Collagen V (COL5A1) (NM_001278074) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EDSC; EDSCL1; FMDMF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278074, the custom clone sequence may differ by one or more nucleotides ATGGACGTCCATACCCGCTGGAAAGCGCGCAGCGCGCTCCGCCCGGGCGCCCCGCTGCTGCCCCCGCTGC TGCTGCTGCTGCTGTGGGCGCCGCCTCCGAGCCGCGCAGCTCAGCCAGCAGATCTCCTGAAGGTTCTAGA TTTTCACAACTTGCCTGATGGAATAACAAAGACAACAGGCTTTTGCGCCACGCGGCGATCTTCCAAAGGC CCGGATGTCGCTTACAGAGTCACCAAAGACGCGCAGCTCAGCGCACCCACCAAGCAGCTGTACCCTGCGT CTGCATTTCCCGAGGACTTCTCCATCCTAACAACTGTGAAAGCCAAGAAAGGCAGCCAGGCCTTCCTGGT CTCCATCTACAACGAGCAGGGTATCCAGCAGATTGGGCTGGAGCTGGGCCGCTCTCCCGTCTTCCTCTAC GAGGACCACACGGGGAAGCCTGGCCCGGAAGACTACCCCCTCTTCCGGGGCATCAACCTGTCAGATGGCA AGTGGCACAGAATTGCTCTCAGCGTCCACAAGAAAAATGTCACCTTGATCCTCGACTGTAAAAAGAAGAC CACCAAATTCCTCGACCGCAGCGACCACCCCATGATCGACATCAATGGCATCATCGTGTTTGGCACCCGG ATCCTGGATGAGGAGGTGTTTGAGGGTGACATCCAGCAGCTGCTCTTTGTCTCGGACCACCGGGCAGCTT ATGATTACTGTGAGCACTACAGCCCTGACTGTGACACCGCAGTACCTGACACCCCACAGTCGCAGGACCC CAATCCAGATGAATATTACACGGAAGGAGACGGCGAGGGTGAGACCTATTACTACGAATACCCCTACTAC GAAGACCCCGAAGACCTAGGGAAGGAGCCCACCCCCAGCAAGAAGCCCGTGGAAGCTGCCAAAGAAACCA CAGAGGTCCCCGAGGAGCTGACCCCGACCCCCACGGAAGCTGCTCCCATGCCTGAAACCAGTGAAGGGGC TGGGAAGGAAGAGGACGTCGGCATCGGGGACTATGACTACGTGCCCAGTGAGGACTACTACACGCCCTCA CCGTATGATGACCTCACCTATGGCGAGGGGGAGGAGAACCCCGACCAGCCCACAGACCCAGGCGCTGGGG CCGAAATTCCCACCAGCACCGCCGACACCTCCAACTCCTCCAATCCAGCTCCGCCTCCAGGGGAAGGTGC GGATGACTTGGAGGGGGAGTTCACTGAGGAAACGATCCGGAACCTTGACGAGAACTACTACGACCCCTAC TACGACCCCACCAGCTCCCCGTCGGAGATCGGGCCGGGAATGCCGGCGAACCAGGATACCATCTATGAAG GGATTGGAGGACCTCGGGGCGAGAAAGGCCAAAAGGGAGAACCAGCGATTATCGAGCCGGGCATGCTCAT CGAGGGCCCGCCTGGCCCAGAAGGCCCCGCGGGTCTTCCCGGACCTCCAGGAACCATGGGTCCCACTGGC CAAGTCGGGGACCCTGGAGAAAGGGGCCCCCCTGGACGCCCAGGCCTTCCTGGGGCCGATGGCCTGCCCG GTCCTCCAGGAACCATGCTCATGCTGCCCTTCCGGTTTGGAGGTGGCGGCGATGCGGGCTCCAAAGGCCC CATGGTCTCAGCCCAGGAGTCCCAGGCGCAAGCCATTCTCCAGCAGGCCAGGTTGGCACTGAGGGGACCA GCTGGCCCGATGGGTCTCACAGGGAGACCTGGCCCTGTGGGTCCCCCTGGGAGCGGAGGTTTGAAGGGCG AGCCGGGAGACGTGGGGCCTCAGGGTCCTCGAGGTGTGCAAGGCCCGCCTGGTCCGGCCGGGAAGCCCGG AAGACGGGGTCGGGCTGGGAGTGATGGAGCCAGAGGAATGCCTGGACAAACTGGCCCCAAGGGTGACCGG