CENPE (NM_001286734) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CENP-E; KIF10; MCPH13; PPP1R61
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286734, the custom clone sequence may differ by one or more nucleotides ATGGCGGAGGAAGGAGCCGTGGCCGTCTGCGTGCGAGTGCGGCCGCTGAACAGCAGAGAAGAATCACTTG GAGAAACTGCCCAAGTTTACTGGAAAACTGACAATAATGTCATTTATCAAGTTGATGGAAGTAAATCCTT CAATTTTGATCGTGTCTTTCATGGTAATGAAACTACCAAAAATGTGTATGAAGAAATAGCAGCACCAATC ATCGATTCTGCCATACAAGGCTACAATGGTACTATATTTGCCTATGGACAGACTGCTTCAGGAAAAACAT ATACCATGATGGGTTCAGAAGATCATTTGGGAGTTATACCCAGGGCAATTCATGACATTTTCCAAAAAAT TAAGAAGTTTCCTGATAGGGAATTTCTCTTACGTGTATCTTACATGGAAATATACAATGAAACCATTACA GATTTACTCTGTGGCACTCAAAAAATGAAACCTTTAATTATTCGAGAAGATGTCAATAGGAATGTGTATG TTGCTGATCTCACAGAAGAAGTTGTATATACATCAGAAATGGCTTTGAAATGGATTACAAAGGGAGAAAA GAGCAGGCATTATGGAGAAACAAAAATGAATCAAAGAAGCAGTCGTTCTCATACCATCTTTAGGATGATT TTGGAAAGCAGAGAGAAGGGTGAACCTTCTAATTGTGAAGGATCTGTTAAGGTATCCCATTTGAATTTGG TTGATCTTGCAGGCAGTGAAAGAGCTGCTCAAACAGGCGCTGCAGGTGTGCGGCTCAAGGAAGGCTGTAA TATAAATCGAAGCTTATTTATTTTGGGACAAGTGATCAAGAAACTTAGTGATGGACAAGTTGGTGGTTTC ATAAATTATCGAGATAGCAAGTTAACACGAATTCTCCAGAATTCCTTGGGAGGAAATGCAAAGACACGTA TTATCTGCACAATTACTCCAGTATCTTTTGATGAAACACTTACTGCTCTCCAGTTTGCCAGTACTGCTAA ATATATGAAGAATACTCCTTATGTTAATGAGGTATCAACTGATGAAGCTCTCCTGAAAAGGTATAGAAAA GAAATAATGGATCTTAAAAAACAATTAGAGGAGGTTTCTTTAGAGACGCGGGCTCAGGCAATGGAAAAAG ACCAATTGGCCCAACTTTTGGAAGAAAAAGATTTGCTTCAGAAAGTACAGAATGAGAAAATTGAAAACTT AACACGGATGCTGGTGACCTCTTCTTCCCTCACGTTGCAACAGGAATTAAAGGCTAAAAGAAAACGAAGA GTTACTTGGTGCCTTGGCAAAATTAACAAAATGAAGAACTCAAACTATGCAGATCAATTTAATATACCAA CAAATATAACAACAAAAACACATAAGCTTTCTATAAATTTATTACGAGAAATTGATGAATCTGTCTGTTC AGAGTCTGATGTTTTCAGTAACACTCTTGATACATTAAGTGAGATAGAATGGAATCCAGCAACAAAGCTA CTAAATCAGGAGAATATAGAAAGTGAGTTGAACTCACTTCGTGCTGACTATGATAATCTGGTATTAGACT ATGAACAACTACGAACAGAAAAAGAAGAAATGGAATTGAAATTAAAAGAAAAGAATGATTTGGATGAATT TGAGGCTCTAGAAAGAAAAACTAAAAAAGATCAAGAGAATGAACTCAGTTCAAAAGTAGAGCTGCTTAGA GAAAAGGAAGACCAGATTAAGAAGCTACAGGAATACATAGACTCTCAAAAGCTAGAAAATATAAAAATGG ACTTGTCATACTCATTGGAAAGCATTGAAGACCCAAAACAAATGAAGCAGACTCTGTTTGATGCTGAAAC TGTAGCCCTTGATGCCAAGAGAGAATCAGCCTTTCTTAGAAGTGAAAATCTGGAGCTGAAGGAGAAAATG