MAST4 (NM_001290226) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290226, the custom clone sequence may differ by one or more nucleotides ATGAAAGCCCAGCGGGAAAGGCTACAGATTCCGGGGCTGACCTTGGATTGCCGAACAAGCAACCGGAAAA GCTTAATAGGCAATGGGCAGTCACCAGCATTGCCTCGACCACACTCACCTCTCTCTGCTCATGCAGGAAA TAGCCCTCAAGATAGTCCAAGAAATTTCTCCCCCAGTGCCTCAGCCCATTTTTCATTTGCACGGAGAAAT GACAGGACTGATGGACGCCGCTGGTCGTTGGCTTCTCTCCCTTCCTCTGGCTATGGGACAAACACACCCA GCTCTACGGTCTCTTCATCCTGTTCCTCCCAGGAGAAGTTGCATCAGTTACCATACCAACCAACACCAGA CGAGTTACACTTCTTATCAAAACATTTCTGTACCACCGAAAGCATCGCCACTGAGAACAGATGCAGGAAC ACGCCGATGCGCCCCCGTTCCCGAAGTCTGAGCCCTGGACGTTCTCCCGCCTGCTGTGACCATGAAATAA TTATGATGAACCATGTCTACAAAGAAAGGTTCCCAAAGGCTACAGCTCAGATGGAAGAACGTCTAAAGGA AATTATCACCAGCTACTCTCCTGACAACGTTCTACCCTTAGCAGATGGAGTGCTTAGTTTCACTCACCAC CAGATTATTGAACTGGCTCGAGATTGCTTGGATAAATCCCACCAGGGCCTCATCACCTCACGATACTTCC TTGAATTACAGCACAAATTAGATAAGTTGCTACAGGAGGCTCATGATCGTTCAGAAAGTGGAGAATTGGC ATTTATTAAACAACTAGTTCGAAAGATCCTAATTGTTATTGCCCGCCCTGCTCGGTTATTAGAGTGCCTG GAATTTGATCCGGAAGAATTTTACTACCTATTGGAAGCAGCAGAAGGCCATGCCAAAGAAGGACAGGGTA TTAAAACCGACATTCCCAGGTACATCATTAGCCAACTGGGACTCAATAAGGATCCCTTGGAAGAAATGGC TCATTTGGGAAACTACGATAGTGGGACAGCAGAAACACCAGAAACAGATGAATCAGTGAGTAGCTCTAAT GCCTCCCTGAAACTTCGAAGGAAACCTCGGGAAAGTGATTTTGAAACGATTAAATTGATTAGCAATGGAG CCTATGGGGCAGTCTACTTTGTTCGGCATAAAGAATCCCGGCAGAGGTTTGCCATGAAGAAGATTAATAA ACAGAACCTCATCCTTCGAAACCAGATCCAGCAGGCCTTTGTGGAGCGGGATATCCTGACTTTTGCAGAA AACCCCTTTGTTGTCAGCATGTATTGCTCCTTTGAAACAAGGCGCCACTTGTGCATGGTCATGGAATATG TGGAAGGGGGAGACTGTGCTACTTTAATGAAAAACATGGGTCCTCTCCCTGTTGATATGGCCAGAATGTA CTTTGCTGAGACGGTCTTGGCCTTGGAATATTTACATAATTATGGAATTGTACACAGGGATTTGAAACCA GACAACTTGTTGGTTACCTCCATGGGGCACATAAAGCTGACAGATTTTGGATTATCTAAGGTGGGACTAA TGAGCATGACTACCAACCTTTACGAGGGTCATATTGAGAAGGATGCTAGAGAGTTCCTGGATAAACAGGT CTGTGGCACACCTGAATACATTGCACCAGAAGTGATTCTGAGGCAGGGTTATGGAAAGCCGGTGGACTGG TGGGCCATGGGGATTATCCTCTATGAATTTCTGGTTGGATGCGTGCCATTCTTTGGGGATACTCCAGAGG AGCTATTTGGACAAGTCATCAGTGATGAGATCAACTGGCCTGAGAAGGATGAGGCACCCCCACCTGATGC CCAGGATCTGATTACCTTACTCCTCAGGCAGAATCCCCTGGAGAGGCTGGGAACAGGTGGTGCATATGAA GTCAAACAGCATCGATTCTTCCGTTCTTTAGACTGGAACAGTTTGCTGAGACAGAAGGCAGAATTTATTC