GGTTTCGACGGCCTGGCTGGGTTGCCAGGCGAGAAGGGCCACAGGGGTGACCCTGGTCCTTCCGGCCCAC CAGGACCTCCGGGAGACGATGGAGAAAGGGGTGACGACGGAGAAGTTGGGCCCAGGGGGCTGCCTGGGGA GCCCGGGCCACGTGGTCTGCTTGGGCCGAAGGGGCCCCCAGGTCCTCCCGGACCTCCCGGTGTCACGGGT ATGGACGGCCAGCCGGGGCCAAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGCCCCCCAGGACAGCAGG GTAATCCAGGCGCCCAGGGTCTTCCAGGCCCCCAGGGTGCAATTGGTCCTCCAGGAGAAAAGGGTCCCTT GGGGAAACCAGGCCTTCCAGGAATGCCCGGTGCTGACGGACCCCCGGGACACCCTGGCAAAGAAGGCCCT CCAGGAGAGAAAGGAGGTCAGGGTCCACCTGGCCCCCAGGGTCCGATTGGCTACCCAGGTCCTCGAGGAG TCAAGGGGGCCGATGGCATCCGTGGTCTGAAGGGCACAAAGGGCGAGAAGGGTGAAGACGGCTTTCCTGG GTTTAAAGGAGACATGGGCATCAAGGGTGATCGGGGGGAGATCGGCCCACCCGGTCCCAGGGGAGAAGAT GGCCCTGAAGGCCCAAAGGGTCGCGGAGGTCCCAATGGTGACCCCGGTCCTCTGGGACCCCCTGGGGAGA AGGGAAAACTCGGAGTCCCAGGGTTACCAGGGTATCCAGGAAGACAAGGACCAAAGGGCTCTATTGGATT CCCTGGATTTCCTGGCGCCAATGGAGAGAAGGGCGGCAGGGGGACCCCTGGAAAGCCAGGACCGCGGGGG CAGCGAGGCCCAACGGGTCCGAGGGGTGAAAGAGGCCCCCGGGGCATCACTGGGAAGCCTGGCCCCAAGG GCAACTCCGGAGGTGACGGCCCAGCTGGCCCTCCTGGTGAACGGGGACCCAATGGACCCCAAGGACCCAC AGGATTTCCTGGACCAAAGGGCCCCCCTGGCCCTCCAGGCAAGGATGGACTCCCAGGACACCCTGGACAG AGAGGCGAGACTGGTTTCCAAGGCAAGACCGGCCCTCCAGGCCCCCCCGGCGTGGTCGGCCCTCAGGGTC CCACGGGAGAAACGGGCCCAATGGGTGAGCGTGGCCACCCTGGGCCCCCTGGACCCCCCGGTGAACAGGG GCTTCCGGGCCTTGCTGGAAAAGAAGGGACGAAGGGTGACCCAGGCCCTGCAGGCCTCCCTGGGAAAGAT GGCCCTCCAGGATTACGTGGTTTCCCTGGGGACCGAGGGCTTCCTGGTCCAGTGGGAGCTCTTGGACTGA AAGGCAATGAAGGGCCCCCTGGCCCACCAGGCCCTGCGGGATCTCCAGGGGAGAGAGGTCCAGCTGGAGC CGCTGGGCCCATCGGAATTCCAGGGAGACCTGGGCCCCAGGGACCCCCAGGGCCGGCAGGAGAGAAAGGG GCTCCTGGCGAGAAAGGCCCACAAGGCCCAGCTGGCCGAGACGGTCTCCAGGGGCCTGTGGGGCTCCCGG GTCCAGCTGGCCCTGTGGGTCCCCCTGGAGAAGACGGAGATAAGGGAGAGATCGGGGAGCCGGGGCAGAA AGGAAGCAAGGGGGACAAAGGAGAACAGGGTCCTCCTGGGCCTACAGGTCCTCAAGGCCCCATCGGACAG CCAGGCCCCTCTGGAGCTGACGGCGAGCCGGGGCCTCGGGGCCAGCAGGGCCTTTTCGGGCAGAAAGGTG ATGAAGGTCCCAGAGGCTTTCCTGGACCCCCTGGGCCAGTGGGGCTGCAGGGTTTGCCAGGACCTCCAGG CGAGAAGGGTGAGACAGGAGACGTGGGCCAGATGGGCCCCCCGGGTCCCCCTGGCCCCCGAGGACCCTCC GGAGCTCCAGGTGCTGATGGCCCACAAGGTCCCCCAGGTGGAATAGGAAACCCTGGTGCAGTGGGAGAGA AGGGCGAGCCTGGCGAAGCAGGTGAGCCTGGCCTTCCGGGAGAAGGCGGCCCCCCGGGACCCAAAGGAGA AAGGGGAGAGAAGGGCGAGTCAGGCCCTTCAGGTGCTGCCGGACCCCCTGGACCCAAAGGCCCTCCCGGA GATGATGGTCCCAAAGGCAGCCCTGGCCCAGTGGGTTTTCCTGGAGATCCTGGCCCCCCCGGAGAGCCTG