AAAGAACTTGCAACTACATACAAGCAAATGGAAAATGATATTCAGTTATATCAAAGCCAGTTGGAGGCAA AAAAGAAAATGCAAGTTGATCTGGAGAAAGAATTACAATCTGCTTTTAATGAGATAACAAAACTCACCTC CCTTATAGATGGCAAAGTTCCAAAAGATTTGCTCTGTAATTTGGAATTGGAAGGAAAGATTACTGATCTT CAGAAAGAACTAAATAAAGAAGTTGAAGAAAATGAAGCTTTGCGGGAAGAAGTCATTTTGCTTTCAGAAT TGAAATCTTTACCTTCTGAAGTAGAAAGGCTGAGGAAAGAGATACAAGACAAATCTGAAGAGCTCCATAT AATAACATCAGAAAAAGATAAATTGTTTTCTGAAGTAGTTCATAAGGAGAGTAGAGTTCAAGGTTTACTT GAAGAAATTGGGAAAACAAAAGATGACCTAGCAACTACACAGTCGAATTATAAAAGCACTGATCAAGAAT TCCAAAATTTCAAAACCCTTCATATGGACTTTGAGCAAAAGTATAAGATGGTCCTTGAGGAGAATGAGAG AATGAATCAGGAAATAGTTAATCTCTCTAAAGAAGCCCAAAAATTTGATTCGAGTTTGGGTGCTTTGAAG ACCGAGCTTTCTTACAAGACCCAAGAACTTCAGGAGAAAACACGTGAGGTTCAAGAAAGACTAAATGAGA TGGAACAGCTGAAGGAACAATTAGAAAATAGAGATTCTACGCTGCAAACTGTAGAAAGGGAGAAAACACT GATTACTGAGAAACTGCAGCAAACTTTAGAAGAAGTAAAAACTTTAACTCAAGAAAAAGATGATCTAAAA CAACTCCAAGAAAGCTTGCAAATTGAGAGGGACCAACTCAAAAGTGATATTCACGATACTGTTAACATGA ATATAGATACTCAAGAACAATTACGAAATGCTCTTGAGTCTCTGAAACAACATCAAGAAACAATTAATAC ACTAAAATCGAAAATTTCTGAGGAAGTTTCCAGGAATTTGCATATGGAGGAAAATACAGGAGAAACTAAA GATGAATTTCAGCAAAAGATGGTTGGCATAGATAAAAAACAGGATTTGGAAGCTAAAAATACCCAAACAC TAACTGCAGATGTTAAGGATAATGAGATAATTGAGCAACAAAGGAAGATATTTTCTTTAATACAGGAGAA AAATGAACTCCAACAAATGTTAGAGAGTGTTATAGCAGAAAAGGAACAATTGAAGACTGACCTAAAGGAA AATATTGAAATGACCATTGAAAACCAGGAAGAATTAAGACTTCTTGGGGATGAACTTAAAAAGCAACAAG AGATAGTTGCACAAGAAAAGAACCATGCCATAAAGAAAGAAGGAGAGCTTTCTAGGACCTGTGACAGACT GGCAGAAGTTGAAGAAAAACTAAAGGAAAAGAGCCAGCAACTCCAAGAAAAACAGCAACAACTTCTTAAT GTACAAGAAGAGATGAGTGAGATGCAGAAAAAGATTAATGAAATAGAGAATTTAAAGAATGAATTAAAGA ACAAAGAATTGACATTGGAACATATGGAAACAGAGAGGCTTGAGTTGGCTCAGAAACTTAATGAAAATTA TGAGGAAGTGAAATCTATAACCAAAGAAAGAAAAGTTCTAAAGGAATTACAGAAGTCATTTGAAACAGAG AGAGACCACCTTAGAGGATATATAAGAGAAATTGAAGCTACAGGCCTACAAACCAAAGAAGAACTAAAAA TTGCTCATATTCACCTAAAAGAACACCAAGAAACTATTGATGAACTAAGAAGAAGCGTATCTGAGAAGAC AGCTCAAATAATAAATACTCAGGACTTAGAAAAATCCCATACCAAATTACAAGAAGAGATCCCAGTGCTT CATGAGGAACAAGAGTTACTGCCTAATGTGAAAGAAGTCAGTGAGACTCAGGAAACAATGAATGAACTGG AGTTATTAACAGAACAGTCCACAACCAAGGACTCAACAACACTGGCAAGAATAGAAATGGAAAGGCTCAG GTTGAATGAAAAATTTCAAGAAAGTCAGGAAGAGATAAAATCTCTAACCAAGGAAAGAGACAACCTTAAA ACGATAAAAGAAGCCCTTGAAGTTAAACATGACCAGCTGAAAGAACATATTAGAGAAACTTTGGCTAAAA