATCAAAGGAGTTAGTGGAAAGCCAGGGCCCCCTGGCTTCATGGGAATCCGGGGCTT ACCTGGCCTGAAGGGGTCCTCTGGGATCACAGGTTTCCCAGGAATGCCAGGAGAAAGTGGTTCACAAGGT ATCAGAGGGTCGCCTGGACTCCCAGGAGCATCTGGTCTCCCAGGCCTGAAAGGAGACAACGGCCAGACAG TTGAAATTTCCGGTAGCCCAGGACCCAAGGGACAGCCTGGCGAATCTGGTTTTAAAGGCACAAAAGGAAG AGATGGACTAATAGGCAATATAGGCTTCCCTGGAAACAAAGGTGAAGATGGAAAAGTTGGTGTTTCTGGA GATGTTGGCCTTCCTGGAGCTCCAGGATTTCCAGGAGTTGCCGGCATGAGAGGAGAACCAGGACTTCCAG GTTCTTCTGGTCACCAAGGGGCAATTGGGCCTCTAGGATCCCCCGGATTAATAGGACCCAAAGGACCTAG TATCACCGGTGTGCCTGGGCCTGCTGGTCTCCCTGGACCCAAAGGAGAAAAAGGATATCCAGGAATTGGC ATCGGAGCTCCAGGGAAGCCGGGCCTGAGAGGGCAAAAAGGTGATCGAGGTTTCCCAGGTCTCCAGGGCC CTGCTGGTCTCCCCGGTGCCCCAGGCATCTCCTTGCCCTCACTCATAGCAGGACAGCCTGGTGACCCCGG GCGACCAGGCCTAGATGGAGAACGAGGCCGCCCAGGCCCCGCTGGACCCCCAGGTCCCCCTGGGCCATCC TCGAATCAAGGCGACACCGGAGACCCTGGCTTCCCTGGAATTCCTGGACCTAAAGGGCCTAAGGGAGACC AAGGAATTCCAGGTTTTTCTGGCCTCCCTGGAGAGCTAGGACTGAAAGGCATGAGAGGTGAGCCTGGCTT CATGGGGACTCCAGGCAAGGTTGGGCCACCTGGAGACCCAGGATTTCCCGGAATGAAGGGGAAGGCAGGG CCAAGAGGCTCTTCTGGCCTCCAAGGTGATCCTGGACAAACACCAACTGCAGAAGCTGTCCAGGTTCCTC CTGGACCCTTGGGTCTACCAGGGATCGATGGCATCCCTGGCCTCACTGGGGACCCTGGGGCTCAAGGCCC TGTAGGCCTACAAGGCTCCAAAGGTTTACCTGGCATCCCCGGTAAAGATGGCCCCAGTGGGCTCCCAGGC CCACCTGGGGCTCTTGGTGATCCTGGTCTGCCTGGACTGCAAGGCCCTCCAGGATTTGAAGGAGCTCCAG GGCAGCAAGGCCCCTTCGGGATGCCTGGAATGCCTGGCCAGAGCATGAGAGTGGGCTACACGTTGGTAAA GCACAGCCAGTCGGAACAGGTGCCCCCGTGTCCCATCGGGATGAGCCAGCTGTGGGTGGGGTACAGCTTA CTGTTTGTGGAGGGGCAAGAGAAAGCCCACAACCAGGACCTGGGCTTTGCTGGCTCCTGTCTGCCCCGCT TCAGCACCATGCCCTTCATCTACTGCAACATCAACGAGGTGTGCCACTATGCCAGGCGCAATGATAAATC TTACTGGCTCTCCACTACCGCCCCTATCCCCATGATGCCCGTCAGCCAGACCCAGATTCCCCAGTACATC AGCCGCTGCTCTGTGTGTGAGGCACCCTCGCAAGCCATTGCTGTGCACAGCCAGGACATCACCATCCCGC AGTGCCCCCTGGGCTGGCGCAGCCTCTGGATTGGGTACTCTTTCCTCATGCACACTGCCGCTGGTGCCGA GGGTGGAGGCCAGTCCCTGGTCTCACCTGGCTCCTGCCTAGAGGACTTTCGGGCCACTCCTTTCATCGAA TGCAGTGGTGCCCGAGGCACCTGCCACTACTTTGCAAACAAGTACAGTTTCTGGTTGACCACAGTGGAGG AGAGGCAGCAGTTTGGGGAGTTGCCTGTGTCTGAAACGCTGAAAGCTGGGCAGCTCCACACTCGAGTCAG TCGCTGCCAGGTGTGTATGAAAAGCCTGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001287759
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287759.1, NP_001274688.1
RefSeq Size	6651 bp
RefSeq ORF	5001 bp
Locus ID	1288
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
Gene Summary	This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (4) lacks several alternate in-frame exons compared to variant 3. The resulting protein (isoform 4) is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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