GCCCCGCGGGTCAAGATGGTCCCCCTGGTGACAAAGGAGATGATGGTGAACCCGGGCAGACGGGATCCCC CGGCCCTACTGGTGAACCAGGTCCATCGGGGCCTCCAGGAAAAAGGGGTCCCCCAGGCCCCGCAGGCCCC GAAGGCAGACAGGGAGAGAAAGGGGCCAAGGGAGAAGCCGGCTTGGAAGGCCCTCCTGGGAAGACTGGCC CCATCGGCCCCCAGGGGGCCCCTGGGAAGCCCGGACCGGATGGCCTTCGAGGGATCCCTGGCCCTGTGGG AGAACAAGGTCTCCCAGGATCCCCAGGCCCGGACGGTCCCCCCGGCCCCATGGGTCCCCCAGGACTTCCC GGCCTCAAAGGAGATTCTGGTCCCAAAGGTGAAAAGGGTCATCCAGGCCTGATCGGGCTCATCGGTCCTC CGGGTGAACAGGGTGAGAAGGGCGACCGTGGTCTCCCTGGCCCCCAGGGCTCCTCCGGTCCTAAGGGAGA ACAGGGTATCACTGGTCCTTCTGGCCCGATTGGGCCTCCTGGGCCCCCTGGCCTGCCGGGTCCGCCTGGT CCAAAAGGTGCTAAGGGCTCCTCGGGTCCAACTGGCCCGAAGGGTGAGGCAGGCCACCCAGGACCCCCAG GCCCCCCGGGCCCCCCGGGAGAGGTCATCCAGCCCCTGCCAATCCAGGCATCCAGGACGCGGCGGAACAT CGACGCCAGCCAGCTGCTGGACGACGGGAATGGCGAGAACTACGTGGACTACGCGGACGGCATGGAAGAG ATCTTCGGCTCTCTCAACTCTCTGAAGCTGGAGATTGAGCAGATGAAACGGCCCCTGGGCACGCAGCAGA ACCCCGCCCGCACCTGCAAGGACCTGCAGCTCTGCCACCCCGACTTCCCAGATGGTGAATACTGGGTCGA TCCTAACCAAGGATGCTCCAGGGATTCCTTCAAGGTTTACTGCAACTTCACAGCCGGGGGGTCGACATGC GTCTTCCCTGACAAGAAGTCCGAAGGGAGTAAAATGGCCCGCTGGCCCAAAGAGCAGCCTTCCACCTGGT ATAGTCAGTACAAGCGGGGGTCCCTGCTCTCCTATGTGGACGCCGAGGGCAACCCTGTGGGTGTGGTACA GATGACCTTCCTGCGGCTGCTGAGCGCCTCTGCCCACCAGAACGTCACCTACCACTGCTACCAGTCAGTG GCCTGGCAGGACGCAGCCACGGGCAGCTACGACAAGGCCCTCCGCTTCCTGGGCTCCAACGACGAGGAGA TGTCCTATGACAACAACCCCTACATCCGCGCCCTGGTGGACGGCTGTGCTACCAAGAAAGGCTACCAGAA GACGGTTCTGGAGATCGACACCCCCAAAGTGGAGCAGGTGCCCATCGTGGACATCATGTTCAATGACTTC GGTGAAGCGTCACAGAAATTTGGATTTGAAGTGGGGCCGGCTTGCTTCATGGGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001278074
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278074.1, NP_001265003.1
RefSeq Size	8455 bp
RefSeq ORF	5517 bp
Locus ID	1289
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion
Gene Summary	This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] Transcript Variant: This variant (2) contains an alternate in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 2 which is of the same length but contains an alternate segment, compared to isoform 1. This variant is based on experimental data in PMID 22149965 and is supported by partial transcripts and RNA-seq data.

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