TCCAGGAGTCTCAAAGCAAACAAGAACAGTCCTTAAATATGAAAGAAAAAGACAATGAAACTACCAAAAT CGTGAGTGAGATGGAGCAATTCAAACCCAAAGATTCAGCACTACTAAGGATAGAAATAGAAATGCTCGGA TTGTCCAAAAGACTTCAAGAAAGTCATGATGAAATGAAATCTGTAGCTAAGGAGAAAGATGACCTACAGA GGCTGCAAGAAGTTCTTCAATCTGAAAGTGACCAGCTCAAAGAAAACATAAAAGAAATTGTAGCTAAACA CCTGGAAACTGAAGAGGAACTTAAAGTTGCTCATTGTTGCCTGAAAGAACAAGAGGAAACTATTAATGAG TTAAGAGTGAATCTTTCAGAGAAGGAAACTGAAATATCAACCATTCAAAAGCAGTTAGAAGCAATCAATG ATAAATTACAGAACAAGATCCAAGAGATTTATGAGAAAGAGGAACAATTTAATATAAAACAAATTAGTGA GGTTCAGGAAAAAGTGAATGAACTGAAACAATTCAAGGAGCATCGCAAAGCCAAGGATTCAGCACTACAA AGTATAGAAAGTAAGATGCTCGAGTTGACCAACAGACTTCAAGAAAGTCAAGAAGAAATACAAATTATGA TTAAGGAAAAAGAGGAAATGAAAAGAGTACAGGAGGCCCTTCAGATAGAGAGAGACCAACTGAAAGAAAA CACTAAAGAAATTGTAGCTAAAATGAAAGAATCTCAAGAAAAAGAATATCAGTTTCTTAAGATGACAGCT GTCAATGAGACTCAGGAGAAAATGTGTGAAATAGAACACTTGAAGGAGCAATTTGAGACCCAGAAGTTAA ACCTGGAAAACATAGAAACGGAGAATATAAGGTTGACTCAGATACTACATGAAAACCTTGAAGAAATGAG ATCTGTAACAAAAGAAAGAGATGACCTTAGGAGTGTGGAGGAGACTCTCAAAGTAGAGAGAGACCAGCTC AAGGAAAACCTTAGAGAAACTATAACTAGAGACCTAGAAAAACAAGAGGAGCTAAAAATTGTTCACATGC ATCTGAAGGAGCACCAAGAAACTATTGATAAACTAAGAGGGATTGTTTCAGAGAAAACAAATGAAATATC AAATATGCAAAAGGACTTAGAACACTCAAATGATGCCTTAAAAGCACAGGATCTGAAAATACAAGAGGAA CTAAGAATTGCTCACATGCATCTGAAAGAGCAGCAGGAAACTATTGACAAACTCAGAGGAATTGTTTCTG AGAAGACAGATAAACTATCAAATATGCAAAAAGATTTAGAAAATTCAAATGCTAAATTACAAGAAAAGAT TCAAGAACTTAAGGCAAATGAACATCAACTTATTACGTTAAAAAAAGATGTCAATGAGACACAGAAAAAA GTGTCTGAAATGGAGCAACTAAAGAAACAAATAAAAGACCAAAGCTTAACTCTGAGTAAATTAGAAATAG AGAATTTAAATTTGGCTCAGAAACTTCATGAAAACCTTGAAGAAATGAAATCTGTAATGAAAGAAAGAGA TAATCTAAGAAGAGTAGAGGAGACACTCAAACTGGAGAGAGACCAACTCAAGGAAAGCCTGCAAGAAACC AAAGCTAGAGATCTGGAAATACAACAGGAACTAAAAACTGCTCGTATGCTATCAAAAGAACACAAAGAAA CTGTTGATAAACTTAGAGAAAAAATTTCAGAAAAGACAATTCAAATTTCAGACATTCAAAAGGATTTAGA TAAATCAAAAGATGAATTACAGAAAAAGCAGGACCGACAGAACCACCAAGTAAAACCTGAAAAAAGGTTA CTAAGTGATGGACAACAGCACCTTACGGAAAGCCTGAGAGAAAAGTGCTCTAGAATAAAAGAGCTTTTGA AGAGATACTCAGAGATGGATGATCATTATGAGTGCTTGAATAGATTGTCTCTTGACTTGGAGAAGGAAAT TGAATTCCAAAAAGAGCTTTCAATGAGAGTTAAAGCAAACCTCTCACTTCCCTATTTACAAACCAAACAC ATTGAAAAACTTTTTACTGCAAACCAGAGATGCTCCATGGAATTCCACAGAATCATGAAGAAACTGAAGT