CCCAACTGGAATCTGAGGATGACACAAGTTATTTTGATACTCGGTCTGAGAAGTATCATCATATGGAAAC GGAGGAAGAAGATGACACAAATGATGAAGACTTTAATGTGGAAATAAGGCAGTTTTCTTCATGTTCACAC AGGTTTTCAAAAGTTTTCAGCAGTATAGATCGAATCACTCAGAATTCAGCAGAAGAGAAGGAAGACTCTG TGGACAAAACCAAAAGCACCACCTTGCCATCCACAGAAACACTGAGCTGGAGTTCAGAATATTCTGAAAT GCAACAGCTATCAACATCCAACTCTTCAGATACTGAAAGCAACAGACATAAACTCAGTTCTGGCCTACTT CCCAAACTGGCTATTTCAACAGAGGGAGAGCAAGATGAAGCTGCCTCCTGCCCTGGAGACCCCCATGAGG AGCCAGGAAAGCCAGCCCTTCCTCCTGAAGAGTGTGCCCAGGAGGAGCCTGAGGTCACCACCCCAGCCAG CACCATCAGCAGCTCCACCCTGTCAGTTGGCAGTTTTTCAGAGCACTTGGATCAGATAAATGGACGAAGC GAGTGTGTGGACAGTACAGATAATTCCTCAAAGCCATCCAGTGAACCCGCTTCTCACATGGCTCGGCAGC GATTAGAAAGCACAGAAAAAAAGAAAATCTCGGGGAAAGTCACAAAGTCCCTCTCTGCCAGTGCTCTTTC CCTCATGATCCCAGGAGATATGTTTGCTGTTTCCCCTCTGGGAAGTCCAATGTCTCCCCATTCCCTGTCC TCGGACCCTTCTTCTTCACGAGATTCCTCTCCCAGCCGAGATTCCTCAGCAGCTTCTGCCAGTCCACATC AGCCGATTGTGATCCACAGTTCGGGGAAGAACTACGGCTTTACCATCCGAGCCATCCGGGTGTATGTGGG AGACAGTGACATCTATACAGTGCACCATATCGTCTGGAATGTAGAAGAAGGAAGTCCGGCATGCCAGGCA GGACTGAAGGCTGGAGATCTTATCACTCACATCAATGGAGAACCAGTGCATGGACTTGTCCACACAGAAG TTATAGAACTCCTACTGAAGAGTGGGAATAAGGTGTCAATCACTACTACCCCATTTGAAAACACATCAAT CAAAACTGGACCAGCCAGGAGAAACAGCTATAAGAGCCGGATGGTGAGGCGGAGCAAGAAATCCAAGAAG AAAGAAAGTCTCGAAAGGAGGAGATCTCTTTTCAAAAAGCTAGCCAAGCAGCCTTCTCCTTTACTCCACA CCAGCCGAAGTTTCTCCTGCTTGAACAGATCCCTGTCATCGGGTGAGAGCCTCCCAGGTTCCCCCACTCA TAGCTTGTCTCCCCGGTCTCCAACACCAAGCTACCGCTCCACCCCTGACTTCCCATCTGGTACTAATTCC TCCCAGAGCAGCTCCCCTAGTTCTAGTGCCCCCAATTCCCCAGCAGGGTCCGGGCACATCCGGCCCAGCA CTCTCCACGGTCTTGCACCCAAACTCGGCGGGCAGCGGTACCGGTCCGGAAGGCGAAAGTCCGCCGGCAA CATCCCACTGTCCCCGCTGGCCCGGACGCCCTCTCCAACCCCGCAACCCACCTCCCCGCAGCGGTCACCA TCCCCTCTTCTGGGACACTCACTGGGCAATTCCAAGATCGCGCAAGCCTTTCCCAGCAAGATGCACTCCC CGCCCACCATCGTCAGACACATCGTGAGGCCCAAGAGTGCGGAGCCCCCCAGGTCCCCGCTGCTCAAGCG CGTGCAGTCCGAGGAGAAGCTGTCGCCCTCTTACGGCAGTGACAAGAAGCACCTGTGCTCCCGCAAGCAC AGCCTGGAGGTGACCCAAGAGGAGGTGCAGCGGGAGCAGTCCCAGCGGGAGGCGCCGCTGCAGAGCCTGG ATGAGAACGTGTGCGACGTGCCGCCGCTCAGCCGCGCCCGGCCAGTGGAGCAAGGCTGCCTGAAACGCCC AGTCTCCCGGAAGGTGGGCCGCCAGGAGTCTGTGGACGACCTGGACCGCGACAAGCTGAAGGCCAAGGTG