pIIIa (AC_000017) Virus Tagged ORF Clone

CAT#: VC100009

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 1], codon optimized for human cell expression, AP_000506



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CNY 7,030.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100009 represents NCBI reference of AP_000506 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCAGGATGCTACAGACCCAGCGGTTCGAGCCGCTTTGCAGTCTCAGCCAAGTGGCCTTAATAGCA
CTGATGACTGGAGGCAGGTTATGGATAGGATCATGTCTCTCACCGCAAGAAACCCAGACGCCTTCAGACA
GCAGCCCCAAGCAAATAGACTGAGTGCGATCCTGGAGGCTGTGGTCCCTGCGAGGGCCAATCCTACACAT
GAGAAAGTGTTGGCCATTGTCAATGCCCTGGCGGAGAATCGGGCCATTCGCCCCGATGAGGCCGGCCTGG
TATACGATGCCCTTTTGCAACGGGTGGCTCGGTATAATTCTGGCAACGTGCAGACTAACCTGGACAGACT
GGTGGGGGACGTGAGAGAAGCAGTTGCTCAGCGAGAACGGGCTCAGCAACAGGGCAATCTGGGAAGCATG
GTAGCTCTCAACGCTTTTCTGAGCACCCAGCCTGCAAACGTCCCGCGGGGGCAAGAGGACTACACAAACT
TTGTCTCCGCCCTGAGACTGATGGTCACCGAAACACCTCAGTCAGAGGTCTATCAGTCCGGTCCCGACTA
TTTTTTCCAGACATCCAGGCAGGGCCTCCAGACCGTGAATCTGTCTCAGGCATTTAAGAATCTCCAGGGT
CTGTGGGGTGTTAGGGCGCCGACTGGTGATCGGGCCACGGTCTCCTCTCTGTTGACCCCCAACAGCAGGC
TGCTGCTTCTGTTGATCGCGCCATTCACTGACTCCGGATCCGTTTCCAGGGATACGTATCTCGGCCACCT
GCTTACGCTTTACAGGGAAGCTATTGGCCAAGCGCATGTCGACGAACATACATTCCAGGAGATAACCTCT
GTGTCCAGGGCTCTGGGCCAGGAGGACACAGGGAGTCTGGAAGCCACTTTGAACTATCTGCTTACCAACC
GGCGGCAGAAAATTCCCTCTCTCCATTCCCTCAACTCAGAAGAGGAAAGGATCCTGAGGTATGTTCAGCA
GAGCGTTTCTCTGAACCTGATGAGGGATGGCGTGACCCCAAGTGTGGCCCTTGACATGACAGCCAGGAAC
ATGGAACCTGGAATGTATGCGTCCAACAGACCCTTTATTAATCGCCTGATGGATTATCTGCACCGCGCTG
CCGCAGTGAACCCCGAATACTTCACTAATGCCATCCTGAACCCACACTGGCTTCCGCCACCCGGATTTTA
CACTGGCGGATTCGAAGTCCCAGAAGGCAACGACGGCTTCCTGTGGGATGATATCGACGATAGTGTATTT
TCCCCTCAGCCACAGACACTTCTTGAGCTGCAGCAGAGAGAACAAGCCGAAGCCGCCCTGCGGAAGGAAA
GTTTTCGAAGGCCATCCAGTCTCTCCGATTTGGGCGCGGCTGCACCTCGGTCCGATGCAAGCTCTCCATT
TCCTAGCCTCATCGGATCCCTGACATCCACAAGGACCACCAGGCCAAGACTGTTGGGAGAGGAAGAATAC
CTGAATAACAGCCTGCTTCAGCCACAGCGAGAAAAAAACCTGCCTCCCGCCTTTCCCAACAATGGCATTG
AGTCTCTCGTGGATAAAATGAGTAGATGGAAGACTTACGCACAGGAGCACCGCGATGTCCCTGGCCCCAG
GCCGCCGACTCGGAGGCAGAGACATGACAGGCAACGGGGACTGGTATGGGAGGACGATGATTCAGCCGAT
GACAGCAGCGTCCTGGACCTGGGTGGCTCCGGCAATCCGTTCGCACACCTTCGGCCCCGGTTGGGGCGCA
TGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100009 representing AP_000506
Red=Cloning sites Green=Tags

MMQDATDPAVRAALQSQPSGLNSTDDWRQVMDRIMSLTARNPDAFRQQPQANRLSAILEAVVPARANPTH
EKVLAIVNALAENRAIRPDEAGLVYDALLQRVARYNSGNVQTNLDRLVGDVREAVAQRERAQQQGNLGSM
VALNAFLSTQPANVPRGQEDYTNFVSALRLMVTETPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLQG
LWGVRAPTGDRATVSSLLTPNSRLLLLLIAPFTDSGSVSRDTYLGHLLTLYREAIGQAHVDEHTFQEITS
VSRALGQEDTGSLEATLNYLLTNRRQKIPSLHSLNSEEERILRYVQQSVSLNLMRDGVTPSVALDMTARN
MEPGMYASNRPFINRLMDYLHRAAAVNPEYFTNAILNPHWLPPPGFYTGGFEVPEGNDGFLWDDIDDSVF
SPQPQTLLELQQREQAEAALRKESFRRPSSLSDLGAAAPRSDASSPFPSLIGSLTSTRTTRPRLLGEEEY
LNNSLLQPQREKNLPPAFPNNGIESLVDKMSRWKTYAQEHRDVPGPRPPTRRQRHDRQRGLVWEDDDSAD
DSSVLDLGGSGNPFAHLRPRLGRMF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000017
ORF Size 1755 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000017.1, AP_000506
RefSeq ORF 1755 bp
MW 65.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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