ATGTGTTAAGCTATGTTACAAAAATAAAAGAAGAACAACATGAATCCATCAATAAATTTGAAATGGATTT TATTGATGAAGTGGAAAAGCAAAAGGAATTGCTAATTAAAATACAGCACCTTCAACAAGATTGTGATGTA CCATCCAGAGAATTAAGGGATCTCAAATTGAACCAGAATATGGATCTACATATTGAGGAAATTCTCAAAG ATTTCTCAGAAAGTGAGTTCCCTAGCATAAAGACTGAATTTCAACAAGTACTAAGTAATAGGAAAGAAAT GACACAGTTTTTGGAAGAGTGGTTAAATACTCGTTTTGATATAGAAAAGCTTAAAAATGGCATCCAGAAA GAAAATGATAGGATTTGTCAAGTGAATAACTTCTTTAATAACAGAATAATTGCCATAATGAATGAATCAA CAGAGTTTGAGGAAAGAAGTGCTACCATATCCAAAGAGTGGGAACAGGACCTGAAATCACTGAAAGAGAA AAATGAAAAACTATTTAAAAACTACCAAACATTGAAGACTTCCTTGGCATCTGGTGCCCAGGTTAATCCT ACCACACAAGACAATAAGAATCCTCATGTTACATCAAGAGCTACACAGTTAACCACAGAGAAAATTCGAG AGCTGGAAAATTCACTGCATGAAGCTAAAGAAAGTGCTATGCATAAGGAAAGCAAGATTATAAAGATGCA GAAAGAACTTGAGGTGACTAATGACATAATAGCAAAACTTCAAGCCAAAGTTCATGAATCAAATAAATGC CTTGAAAAAACAAAAGAGACAATTCAAGTACTTCAGGACAAAGTTGCTTTAGGAGCTAAGCCATATAAAG AAGAAATTGAAGATCTCAAAATGAAGCTTGTGAAAATAGACCTAGAGAAAATGAAAAATGCCAAAGAATT TGAAAAGGAAATCAGTGCTACAAAAGCCACTGTAGAATATCAAAAGGAAGTTATAAGGCTATTGAGAGAA AATCTCAGAAGAAGTCAACAGGCCCAAGATACCTCAGTGATATCAGAACATACTGATCCTCAGCCTTCAA ATAAACCCTTAACTTGTGGAGGTGGCAGCGGCATTGTACAAAACACAAAAGCTCTTATTTTGAAAAGTGA ACATATAAGGCTAGAAAAAGAAATTTCTAAGTTAAAGCAGCAAAATGAACAGCTAATAAAACAAAAGAAT GAATTGTTAAGCAATAATCAGCATCTTTCCAATGAGGTCAAAACTTGGAAGGAAAGAACCCTTAAAAGAG AGGCTCACAAACAAGTAACTTGTGAGAATTCTCCAAAGTCTCCTAAAGTGACTGGAACAGCTTCTAAAAA GAAACAAATTACACCCTCTCAATGCAAGGAACGGAATTTACAAGATCCTGTGCCAAAGGAATCACCAAAA TCTTGTTTTTTTGATAGCCGATCAAAGTCTTTACCATCACCTCATCCAGTTCGCTATTTTGATAACTCAA GTTTAGGCCTTTGTCCAGAGGTGCAAAATGCAGGAGCAGAGAGTGTGGATTCTCAGCCAGGTCCTTGGCA CGCCTCCTCAGGCAAGGATGTGCCTGAGTGCAAAACTCAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001286734
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286734.1, NP_001273663.1
RefSeq Size	8267 bp
RefSeq ORF	7743 bp
Locus ID	1062
UniProt ID	Q02224
Protein Families	Druggable Genome, Stem cell - Pluripotency
Gene Summary	Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (2) lacks two alternate exons and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. It is unknown whether this isoform (2) is proteolytically processed in the same manner as isoform 1.

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