GTGGTGAAGAAAGCAGACGGCTTCCCAGAGAAACAGGAATCCCACCAGAAATCCCATGGACCCGGGAGTG ATTTGGAAAACTTTGCTCTGTTTAAGCTGGAAGAGAGAGAGAAGAAAGTCTATCCGAAGGCTGTGGAAAG GTCAAGTACTTTTGAAAACAAAGCGTCTATGCAGGAGGCGCCACCGCTGGGCAGCCTGCTGAAGGATGCT CTTCACAAGCAGGCCAGCGTGCGCGCCAGCGAGGGTGCGATGTCGGATGGCCGGGTGCCTGCGGAGCACC GCCAGGGTGGCGGGGACTTCAGACGGGCCCCCGCTCCTGGCACCCTCCAGGATGGTCTCTGCCACTCCCT CGACAGGGGCATCTCTGGGAAGGGGGAAGGCACGGAGAAGTCCTCCCAGGCCAAGGAGCTTCTCCGATGT GAAAAGTTAGACAGCAAGCTGGCCAACATCGATTACCTCCGAAAGAAAATGTCACTTGAGGACAAAGAGG ACAACCTCTGCCCTGTGCTGAAGCCCAAGATGACAGCTGGCTCCCACGAATGCCTGCCAGGGAACCCAGT CCGACCCACGGGTGGGCAGCAGGAGCCCCCGCCGGCTTCTGAGAGCCGAGCTTTTGTCAGCAGCACCCAT GCAGCTCAGATGAGTGCCGTCTCTTTTGTTCCCCTCAAGGCCTTAACAGGCCGGGTGGACAGTGGAACGG AGAAGCCTGGCTTGGTTGCTCCTGAGTCCCCTGTTAGGAAGAGCCCCTCCGAGTATAAGCTGGAAGGTAG GTCTGTCTCATGCCTGAAGCCGATCGAGGGCACTCTGGACATTGCTCTCCTGTCCGGACCTCAGGCCTCC AAGACAGAACTGCCTTCCCCAGAGTCTGCACAGAGCCCCAGCCCAAGTGGTGACGTGAGGGCCTCTGTGC CACCAGTTCTCCCCAGCAGCAGTGGGAAAAAGAACGATACCACCAGTGCAAGAGAGCTTTCTCCTTCCAG CTTAAAGATGAATAAATCCTACCTGCTGGAGCCTTGGTTCCTGCCCCCCAGCCGAGGTCTCCAGAATTCA CCAGCAGTTTCCCTGCCTGACCCAGAGTTCAAGAGGGACAGGAAAGGTCCCCATCCTACTGCCAGGAGCC CTGGAACAGTCATGGAAAGCAATCCCCAACAGAGAGAGGGCAGCTCCCCTAAACACCAAGACCACACCAC TGACCCCAAGCTTCTGACCTGCCTGGGGCAGAACCTCCACAGCCCTGACCTGGCCAGGCCACGCTGCCCG CTCCCACCTGAAGCTTCCCCCTCAAGGGAGAAGCCAGGCCTGAGGGAATCGTCTGAAAGAGGCCCTCCCA CAGCCAGAAGCGAGCGCTCTGCTGCGAGGGCTGACACATGCAGAGAGCCCTCCATGGAACTGTGCTTTCC AGAAACTGCGAAAACCAGTGACAACTCCAAAAATCTCCTCTCTGTGGGAAGGACCCACCCAGATTTCTAT ACACAGACCCAGGCCATGGAGAAAGCATGGGCGCCGGGTGGGAAAACGAACCACAAAGATGGCCCAGGTG AGGCGAGGCCCCCGCCCAGAGACAACTCCTCTCTGCACTCAGCTGGAATTCCCTGTGAGAAGGAGCTGGG CAAGGTGAGGCGTGGCGTGGAACCCAAGCCCGAAGCGCTTCTTGCCAGGCGGTCTCTGCAGCCACCTGGA ATTGAGAGTGAGAAGAGTGAAAAGCTCTCCAGTTTCCCATCTTTGCAGAAAGATGGTGCCAAGGAACCTG AAAGGAAGGAGCAGCCTCTACAAAGGCATCCCAGCAGCATCCCTCCGCCCCCTCTGACGGCCAAAGACCT GTCCAGCCCGGCTGCCAGGCAGCATTGCAGTTCCCCAAGCCACGCTTCTGGCAGAGAGCCGGGGGCCAAG CCCAGCACTGCAGAGCCCAGCTCGAGCCCCCAGGACCCTCCCAAGCCTGTTGCTGCGCACAGTGAAAGCA GCAGCCACAAGCCCCGGCCTGGCCCTGACCCGGGCCCTCCAAAGACTAAGCACCCCGACCGGTCCCTCTC CTCTCAGAAACCAAGTGTCGGGGCCACAAAGGGCAAAGAGCCTGCCACTCAGTCCCTCGGTGGCTCTAGC AGAGAGGGGAAGGGCCACAGTAAGAGTGGGCCGGATGTGTTTCCTGCTACCCCAGGCTCCCAGAACAAAG CCAGCGATGGGATTGGCCAGGGAGAAGGTGGGCCCTCTGTCCCACTGCACACTGACAGGGCTCCTCTAGA CGCCAAGCCACAACCCACCAGTGGTGGGCGGCCCCTGGAGGTGCTGGAGAAGCCTGTGCATTTGCCAAGG CCGGGACACCCAGGGCCTAGTGAGCCAGCGGACCAGAAACTGTCCGCTGTTGGTGAAAAGCAAACCCTGT CTCCAAAGCACCCCAAACCATCCACTGTGAAAGATTGCCCCACCCTGTGCAAACAGACAGACAACAGACA GACAGACAAAAGCCCGAGTCAGCCGGCCGCCAACACCGACAGAAGGGCGGAAGGGAAGAAATGCACTGAA GCACTTTATGCTCCAGCAGAGGGCGACAAGCTCGAGGCCGGCCTTTCCTTTGTGCATAGCGAGAACCGGT TGAAAGGCGCGGAGCGGCCAGCCGCGGGGGTGGGGAAGGGCTTCCCTGAGGCCAGAGGGAAAGGGCCCGG TCCCCAGAAGCCACCGACGGAGGCAGACAAGCCCAATGGCATGAAACGGTCCCCCTCAGCCACTGGGCAG AGTTCTTTCCGATCCACGGCCCTCCCGGAAAAGTCTCTGAGCTGCTCCTCCAGCTTCCCTGAAACCAGGG CCGGAGTTAGAGAGGCCTCTGCAGCCAGCAGCGACACCTCTTCTGCCAAGGCCGCCGGGGGCATGCTGGA GCTTCCAGCCCCCAGCAACAGGGACCATAGGAAGGCTCAGCCTGCCGGGGAGGGCCGAACCCACATGACA AAGAGTGACTCCCTGCCCTCCTTCCGGGTCTCCACCCTGCCTCTGGAGTCACACCACCCCGACCCAAACA CCATGGGCGGGGCCAGCCACCGGGACAGGGCTCTCTCGGTGACTGCCACCGTAGGGGAAACCAAAGGGAA GGACCCTGCCCCAGCCCAGCCTCCCCCAGCTAGGAAACAGAACGTGGGCAGAGACGTGACCAAGCCATCC CCAGCCCCAAACACTGACCGCCCCATCTCTCTTTCTAATGAGAAGGACTTTGTGGTACGGCAGAGGCGGG GGAAAGAGAGTTTGCGTAGCAGCCCTCACAAAAAGGCCTTGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290226
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290226.1, NP_001277155.1
RefSeq Size	10176 bp
RefSeq ORF	7254 bp
Locus ID	375449
UniProt ID	O15021
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	This gene encodes a member of the microtubule-associated serine/threonine protein kinases. The proteins in this family contain a domain that gives the kinase the ability to determine its own scaffold to control the effects of their kinase activities. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (4) represents use of an alternate promoter, differs in the 5' UTR, uses a downstream start codon, and has an alternate in-frame exon compared to variant 3. The encoded protein (isoform d) has a shorter and distinct N-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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